생명과학회지 (Journal of Life Science)

한국생명과학회 (Korean Society of Life Science)
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배추흰나비 용기 특이 큐티클 단백질의 시공간적 역할

Temporal and Spatial Role of Pupal Stage Specific Cuticle Protein in Artogeia rapae

  • 신명자 (안동대학교 자연과학대학 생명과학과) ;
  • 박정남 (안동대학교 자연과학대학 생명과학과) ;
  • 서을원 (안동대학교 자연과학대학 생명과학과)
  • Shin, Myung-Ja (Dept. of Biological Science, Andong National University) ;
  • Park, Jeong-Nam (Dept. of Biological Science, Andong National University) ;
  • Seo, Eul-Won (Dept. of Biological Science, Andong National University)
  • 발행 : 2007.02.28
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초록

배추흰나비 날개 형성기 동안 용기 특이 큐티클 단백질의 국소부위에 따른 분포와 신생조직합성에 있어 이의 시공간적 역할을 조사하였다. 큐티클에서 27 kd 단백질은 국소 부위에 따른 질적 변화는 거의 없으나 용 초이게 비교적 높은 농도를 보이다가 성충기로 감에 따라 서서히 농도가 낮아지는 양적인 변화를 나타내고 있다. 지방체에서 27 kd 단백질은 용화 후 3일까지는 흉부와 복부에서 전시기에 걸쳐 확인되고 있지만 그 이후 부터는 농도가 감소하는 경향을 나타내고 있다. Western blot 상에서도 27 kd 큐티클 단백질의 흉부와 복부의 국소적인 분포에는 거의 차이가 없으나 용말기와 성충 날개큐티클에서는 동질성을 보여주고 있다. $^{3}H-leucine$은 흉부와 복부 큐티클의 27 kd 단백질에 시공간적인 차이를 두고 통합되고 있으며, 용말기와 우화직전 흉부 큐티클과 복부 큐티클의 27 kd 단백질에서는 확인되지 않았으나 우화직전과 직후의 날개큐티클에 통합되는 패턴을 보여주고 있다. 지방체와 표피에서도 27 kd 단백질에 대한 통합이 두드러지게 나타나고 있다. 27 kd 용기 특이 큐티클 단백질은 국소 부위에 따른 질적, 양적 차이는 나타나지 않았으나 날개 형성기 동안 부분적으로 27 kd 단백질이 포함된 endocuticle이 소화, 재흡수 과정을 통해 성충 날개 큐티클의 형성에 관여할 것으로 생각된다.

Present study aims to investigate the topical distribution of pupal stage specific cuticle protein and its temporal and spatial role during the wing formation of Artogeia rapae. ArCP27(27 kd cuticle protein) was identified as pupal stage specific cuticle protein in cuticle tissues and has not shown any qualitative differences by local portions of body. ArCP27 maintained constant concentration just after pupal ecdysis to 5-day old pupal stage but thereafter decreased. In fat body, ArCP27 was found in both thoracic and abdominal fat body from the last larval to pupal stage. In wing cuticle, ArCP27 began to find from 5-day old pupal stage. Immunologically ArCP27 in thoracic and abdominal cuticle has the response against the ArCP27 at 5-day old pupa but since then has no response. But the antibody against ArCP27 has reacted to 5- and 7-day old pupal and adult wing protein. $^3H-leucine$ was not incorporated into ArCP27 in 5- and 7-day old thoracic and abdominal cuticle but was incorporated into ArCP27 in 7-day old wing cuticle and adult wing, suggesting that ArCP27 partly participates the wing cuticle formation by the process of digestion and reabsorption of old cuticle.

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