Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antibacterial Activity of Sophoraflavanone G Isolated from the Roots of Sophora flavescens

  • Cha, Jeong-Dan (Institute of Oral Bioscience and Department of Oral Microbiology, School of Dentistry, Chonbuk National University) ;
  • Jeong, Mi-Ran (Research Center of Bioactive Materials, Chonbuk National University) ;
  • Jeong, Seung-Il (Jeonju Biomaterials Institute) ;
  • Lee, Kyung-Yeol (Institute of Oral Bioscience and Department of Oral Microbiology, School of Dentistry, Chonbuk National University)
  • Published : 2007.05.31
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Abstract

This study investigated the antibacterial activities of sophoraflavanone G from Sophora flavescens in combination with two antimicrobial agents against oral bacteria. The combined effect of sophoraflavanone G and the antimicrobial agents was evaluated using the checkerboard method to obtain a fractional inhibitory concentration(FIC) index. The sophoraflavanone G+ampicillin(AM) combination was found to have a synergistic effect against S. mutans, S. sanguinis, S. sobrinus, S. gordonii, A. actinomycetemcomitans, F nucleatum, P. intermedia, and P. gingivalis, whereas the sophoraflavanone G+gentamicin(GM) combination had a synergistic effect against S. sanguinis, S. criceti, S. anginosus, A. actinomycetemcomitans, F nucleatum, P. intermedia, and P. gingivalis. Neither combination exhibited any antagonistic interactions(FIC index>4). In particular, the MICs/MBCs for all the bacteria were reduced to one-half$\sim$one-sixteenth as a result of the drug combinations. A synergistic interaction was also confirmed by time-kill studies for nine bacteria where the checkerboard suggested synergy. Thus, a strong bactericidal effect was exerted through the drug combinations, plus in vitro data suggested that sophoraflavanone G combined with other antibiotics may be microbiologically beneficial rather than antagonistic.

Keywords

References

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