Antioxidant Activity of Sulfated Polysaccharides Isolated from Sargassum fulvellum

  • Choi, Dae-Sung (Biomaterials Science and Engineering Laboratory, Massachusetts Institute of Technology) ;
  • Athukorala, Yasantha (Faculty of Applied Marine Science, Cheju National University) ;
  • Jeon, You-Jin (Faculty of Applied Marine Science, Cheju National University) ;
  • Senevirathne, Mahinda (Department of Food Bioengineering, Cheju National University) ;
  • Cho, Kyun-Rha (Biomaterials Science and Engineering Laboratory, Massachusetts Institute of Technology) ;
  • Kim, Soo-Hyun (Department of Food Bioengineering, Cheju National University, Biomaterials Science and Engineering Laboratory, Massachusetts Institute of Technology)
  • Published : 2007.06.30


Sargassum fulvellum, a marine brown alga, is a popular low priced edible plant in Korean markets. The polysaccharide fraction of the alga was separated and investigated for its radical scavenging activities and the results compared with those of commercial fucoidans (Fucus vesiculosus and Undaria pinnatifida), BHA and ${\alpha}$-tocopherol. The polysaccharide fraction of S. fulvellum showed a promising DPPH radical scavenging activity than did other fucoidans. Moreover, the sample exhibited a dose-dependent activity on hydrogen peroxide scavenging activity in the V79-4 cell line. Interestingly, all the tested polysaccharide counterparts were more potent NO. scavengers than were the commercial antioxidants, BHA and ${\alpha}$-tocopherol. The sulfated polysaccharide of S. fulvellum had an approximate molecular weight of 529 kDa and mainly consisted of fucose and galactose, and minor amounts of mannose, rhamnose and xylose.


  1. Cannel RJP. 1993. Algae as a source of biologically active products. Pestic Sci 39: 147-153
  2. Raghavendrran HB, Sathivel A, Yogeeta RS, Devaki T. 2007. Efficacy of Sargassum polycystum sulphated polysacchride against paracetamol-induced DNA fragmentation and modulation of membrane-bound phosphatases during toxic hepatitis. Clin Exp Pharmacol Physiol 34: 142-147
  3. Choi JH, Pyeun JH, Rhim CH, Yang JS, Kim SH, Kim JH, Lee BH, Woo SI, Choi SN, Byun DS. 1986. In-vestigation of daily life and consciousness of longevous people in Korea. Korean J Dietary Culture 1: 116-126
  4. Matsumoto S, Nagaoka M, Hara T, Kimura-Takagi I, Mistuyama K, Ueyama S. 2004. Fucoidan derived from Cladosiphon okamuranus Tokida ameliorates murine chronic colitis through the down-regulation of interlukin-6 prodution on colonic epithelial cells. Clin Exp Immunol 136: 432-439
  5. Nagaoka M, Shibata H, Kimura-Takagi I, Hashimoto S, Kimura K, Makino T, Aiyama R, Ueyama S, Yokokura T. 1999. Structural study of fucoidan from Cladosiphon okamuranus Tokida. Glycoconj J 16: 19-26
  6. Haneji K, Matsuda T, Tomita M, Kawakami H, Ohshiro K, Uchihara JN, Masuda M, Takasu N, Tanaka Y, Ohta T, Mori N. 2005. Fucoidan extracted from Cladosiphon okamuranus Tokida induces apoptosis of human T-cell leukemia virus type 1-infected T-cell lines and primary adult T-cell leukemia cells. Nutr Cancer 52: 189-201
  7. Matsumoto S, Nagaoka M, Hara T, Kimura-Takagi I, Mistuyama K, Ueyama S. 2004. Fucoidan derived from Cladosiphon okamuranus Tokida ameliorates murine chronic colitis through the down-regulation of interleukin-6 production on colonic epithelial cells. Clin Exp Immunol 36: 432-439
  8. Terasaki M, Itabashi Y. 2003. Glycerolipid acyl hydrolase activity in the brown alga Cladosiphon okamuranus TOKIDA. Biosci Biotechnol Biochem 67: 1986-1989
  9. Koo JG, Jo KS, Do JR, Woo SJ. 1995. Isolation and purification of fucoidans from Laminaria religiosa and Undaria Pinnatifida in Korea. J Korean Fish Soc 28: 227-236
  10. Duarte MER, Cardoso MA, Noseda MD, Cerezo AS. 2001. Structural studies on fucoidans from the brown seaweeds Sargassum stenophyllum. Carbo Res 333: 281-293
  11. Brand-Williams W. 1985. Use of a free radical method to evaluate antioxidant activity. Food Sci Tech 28: 25-30
  12. Nagai T, Sakai M, Inoue R, Inoue H, Suzuki N. 2001. Antioxidative activities of some commercially honeys, royal jelly, and propolis. Food Chem 75: 237-240
  13. Muller HE. 1985. Detection of hydrogen peroxide produced by microorganism on ABTS-peroxidase medium. Zentralbl Bakteriol Mikrobiol Hyg [A] 259: 151-154
  14. Chung SK, Osawa T, Kawakishi S. 1997. Hydroxyl radical scavenging effects of spices and scavengers from brown mustard (Brassica nigra). Biosci Biotech Biochem 61: 118-123
  15. Garrat DC. 1964. The Quantitative Analysis of Drugs. Chapman and Hall, Tokyo, Japan. Vol 3, p 456-458
  16. Chandler SF, Dodds JH. 1993. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum laciniatum. Plant Cell Rep 2: 105-110
  17. Athukorala Y, Jung WK, Vasanthan T, Jeon YJ. 2006. An anticoagulative polysaccharide from an enzymatic hydrolysate of Ecklonia cava. Carbohydr Polym 66: 184-191
  18. Shon MY, Kim TH, Sung NJ. 2003. Antioxidant and free radical scavenging activity Phellinus baumii (Phellinus of Hymenochaetaceae) extracts. Food Chem 82: 593-597
  19. Li XL, Zhou AG, Han Y. 2006. Anti-oxidation and anti-microorganism activities of purification polysaccharide from Lygodium japonicum in vitro. Carbohydr Polym 66: 34-42
  20. Zhang Q, Yu P, Li Z, Zhang H, Xu Z, Li P. 2003. Antioxidant activities of sulfated polysaccharide fractions from Porphyra haitanesis. J Appl Phycol 15: 305-310
  21. Zhao X, Xue CH, Li ZJ, Cai YP. 2004. Antioxidant and hepatoprotective activities of low molecular weight sulfated polysaccharide from Laminaria japonica. J Appl Phycol 16: 111-115
  22. Qi H, Zhao T, Zhang Q, Li Z, Zhao Z, Xing R. 2005. Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm (Chlorophyta). J Appl Phycol 17: 527-534
  23. Halliwell B. 1991. Reactive oxygen species in living systems: source, biochemistry, and role in human disease. Am J Med 91: 14-22
  24. Athukorala Y, Kim KN, Jeon YJ. 2006. Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food Chem Toxicol 44: 1065-1074
  25. Subramanian M, Chintalwar GJ, Chattopadhyay S. 2002. Antioxidant properties of a Tinospora cordifolia polysaccharide against iron-mediated lipid damage and ${\gamma}-ray$ induced protein damage. Redox Report 7: 137-143
  26. Senevirathne M, Kim SH, Siriwardhana N, Ha JH, Lee KW, Jeon YJ. 2006. Antioxidant potential of Ecklonia cava on reactive oxygen species scavenging, metal chelating, reducing power and lipid peroxidation inhibition. Food Sci Tech Int 12: 27-38
  27. Mansouri A, Makris D, Kefalas P. 2005. Determination of hydrogen peroxide scavenging activity of cinnamic and benzoic acids employing a highly sensitive peroxyoxalate chemiluminescence-based assay: structure-activity rela-tionships. J Pharm Biomed Anal 39: 22-26
  28. Hu JF, Geng MY, Zhang JT, Jiang HD. 2001. An in vitro study of the structure-activity relationships of sulfated polysaccharide from brown algae to its antioxidant effect. J Asian Nat Prod Res 3: 353-358
  29. Nergard CS, Diallo D, Inngjerdingen K, Michaelsen TE, Matsumoto T, Kiyohara H, Yamada H, Paulsen BS. 2005. Medicinal use of Cochlospermum tinctorium in Mali anti-ulcer-, radical scavenging and immunomodulating activities of polymers in the aqueous extract of the roots. J Ethanopharmaco 96: 255-269
  30. Dawczynski C, Schubert R, Jahreis G. 2006. Amino acids, fatty acids, and dietary fibre in edible seaweed products. Food Chem (doi:10.1016/j.foodchem.2006.09.041)
  31. Li XM, Li XL, Zhou AG. 2006. Evaluation of antioxidant activity of the polysaccharides extracted from Lycium barbarum fruits in vitro. Euro Polym J doi:10.1016/j. eurpolymj.10.025
  32. Kishk YFM, Al-Sayed HMA. 2007. Free-radical scaveng-ing and antioxidative activities of some polysaccharides in emulsions. LWT-Food Sci Technol 40: 270-277
  33. Liu F, Ooi VEC, Chang ST. 1997. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 60: 763-771
  34. Sun C, Wang JW, Fang L, Gao XD, Tan RX. 2004. Free radical scavenging and antioxidant activities of EPS2, an exopolysaccharide produced by a marine filamentous fungus Keissleriella sp. YS 4108. Life Sci 75: 1063-1073
  35. Kolb N, Vallorani L, Stocchi V. 1999. Chemical composition and evaluation of protein quality by amino acid score method of edible brown marine algae Arame (Eisenia bicyclis) and Hijiki (Hijikia fusiforme). Acta Alimentaria 28: 213-222
  36. Van den Hoek C, Jahns HM, Mann DG. 1993. Algen. In Neubearbeitete Auflage. 3rd ed. Georg Thieme Verlag, Stuttgart, New York. p 12-13, 42, 135-136
  37. Scrig AJ, Muniz FJS. 2000. Dietary fiber from edible seaweeds: chemical structure, physiological properties and effects on the cholesterol metabolism. Nutr Res 20: 585-598

Cited by

  1. Characterization and antioxidant activities of degraded polysaccharides from two marine Chrysophyta vol.160, 2014,
  2. Antioxidant properties of sequential extracts from brown seaweed, Sargassum plagiophyllum, C. Agardh vol.2, 2012,
  3. Purification, Structural Characterization, and Antioxidant Activity of Antioxidant Substance from the Red SeaweedGloiopeltis tenax vol.12, pp.2, 2009,
  4. Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae vol.84, pp.1, 2011,
  5. Cytoprotective effect of fucoxanthin isolated from brown algae Sargassum siliquastrum against H2O2-induced cell damage vol.228, pp.1, 2008,
  6. Fibrinolytic Compounds Isolated from a Brown Alga, Sargassum fulvellum vol.7, pp.2, 2009,
  7. Sulfated polysaccharides as bioactive agents from marine algae vol.62, 2013,
  8. Role of the NO/KATP pathway in the protective effect of a sulfated-polysaccharide fraction from the algae Hypnea musciformis against ethanol-induced gastric damage in mice vol.23, pp.2, 2013,
  9. Effect of enzyme-assisted extract of Sargassum coreanum on induction of apoptosis in HL-60 tumor cells vol.24, pp.4, 2012,
  10. Biological Analysis of Enzymatic Extracts from Sargassum fulvellum Using Polysaccharide Degrading Enzyme vol.28, pp.6, 2013,
  11. Natural antioxidants from sea: a potential industrial perspective in aquafeed formulation 2016,
  12. Proteomic analysis of scallop hepatopancreatic extract provides insights into marine polysaccharide digestion vol.6, pp.1, 2016,
  13. Angiotensin Converting Enzyme Inhibitory, Antioxidant Activities, and Antihyperlipidaemic Activities of Protein Hydrolysates From Scallop Mantle (Chlamys Farreri) vol.18, pp.1, 2015,
  14. Marine Polysaccharides from Algae with Potential Biomedical Applications vol.13, pp.5, 2015,
  15. Bioactive polysaccharides from marine algae vol.4, pp.2, 2014,
  16. Towards a better understanding of medicinal uses of the brown seaweed Sargassum in Traditional Chinese Medicine: A phytochemical and pharmacological review vol.142, pp.3, 2012,
  17. Fucans from a Tunisian brown seaweed Cystoseira barbata: Structural characteristics and antioxidant activity vol.66, 2014,
  18. α-Glucosidase Inhibitory Effects for Solvent Fractions from Methanol Extracts of Sargassum fulvellum and Its Antioxidant and Alcohol-Metabolizing Activities vol.22, pp.10, 2012,
  19. Recent developments in the application of seaweeds or seaweed extracts as a means for enhancing the safety and quality attributes of foods vol.12, pp.4, 2011,
  20. Marine food-derived functional ingredients as potential antioxidants in the food industry: An overview vol.44, pp.2, 2011,
  21. Chemical characterization and antioxidant activity of sulfated polysaccharide from the red seaweed Gracilaria birdiae vol.27, pp.2, 2012,
  22. to dietary fucoidan vol.46, pp.1, 2018,
  23. and their radical scavenging, metal chelating, and cholinesterase inhibitory activities vol.22, pp.1, 2019,