KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Extraction and Identification of Volatile Isothiocyanates from Wasabi using Supercritical Carbon Dioxide

초임계 이산화탄소를 이용한 고추냉이로부터 휘발성 Isothiocyanates류 추출 및 동정

  • Kim, Sung-Jin (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Lee, Min-Kyung (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Back, Sung-Sin (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Chun, Byung-Soo (Faculty of Food Science and Biotechnology, Pukyong National University)
  • 김성진 (부경대학교 식품생명공학부) ;
  • 이민경 (부경대학교 식품생명공학부) ;
  • 백성신 (부경대학교 식품생명공학부) ;
  • 전병수 (부경대학교 식품생명공학부)
  • Published : 2007.06.30
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Abstract

The aim of this study was to identifyisothiocyanates (ITCs) from wasabi (Wasabi japonica Matsum) using supercritical carbon dioxide ($SCO_2$) and to compare the composition in the extracts between $SCO_2$ and organic solvents extraction. A semi-continuous high pressure apparatus was used to extract wasabi (roots, stems and leaves) at following conditions pressure 80$\sim$120 bar, temperature $40\sim50^{\circ}C$. Ether, ethanol, chloroform and dichloromethane were used as organic solvents. The ITCs extracted by means of both separation technologies were analyzed by a gas chromatography system. As the results of study, AITC and ITCs were highly extracted at 40$^{\circ}C$ and 80 bar. To extract AITC from wasabi, $SCO_2$ extraction is more effective than organic solvents extraction, resulted in thermal degeneration and remaining of organic solvents.

본 연구는 고추냉이로부터 항균활성을 가지는 휘발성황 화합물인 AITC를 중점으로 ITCs 류를 $SCO_2$를 이용한 추출 목적으로 하였다. 연구에서 semi-continuous flow 추출장치를 이용해 AITC를 추출한 결과 기존의 에테르나 에탄올 등 유기용매와 비교 시 7.9 AITC (mg)/sample (g)가 추출되어 비슷한 추출율을 보였으며 이는 기존의 유기용매 추출시 문제가 되는 용매회수와 잔존유기용매에 의한 독성 문제 등을 해결할 수 있는 추출법으로써 $SCO_2$추출이 효과적임을 알 수 있다. 또한 AITC의 $SCO_2$ 추출 시 잎과 줄기에서는 40$^{\circ}C$, 80 bar의 조건에서 가장 많은 량이 추출되었으며, 뿌리에서는 40$^{\circ}C$, 120 bar에서 가장 많은 추출량을 나타내었다. 또한 추출시간의 경우 유기용매 추출과 비교했을 때 추출시간을 줄일 수 있어 추출에 소요되는 시간을 절약할 수 있는 좋은 추출방법이다.

Keywords

References

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