Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning of Fibrinolytic Enzyme Gene from Bacillus subtilis Isolated from Cheonggukjang and Its Expression in Protease-deficient Bacillus subtilis Strains

  • Jeong, Seon-Ju (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Kwon, Gun-Hee (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Chun, Ji-Yeon (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Kim, Jong-Sang (Department of Animal Biotechnology, Kyungpook National University) ;
  • Park, Cheon-Seok (Department of Food Science & Biotechnology, Kyung Hee University) ;
  • Kwon, Dae-Young (Food Function Research Division, Korea Food Research Institute) ;
  • Kim, Jeong-Hwan (Institute of Agriculture & Life Science, Gyeongsang National University)
  • Published : 2007.06.30
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Abstract

Bacillus subtilis CH3-5 was isolated from cheonggukjang prepared according to traditional methods. CH3-5 secreted at least four different fibrinolytic proteases (63, 47, 29, and 20 kDa) into the culture medium. A fibrinolytic enzyme gene, aprE2, encoding a 29kDa enzyme was cloned from the genomic DNA of CH3-5, and the DNA sequence determined. aprE2 was overexpressed in heterologous B. subtilis strains deficient in extracellular proteases using a E. coli-Bacillus shuttle vector. A 29 kDa AprE2 band was observed and AprE2 seemed to exhibit higher activities towards fibrin rather than casein.

Keywords

References

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