Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Fucoidan on Neuronal Cell Proliferation: Association with NO Production through the iNOS Pathway

  • Lee, Hye-Rim (College of Pharmacy, Sungkyunkwan University) ;
  • Do, Hang (College of Pharmacy, Sungkyunkwan University) ;
  • Lee, Sung-Ryul (College of Pharmacy, Sungkyunkwan University) ;
  • Sohn, Eun-Soo (Information Analysis Center, KISTI) ;
  • Pyo, Suhk-Neung (College of Pharmacy, Sungkyunkwan University) ;
  • Son, Eun-Wha (Institute of Bioscience and Biotechnology, Kangwon National University)
  • Published : 2007.06.30
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Abstract

Fucoidan, that is high-molecular-weight sulfated polysaccharides extracted from brown seaweeds has been shown to elicit various biological activities. Here, we investigated the effects of fucoidan on cell proliferation and nitric oxide (NO) production in neuronal blastoma cell (SH-SY5Y). In the present study, we demonstrated that fucoidan treatment resulted in increase of cell proliferation and NO production. When cells were treated with amyloid-${\beta}$ (A${\beta}$) in the absence or presence of fucoidan, fucoidan recovered the cell viability decreased by A${\beta}$ peptides. To further determine whether nitric oxide synthase (NOS) is involved in proliferative effect of fucoidan, cells were treated with NOS inhibitors in the absence or presence of fucoidan. Selective constitutive nitric oxide synthase (cNOS) inhibitor, diphenylene iodonium chloride (DPI), caused a decrease of cell viability, whereas cell viability was increased by specific inducible nitric oxide synthase (iNOS) inhibitor, S-methylisothiourea (SMT), in the fucoidan-untreated cells. Treatment with fucoidan inhibited the cell viability decreased in DPI-exposed cells. In contrast, fucoidan had no effect on cell growth in SMT-treated cells, indicating that cNOS may not play a role in the proliferation of fucoidan-treated cells. The present data suggest that fucoidan has proliferative and neuroprotective effects and these effects may be associated with iNOS.

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References

  1. Davis TA, Volesky B, Mucci A. 2003. A review of the biochemistry of heavy metal biosorption by brown algae. Water Res 37: 4311-4330 https://doi.org/10.1016/S0043-1354(03)00293-8
  2. Patel MK, Mulloy B, Gallagher KL, O'Brien L, Hughes AD. 2002. The antimitogenic action of the sulphated polysaccharide fucoidan differs from heparin in human vascular smooth muscle cells. Thromb Haemost 87: 149-154 https://doi.org/10.1055/s-0037-1612958
  3. O'Leary R, Rerek M, Wood EJ. 2004. Fucoidan modulates the effect of transforming growth factor (TGF)-beta1 on fibroblast proliferation and wound repopulation in vitro models of dermal wound repair. Biol Pharm Bull 27: 266-270 https://doi.org/10.1248/bpb.27.266
  4. Mahony MC, Oehninger S, Clark GF, Acosta AA, Hodgen GD. 1991. Fucoidin inhibits the zona pellucida-induced acrosome reaction in human spermatozoa. Contraception 44: 657-665 https://doi.org/10.1016/0010-7824(91)90085-T
  5. Baba M, Snoeck R, Pauwels R, de Clercq E. 1988. Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes simplex virus, cytomegalovirus, vesicular stomatitis virus, and human immunodeficiency virus. Antimicrob Agents Chemother 32: 1742-1745 https://doi.org/10.1128/AAC.32.11.1742
  6. Ooi VE, Liu F. 2000. Immunomodulation and anti-cancer activity of polysaccaride-protein complexes. Curr Med Chem 7: 715-729 https://doi.org/10.2174/0929867003374705
  7. Bredt DS, Snyder SH. 1994. Nitric oxide: a physiologic messenger molecule. Annu Rev Biochem 63: 175-195 https://doi.org/10.1146/annurev.bi.63.070194.001135
  8. Yun HY, Dawson VL, Dawson TM. 1997. Nitric oxide in health and disease of the nervous system. Mol Psychiatry 2: 300-310 https://doi.org/10.1038/sj.mp.4000272
  9. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Methods 65: 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  10. Sladowski D, Steer SJ, Clothier RH, Balls M. 1993. An improved MTT assay. J Immunol Methods 157: 203-207 https://doi.org/10.1016/0022-1759(93)90088-O
  11. Roehm NW, Rodgers GH, Hatfield SM, Glasebrook AL. 1991. An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J Immunol Methods 142: 257-265 https://doi.org/10.1016/0022-1759(91)90114-U
  12. Ding AH, Nathan CF, Stuer DJ. 1988. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J Immunol 141: 2407-2412
  13. Giraux JL, Matou S, Bros A, Tapon-Bretaudiere J, Letourneur D, Fischer AM. 1998. Modulation of human endothelial cell proliferation and migration by fucoidan and heparin. Eur J Cell Biol 77: 352-359 https://doi.org/10.1016/S0171-9335(98)80094-0
  14. Religa P, Kazi M, Thyberg J, Gaciong Z, Swedenborg J, Hedin U. 2000. Fucoidan inhibits smooth muscle cell proliferation and reduces mitogen-activated protein kinase activity. Eur J Vasc Endovasc Surg 20: 419-426 https://doi.org/10.1053/ejvs.2000.1220
  15. Yang WW, Krukoff TL. 2000. Nitric oxide regulates body temperature, neuronal activation and interleukin-1$\beta$ gene expression in the hypothalamic paraventricular nucleus in response to immune stress. Neuropharmacology 39: 2075- 2089 https://doi.org/10.1016/S0028-3908(00)00054-X
  16. Yun Xia, Krukoff TL. 2004. Estrogen induces nitric oxide production via activation of constitutive nitric oxide synthases in human neuroblastoma cells. Endocrinology 145: 4550-4557 https://doi.org/10.1210/en.2004-0327
  17. Pesaresi M, Lovati C, Bertora P, Mailland E, Galimberti D, Scarpini E, Quadri P, Forloni G, Mariani C. 2006. Plasma levels of beta-amyloid (1-42) in Alzheimer's disease and mild cognitive impairment. Neurobiol Aging 27: 904-905 https://doi.org/10.1016/j.neurobiolaging.2006.03.004
  18. Bonfoco E, Krainc D, Ankarcrona M, Nicotera P, Lipton SA. 1995. Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. Proc Natl Acad Sci USA 92: 7162-7166 https://doi.org/10.1073/pnas.92.16.7162
  19. Nelson RJ, Kriegsfeld LJ, Dawson VL, Dawson TM. 1997. Effects of nitric oxide on neuroendocrine function and behavior. Front Neuroendocrinol 18: 463-491 https://doi.org/10.1006/frne.1997.0156
  20. Yermolaieva O, Brot N, Weissbach H, Heinemann SH, Hoshi T. 2000. Reactive oxygen species and nitric oxide mediate plasticity of neuronal calcium signaling. Proc Natl Acad Sci USA 97: 448-453 https://doi.org/10.1073/pnas.97.1.448
  21. Stojkovic T, Colin C, Le Saux F, Jacque C. 1998. Specific pattern of nitric oxide synthase expression in glial cells after hippocampal injury. Glia 22: 329-337 https://doi.org/10.1002/(SICI)1098-1136(199804)22:4<329::AID-GLIA2>3.0.CO;2-Z
  22. Demas GE, Eliasson MJ, Dawson TM, Dawson VL, Kriegsfeld LJ, Nelson RJ, Snyder SH. 1997. Inhibition of neuronal nitric oxide synthase increases aggressive behavior in mice. Mol Med 3: 610-616
  23. Kriegsfeld LJ, Demas GE, Huang PL, Burnett AL, Nelson RJ. 1999. Ejaculatory abnormalities in mice lacking the gene for endothelial nitric oxide synthase (eNOS-/-). Physiol Behav 67: 561-566 https://doi.org/10.1016/S0031-9384(99)00100-6
  24. Yolken RH, Karlsson H, Yee F, Johnston-Wilson NL, Torrey EF. 2000. Endogenous retroviruses and schizo-phrenia. Brain Res Rev 31: 193-199 https://doi.org/10.1016/S0165-0173(99)00037-5
  25. Jarskog LF, Gilmore JH, Selinger ES, Lieberman JA. 2000. Cortical bcl-2 protein expression and apoptotic regulation in schizophrenia. Biol Psychiatry 48: 641-650 https://doi.org/10.1016/S0006-3223(00)00988-4

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