Exo-Polysaccharide Production from Liquid Culture of Lentinus edodes

Lentinus edodes 액체배양을 통한 세포외 다당체 생산

  • Lee, Hee-Hwan (Department of Bioengineering and Technology, Kangwon National University) ;
  • Cho, Jae-Youl (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Hong, Eock-Kee (Department of Bioengineering and Technology, Kangwon National University)
  • 이희환 (강원대학교 생물공학과) ;
  • 조재열 (강원대학교 바이오산업공학부) ;
  • 홍억기 (강원대학교 생물공학과)
  • Published : 2007.02.28

Abstract

The optimum liquid culture conditions were investigated for cell growth and polysaccharide production from liquid culture of Lentinus edodes. In flask culture, the optimal medium compositions for the polysaccharide production contained glucose 60 g/L, yeast extract 10 g/L, $KH_2PO_4$ 2.0 g/L, and $MgSO_4{\cdot}7H_2O$ 1.0 g/L. The maximum mycelial growth and polysaccharide production were 11.01 g/L and 1.64 g/L, respectively. In bioreactor, through the variation of aeration in order to increase mycelial growth and polysaccharide production, the maximum mycelial growth and polysaccharide production were 55.9 g/L at 8th day and 7.34 g/L at 7th day of cultivation with 1.5 vvm, respectively.

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