Journal of Biosystems Engineering

Korean Society for Agricultural Machinery (한국농업기계학회)
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Development of Culture System for Masspropagation and Acclimatization of Tissue Cultured Plantlets

유식물체 증식.순화용 배양시스템 개발

  • Han, K.S. (National Institute of Agricultural Engineering, RDA) ;
  • Heo, J.W. (National Institute of Agricultural Engineering, RDA) ;
  • Kim, S.C. (National Institute of Agricultural Engineering, RDA) ;
  • Lee, Y.B. (National Institute of Agricultural Engineering, RDA) ;
  • Kim, S.C. (National Institute of Agricultural Engineering, RDA) ;
  • Im, D.H. (National Institute of Agricultural Engineering, RDA) ;
  • Choi, H.G. (National Institute of Agricultural Engineering, RDA)
  • Published : 2007.04.25
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Abstract

In mass production of seed-potato plantlets, the processes for in vitro propagation and ex vitro acclimatization with a high cost should be improved by a culture system with environmental control using scaled-up culture vessels. The experiment was conducted to design a hydroponic culture system for enhancement of growth and development of seed-potato (Solanum tuberosum) plantlets cultured under photoautotrophic (without sugar in culture medium) conditions with controlled light intensity and ventilation rate. The culture system was consisted of scaled-up culture vessels, ventilation pipes, a multi-cell tray and an environmental control system (ECS) for optimum controlling in temperature, light intensity, ventilation rate, and culture-medium supply. Growth and development of the plantlets was significantly increased under the ECS compared with a conventional culture system (CCS) of photomixotrophic culture (with sugar in culture medium) using small scale vessels. For 21 days, leaf area of the plantlets was expanded more than 2 times, and number of internodes also approximately 4 times greate. under the ECS. In addition, the photoautotrophic growth in sweetpotato (Ipomoea batatas) and chrysanthemum (Chrysanthemum morifolium) plantlets was greater more than 2 times compared with the CCS.

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References

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