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유식물체 증식.순화용 배양시스템 개발

Development of Culture System for Masspropagation and Acclimatization of Tissue Cultured Plantlets

  • Han, K.S. (National Institute of Agricultural Engineering, RDA) ;
  • Heo, J.W. (National Institute of Agricultural Engineering, RDA) ;
  • Kim, S.C. (National Institute of Agricultural Engineering, RDA) ;
  • Lee, Y.B. (National Institute of Agricultural Engineering, RDA) ;
  • Kim, S.C. (National Institute of Agricultural Engineering, RDA) ;
  • Im, D.H. (National Institute of Agricultural Engineering, RDA) ;
  • Choi, H.G. (National Institute of Agricultural Engineering, RDA)
  • 발행 : 2007.04.25

초록

In mass production of seed-potato plantlets, the processes for in vitro propagation and ex vitro acclimatization with a high cost should be improved by a culture system with environmental control using scaled-up culture vessels. The experiment was conducted to design a hydroponic culture system for enhancement of growth and development of seed-potato (Solanum tuberosum) plantlets cultured under photoautotrophic (without sugar in culture medium) conditions with controlled light intensity and ventilation rate. The culture system was consisted of scaled-up culture vessels, ventilation pipes, a multi-cell tray and an environmental control system (ECS) for optimum controlling in temperature, light intensity, ventilation rate, and culture-medium supply. Growth and development of the plantlets was significantly increased under the ECS compared with a conventional culture system (CCS) of photomixotrophic culture (with sugar in culture medium) using small scale vessels. For 21 days, leaf area of the plantlets was expanded more than 2 times, and number of internodes also approximately 4 times greate. under the ECS. In addition, the photoautotrophic growth in sweetpotato (Ipomoea batatas) and chrysanthemum (Chrysanthemum morifolium) plantlets was greater more than 2 times compared with the CCS.

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참고문헌

  1. Heo, J. W. and T. Kozai. 1999. Forced ventilation micro-propagation system for enhancing photosynthesis, growth, and development of sweetpotato plantlets. Environ. Control in Biol. 37(1):83-92 https://doi.org/10.2525/ecb1963.37.83
  2. Heo, J. W., S. B. Wilson, T. Kozai. 2001. A forced ventilation micropropagation system for photoautotrophic production of sweetpotato plug plantlets in a scaled-up culture vessel : 1. Growth and uniformity. Horttechnology 11(1):90-94
  3. Kim, S. J., E. J. Hahn, J. W. Heo, K. Y. Paek. 2004. Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci. Horticulturae 101:143-151 https://doi.org/10.1016/j.scienta.2003.10.003
  4. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  5. Nakayama, M., T. Kozai, K. Watanabe. 1991. Effects of the presence/absence of sugar in the medium and natural/forced ventilation on the net photosynthetix rates of potato explants in vitro. Plant Tiss. Cult. Lett. 8:105-109
  6. Nguyen, Q. T., T. Kozai, J. W. Heo, D. X. Thai. 2001. Photoautotrophic growth response of in vitro cultured coffee plantlets to ventilation methods and photosynthetix photon fluxes under carbon dioxide enriched conditions. Plant Cell Tiss. & Org. Cult. 66:217-225 https://doi.org/10.1023/A:1010662413486
  7. Shim, S. W., J. W. Heo, S. K. Kim, K. Y. Paek. 2001. Effects of number of air exchanges, sucrose and BA on shoot proliferation and growth of 'Campbell Early' grape (Vitis hybrid) plantlets. J. KSHS. 42(5):540-544
  8. Wilson, S. B., J. W. Heo, C. Kubota, T. Kozai. 2001. A Forced ventilation micropropagation System for photoautotrophic production of sweetpotato plug plantlets in a scaled-up culture vessel: 2. Carbohydrate status. HortTechnology 11(1):95-99