Exo-Polysaccharide Production from Liquid Culture of Lentinus edodes

Lentinus edodes 액체배양을 통한 세포외 다당체 생산

  • Lee, Hee-Hwan (Department of Bioengineering and Technology, Kangwon National University) ;
  • Cho, Jae-Youl (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Hong, Eock-Kee (Department of Bioengineering and Technology, Kangwon National University)
  • 이희환 (강원대학교 생물공학과) ;
  • 조재열 (강원대학교 바이오산업공학부) ;
  • 홍억기 (강원대학교 생물공학과)
  • Published : 2007.02.28


The optimum liquid culture conditions were investigated for cell growth and polysaccharide production from liquid culture of Lentinus edodes. In flask culture, the optimal medium compositions for the polysaccharide production contained glucose 60 g/L, yeast extract 10 g/L, $KH_2PO_4$ 2.0 g/L, and $MgSO_4{\cdot}7H_2O$ 1.0 g/L. The maximum mycelial growth and polysaccharide production were 11.01 g/L and 1.64 g/L, respectively. In bioreactor, through the variation of aeration in order to increase mycelial growth and polysaccharide production, the maximum mycelial growth and polysaccharide production were 55.9 g/L at 8th day and 7.34 g/L at 7th day of cultivation with 1.5 vvm, respectively.


  1. Sung, J. M., Y. B. Yu, and D. Y. Cha (1998), Mushroom Science, p393, Kyo-Hak Publishing Co., Ltd., Seoul
  2. Lee, J. Y. and S. W. Hong (1985), Illustrated Flora & Fauna of Korea, Vol. 28, Ministry of Education, Seoul
  3. Chihara, G. (1985), Immune modulation agents and their mechanisms (Lentinan, a T-cell oriented immunopotentiator), NY and Basel. 19, 409-436
  4. Takehara, M. (1979), Antiviral activity of virus-like particles from Lentinus edodes (Shiitake), Arch Virol. 59, 269-280 https://doi.org/10.1007/BF01317423
  5. Mori, H., K. Aizawa, T. Inakuma, A. Ichii, R. Yamauchi, and K. Kato (1998), Structural analysis of the ${\beta}-D-glucan$ from the fruit-body of Hericium erinaceum, J. Appl. Glycosci. 45, 361-365
  6. Wang Z., D. Luo, and Z. Liang (2004), Structure of polysaccharides from the fruiting body of Hericium erinaccus Pers, Carbohydrose Polymers 57, 241-247 https://doi.org/10.1016/j.carbpol.2004.04.018
  7. Oh-Hashi, T., T. Kataoka, and S. Tsugagoshi (1976), Effect of combinded use of anticancer drugs with a polysaccharide preparation, Krestin, on mouse leukemia P388, Cann. 67, 713
  8. Samamoto, R., T. Niimi, and S. Takahashi (1978), Effect of carbon and nitrogen sources on submerged culture of edible fungi, Agric, BioI. Chem. 52, 75-81
  9. Braun, S. and S. E. Vecht-J.ifshitz (1991), Mycelial morphology and metabolite production, Trends in Biotechnol. 9, 63-68 https://doi.org/10.1016/0167-7799(91)90191-J
  10. Roles, A. J., V. Verg, and R. M. Voncken (1974), Rheology of mycelial broth, Biotechnol. Bioeng. 16, 181-208 https://doi.org/10.1002/bit.260160204
  11. Choi, J. H., S. Y. Kim, D. K. Oh, and J. H. Kim (1998), Optimization of culture conditions for production of a high viscosity polysaccharide, methylan, by Methylobacterium organophilum from methanol, Kor. J. Appl. Microbiol. Biotechnol. 26, 244-249
  12. Yang, S. N., S. W. Beak, and N. K. Kim (2000), Effect of aeration and agitation rates on pullulan production, J. Kor. Indus. Chem. Eng. 38, 556-559
  13. Park, K. S. and B. L. Lee (1997), Extraction and separation of protein-bound polysaccharide by Lentinus edodes, Kor. J. Food Nutr. 10, 503-508
  14. Lee, B. W., G. H. lm, D. W. Kim, K. M. Park, S. H. Son, and T. H. Shon (1993), Culture characteristics and pilot scale fermentation for the submerged mycelial culture of Lentinus edodes, Kor. J. Appl. Microbiol. Biotechnol. 21, 609-614