Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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The Effect of Herbal Formula KH-204 on Erectile Dysfunction in Hyperlipidemic Rat

고지혈증 흰쥐에서 생약제제 KH-204의 발기부전 치료연구

  • 이은정 ((주)KMSI 부설 한국의과학연구소) ;
  • 김희석 ((주)KMSI 부설 한국의과학연구소) ;
  • 손동완 (가톨릭대학교 의과대학 비뇨기과학 교실) ;
  • 김세웅 (가톨릭대학교 의과대학 비뇨기과학 교실) ;
  • 조용현 (가톨릭대학교 의과대학 비뇨기과학 교실) ;
  • 황성완 ((주)KMSI 부설 한국의과학연구소) ;
  • 황성연 ((주)KMSI 부설 한국의과학연구소)
  • Published : 2007.03.31
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Abstract

This study was designed to investigate the effects KH-204 on erectile dysfunction in hyperlipidemic rat. KH-204 has been evaluated antihyperlipidemic and antierectile dysfunction effects on experimental hyperlipidemic rats induced by high fat diet. After oral administration of the water extract KH-204 (50, 100, 200, 300 mg/kg) to hyperlipidemic rats for 8 weeks, the variables including body weight, total cholesterol, HDL and LDL levels in serum, the expression of eNOS and nNOS in penis were measured. And erectile function was determined by measurement of intracavernosal pressure (ICP) and maximal arterial pressure (MAP) after electrical stimulation of the cavernosal nerve. Oral administration of KH-204 significantly inhibited the increases of serum total cholesterol and LDL-cholesterol levels and the decreased of serum HDL-cholesterol levels in hyperlipidemic rats induced by high fat diet. The penile expression level of the two enzyme (eNOS, nNOS) were increased significantly after oral administration of the KH-204 50 mg/kg. Erectile function after 10 volts stimulation was significantly decreased in the hyperlipidemic rat compared with the normal rat, but increased in KH-204 group compared with hyperlipidemic group. These results suggest that KH-204 is effective for erectile dysfunction in hyperlipidemia.

Keywords

References

  1. Levy, R. I. (1991) Cholesterol, lipoprotein, approtein and heart disease : present status and future prospects. Clin. Chem. 27: 653-657
  2. 한국과학기술정보연구원 (2004) 발기부전치료제의 시장 동향 분석. 85. 한국과학기술정보 연구원 기술산업 정보분석 보고서. 서울
  3. 고지혈증치료지침제정위원회 (1998) 고지혈증과 동맥경화증. 23. 신광 출판사. 한국
  4. Virag, R., Bouilly, P. and Fryfman, D. (1985) Is impotence an arterial disorder? A study of arterial risk factors in 440 impotent man. Lancet 1: 181-184
  5. Park, K., Son, H., Kim, S. W. and Paick J. S. (2005) Initial validation of a novel rat model of vasculogenic erectile dysfunctionI with generalized atherosclerosis. Int J Impot Res. 17: 424-30 https://doi.org/10.1038/sj.ijir.3901339
  6. Noboru, T., Kazuhede, A. and Tomio, O. (2005) Nitric oxide and penile erectile function. Pharma & Therapeu. 106: 233- 266 https://doi.org/10.1016/j.pharmthera.2004.11.011
  7. 한국생약학교수협의회 (2004) 본초학. 863, 865, 768, 802, 845. 아카데미서적. 한국
  8. Park, C. S., Ryu, S. D. and Hwang, S. Y. (2004) Elevation of intracavernous pressure and NO-cGMP activity by a new herbal in penile tissure of aged and diabetic rats. J Ethnopharmcol. 94: 85-89 https://doi.org/10.1016/j.jep.2004.04.028
  9. Lee, H. J., Lee, E. J., Kim, H. S., Kim S. N. and Hwang S. Y. (2006) Effects of KH-204 on the relaxation response of rabbit corpus cavernosum and reproductive function in male rats. J. Korean Soc. Food Sci. Nutr. 35: 847-852 https://doi.org/10.3746/jkfn.2006.35.7.847
  10. Richmond, W. (1976) Use of cholesterol oxidase for assay of total and free cholesterol in serum by continuous flow analysis. Clin. Chem. 22: 1579-1588
  11. Noma, A., Nakayama, K. N., Kota, M. and Okabe, H. (1978) Simultaneous determination of serum cholesterol in high and low density lipoprotein with use of heparin, $Ca^{2+}$ and an anion exchange resin. Clin. Chem. 24: 1504-1508
  12. Fridewald, W. T., Levy, R. I. and Fedreicson, D. S. (1972) Estimation of concentration of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin. Chem. 18: 499-502
  13. Parmer, R. M., Ferrige, A. G. and Monacada, S. (1987) Nitric oxide release accounts for the biological activity of endothelium derived relaxing factor. Nature 327: 524-526 https://doi.org/10.1038/327524a0
  14. 김수웅 (2005) Erectile dysfunction as a risk marker of cardiovascular risk. 41-49. 제 11차 대한 남성과학회 연구 강좌. 서울
  15. Azadzoi, K. M. and Saenz de Tajade, I. (1991) Hypercholesterolemia imp airs endothelium-dependent relaxation of rabbit corpus cavernosum smooth muscle. J. Urol Res. 146: 238-240 https://doi.org/10.1016/S0022-5347(17)37759-5
  16. Roumeguere, T., Wespes, E., Carpentier, Y., Hoffmann, P. and Schulman, C. C. (2003) Erectile dysfunction is associated with a high prevalence of hyperlipidemia and coponary heartdisease. Eur. Urol. 44: 355-359 https://doi.org/10.1016/S0302-2838(03)00306-3
  17. 노삼현, 최현숙, 이계웅, 이명렬 (2003) 고콜레스테롤혈증 흰쥐에서 산수유의 지질 저하 효과. 한국식품영양과학회 연합학술대회 제 53차
  18. 이향림, 최상원, 최영선, 조성희 (2003) 복분자와 백출메탄올 추출물의 난소절제 흰쥐의 지질대사에 미치는 영향 및 안전성. 한국식품영양과학회 연합학술대회 제 54차
  19. 전영중 (2003) 복분자로부터 분리한 혈당 강하 활성성분의 평가 및 이를 이용한 당뇨 개선 기능성 제품개발. 아미코젠 주식회사 연구보고서. 98
  20. 옥은성 (1995) 오미자 추출물이 고지혈증 흰쥐에 미치는 영향. 한국영양식량학회지. 24: 658-662
  21. 손예건 (1993) 구기자, 구기엽, 지골피가 고혈압, 고지혈증 및 고혈당에 미치는 영향. 경희대 대학원 석사논문