The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Inoculum Sources to Generate High Mechanical Transmission of Barley yellow mosaic virus

  • Jonson, Gilda (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Kim, Yang-Kil (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Kim, Mi-Jung (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Park, Jong-Chul (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Hyun, Jong-Nae (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Kim, Jung-Gon (Honam Agricultural Research Institute, National Institute of Crop Science, RDA)
  • Published : 2007.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Mechanical transmission of barley seedlings with barley yellow mosaic virus (BaYMV) is generally inefficient and is the major constraint for testing cultivar resistance to the virus. To explore mechanical transmission, BaYMV-infected barley plants were grown at different conditions and used as inoculum sources to seedlings of susceptible barley cultivar Baegdong. Extracts prepared from BaYMV-infected Baegdong plants at 47, 53, 74, and 90 days after symptom appearance (DASA) and grown at 10 and $12^{\circ}C$ gave 10, 30, 68 and 76% infection, respectively on inoculated susceptible barley cv. Baegdong seedlings. While Jinyangbori, another susceptible cultivar obtained 95% infection rate inoculated with extracts from 90 DASA disease source and grown at $10/12^{\circ}C$. However, low infection rates were obtained when the virus sources were grown in a greenhouse at $15-18^{\circ}C$. Our results indicate that longer incubation period and lower temperature are required for virus accumulation and stability.

Keywords

References

  1. Adams, M. J. 2000. Barley yellow mosaic virus. In: Description of Plant Viruses, No. 374. Association of Applied Biologist, UK
  2. Freidt, W. 1983. Mechanical transmission of soil-borne barley yellow mosaic virus. Phytopath. Z. 106: 16-22 https://doi.org/10.1111/j.1439-0434.1983.tb00023.x
  3. Huth, W. 1991. Barley yellow mosaic-a disease in Europe caused by two different viruses. In: Viruses with fungal vectors, ed. by J. I. Cooper and M. J. Asher. pp. 61-70. Association of Applied Biologist, UK
  4. Hyun, J. N., Han, S. I., Kim, H. T., Kang, J. R., Ko, J. M., Lim, S. G, Kwon, Y. C., Park, D. S., Suh, D. Y., Kim, D. H., Yoon, C. H., Kim, S. T., Suh, S. J. and Kim, S. C. 2006 A new barley cultivar, 'Hopum' with high quality and BaYMV resistance. Korean J. Breed. 38:67-68
  5. Jonson, G, Park, J. C., Noh, T. W., Kim, M. J., Hyun, J. N. and Kim, J. G. 2006. Development of an efficient mechanical inoculation technique to screen barley genotypes for resistance to Barley mild mosaic virus disease and its comparison to natural infection. Plant Pathol. J. 22:348-352 https://doi.org/10.5423/PPJ.2006.22.4.348
  6. Kanyuka, K., Ward, E. and Adams, M. J. 2003. Polymyxa graminis and the cereal viruses it transmits: a research challenge. Mol. Plant Pathol. 4:393-406 https://doi.org/10.1046/j.1364-3703.2003.00177.x
  7. Kashiwazaki, S., Ogawa, K., Usugi, T., Omura, T. and Tsuchizaki, T. 1989. Characterization of several strains of barley yellow mosaic virus. Ann. Phytopath. Soc. Japan. 55:16-25 https://doi.org/10.3186/jjphytopath.55.16
  8. Lee, J. L. 1998. Analysis and detection of coat protein gene of barley yellow mosaic virus and barley mild mosaic virus by RT-PCR. Korean J. Plant Pathol. 14:314-318
  9. Takanashi, K., Saito, Y. and Iwata, Y. 1967 Enzyme inhibitors added to the inoculum in mechanical inoculation of soil-borne viruses of wheat and barley. Ann. Phytopathol Jpn. 33:43-47 https://doi.org/10.3186/jjphytopath.33.43
  10. Kuntze, L., Bauer, E. and Foroughhi-Wehr, B. 2000. An improved method for the inoculation and detection of Ba YMV-2 in winter barley. J. Plant Dis. Prot. 107:310-317
  11. Park, J. C., Han, O. K., Hyun, J. N., Kim, M. J. and Kim, J. G. 2005. Degree of field reduction by viral disease following by different resistance. Korean J. Crop Sci. 50: 178-179
  12. Slykhuis, J. T. 1974. Factors critical to mechanical transmissibility of wheat spindle streak mosaic virus. Phytopathology 65:582-584 https://doi.org/10.1094/Phyto-65-582
  13. So, I. Y., Lee, K. J., Chon, K. H. and Seo, J. H. 1997. Distribution and screening for barley cultivars resistance to barley yellow mosaic virus and barley mild mosaic virus in Southern Korea. Korean J. Plant Pathol. 13:118-124