Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Sources and Distributions of Dissolved Organic Matter by Fluorescence Method in the Northeastern Pacific Ocean

북동태평양에서 형광 기법을 이용한 용존유기물의 기원 및 분포

  • 손주원 (한국해양연구원 해양자원연구본부) ;
  • 손승규 (한국해양연구원 해양자원연구본부) ;
  • 주세종 (한국해양연구원 해양자원연구본부) ;
  • 김경홍 (한국해양연구원 해양자원연구본부) ;
  • 김웅서 (한국해양연구원 해양자원연구본부) ;
  • 박용철 (인하대학교 자연과학대학 해양과학과)
  • Published : 2007.06.30
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Abstract

This study was conducted to understand the source and behavior of organic matter using the fluorescent technique (excitation-emission matrix) as a part of environmental monitoring program in the Korea manganese nodule mining site in the Northeastern Pacific Ocean. Water samples were collected at $0^{\circ},\;6^{\circ}N$, and $10.5^{\circ}N$ along $131.5^{\circ}W$ in August 2005. The concentration of total organic carbon (TOC) ranged from 58.01 to $171.93\;{\mu}M-C$. The vertical distribution of TOC was characterized as higher in the surface layer and decreased with depth. At $6^{\circ}N$, depth-integrated (from surface to 200 m depth) TOC was $337.1\;gC/m^2$, which was 1.4 times higher value than other stations. The exponential decay curve fit of vertical profile of TOC indicated that 59% of organic carbon produced by primary production in the surface layer could be decomposed by bacteria in the water column. Dissolved organic matter is generally classified into two distinctive groups based on their fluorescence characteristics using three-dimensional excitation/emission (Ex/Em) fluorescence mapping technique. One is known as biomacromolecule (BM; protein-like substance; showing max. at Ex 280/Em 330), mainly originated from biological metabolism. The other is geomacromolecule (GM; humic-like substance; showing max. at Ex 330/Em 430), mainly originated from microbial degradation processes. The concentration of BM and GM was from 0.42 to 7.29 TU (tryptophan unit) and from 0.06 to 1.81 QSU (quinine sulfate unit), respectively. The vertical distribution of BM was similar to that of TOC as high in the surface and decreased with depth. However, the vertical distribution of GM showed the reverse pattern of that of BM. From these results, it appeared that BM occupied a major part of TOC and was rapidly consumed by bacteria in the surface layer. GM was mainly transformed from BM by microbial processes and was a dominant component of TOC in the deep-sea layer.

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References

  1. 손승규, 박용철. 1997. 북동태평양(KODOS 해역)의 영양염및 형광 유기물에 관한 환경특성 연구. 한국환경과학회지, 6, 595-604
  2. 손주원, 손승규, 김경홍, 김기현, 박용철, 김동화, 김태하. 2005. 북동태평양 KODOS 해역의 유기탄소 및 겉보기 산소량 특성. Ocean and Polar Res., 27, 1-13
  3. 현정호, 김경홍, 권개경, 이정현, 이홍금, 김상진, 김기현. 2002. 아데노신 3인산을 이용한 심해저 및 연안퇴적토의 총 미생물 생체량 측정. Kor. J. Microbiol., 38, 119-126
  4. 해양수산부. 1998. 심해저 광물자원탐사 보고서 제1권, p. 998-1000
  5. 해양수산부. 2006. 심해저 광물자원개발 보고서 제1권, p. 100-172
  6. Anderson, T.R. and P.J.le.B. Williams. 1999. A onedimensional model of dissolved organic carbon cycling in the water column incorporating combined biologicalphotochemical decomposition. Global Biogeochem. Cycles, 13, 337-349 https://doi.org/10.1029/1999GB900013
  7. Chen, R.F. and J.L. Bada. 1992. The fluorescence of dissolved organic matter in seawater. Mar. Chem., 37, 191-221 https://doi.org/10.1016/0304-4203(92)90078-O
  8. Coble, P.G., C.A. Schultz, and K. Mopper. 1993. Fluorescence contouring analysis of DOC intercalibration experiment samples. Mar. Chem., 41, 173-178 https://doi.org/10.1016/0304-4203(93)90116-6
  9. Coble, P.G., R.G. Zepp, and R.G. Zika. 2004. CDOM in the ocean: Characterization, distribution and transformation ocean sciences meeting, Honolulu, HI, 11-15 Feb. 2002. Mar. Chem., 89, 3-327 https://doi.org/10.1016/j.marchem.2004.03.019
  10. Determann, S., J.M. Lobbes, R. Reuter, and J. Rullkotter. 1998. Ultraviolet fluorescence excitation and emission spectroscopy of marine algae and bacteria. Mar. Chem., 62, 137-156 https://doi.org/10.1016/S0304-4203(98)00026-7
  11. Druffel, E.R.M., P.M. Williams, and Y. Suzuki. 1989a. Concentrations and radiocarbon signatures of dissolved organic matter in the Pacific Ocean. Geophys. Res. Lett., 16, 991-994 https://doi.org/10.1029/GL016i009p00991
  12. Druffel, E.R.M., P.M. Williams, K. Robertson, S. Griffin, A.J.T. Jull, D. Donahue, L. Toolin, and T.W. Linick. 1989b. Radiocarbon in dissolved organic and inorganic carbon from the Central North Pacific. Radiocarbon, 31, 523-532
  13. Hanawa, K. and I. Hoshino. 1988. Temperature structure and mixed layer in the Kuroshio region over the Izu Ridge. J. Mar. Res., 46, 683-700 https://doi.org/10.1357/002224088785113397
  14. Hansell, D.A. and C.A. Carlson. 2002. Biogeochemistry of Marine Dissolved Organic Matter. Academic Press, San Diego. p. 64-80
  15. Harvey, G.R., D.A. Boran, L.A. Chesal, and J.M. Tokar. 1983. The structure of marine fulvic and humic acids. Mar. Chem., 12, 119-132 https://doi.org/10.1016/0304-4203(83)90075-0
  16. Hayase, K. and N. Shinozuka. 1995. Vertical distribution of fluorescent organic matter along with AOU and nutrients in the equatorial Central Pacific. Mar. Chem., 48, 283-290 https://doi.org/10.1016/0304-4203(94)00051-E
  17. Hill, J.K. and P.A. Wheeler. 2002. Organic carbon and nitrogen in the northern California current system: Comparison of offshore, river plume, and coastally upwelled waters. Prog. Oceanogr., 53, 369-387 https://doi.org/10.1016/S0079-6611(02)00037-X
  18. Holm-Hansen, O. and C.R. Booth. 1966. The measurement of adenosine triphosphate in the ocean and its ecological significance. Limnol. Oceanogr., 11, 510-519 https://doi.org/10.4319/lo.1966.11.4.0510
  19. Kouassi, A.M. and R.G. Zika. 1990. Light induced alteration of the photophysical properties of dissolved organic matter in seawater. Neth. J. Sea Res., 26, 25-32 https://doi.org/10.1016/0077-7579(90)90031-B
  20. Leiner, M.J.P. and O.S. Wolfbeis. 1988. Biochemical application of 3-dimensional fluorescence spectroscopy. p. 134- 138. In: Time-Resolved laser Spectroscopy in Biochemistry. ed. by J.R. Lakowicz. SPIE Proc., 909. Society for Photo-optical Instrumentation Engineers, Bellingham, Washington
  21. Lochmuller, C.H. and S.S. Saavedra. 1986. Conformational changes in a soil fulvic acid measured by timedependent fluorescence depolarization. Anal. Chem., 58, 1978-1981 https://doi.org/10.1021/ac00122a014
  22. Malcolm, R.L. 1990. The uniqueness of humic substance in each of soil, stream and marine environments. Anal. Chim. Acta., 232, 19-30 https://doi.org/10.1016/S0003-2670(00)81222-2
  23. Millero, F.J. 1996. Chemical Oceanography. 2nd ed. CRC Press, p. 220-222, 346-347
  24. Momzikoff, A., S. Dallot, and M.D. Pizay. 1992. Blue and yellow fluorescence of filtered seawater in a frontal zone. Deep Sea Res., 39, 1481-1498 https://doi.org/10.1016/0198-0149(92)90043-S
  25. Mopper, K. and A.S. Schultz. 1993. Fluorescence as a possible tool for studying the nature and water column distribution of DOC compounds. Mar. Chem., 41, 229-238 https://doi.org/10.1016/0304-4203(93)90124-7
  26. Parsons, T.R., Y. Maita, and C.M. Lalli. 1984. A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press, Oxford. 249 p
  27. Park, Y.C., C.H. Yoon, and K.H. Chung. 1994. Amino acid composition and characteristic of dissolved organic compounds in the Yellow Sea. J. Oceanol. Soc. Kor., 29, 171-182
  28. Park, Y.C., S.K. Son, K.H. Chung, and K.H. Kim. 1995. Characteristics of fluorescent organic matter and amino acids composition in the East Sea. J. Oceanol. Soc. Kor., 30, 341-354
  29. Pickard, G.L. and W.J. Emery. 1982. Descriptive Physical Oceanography. Pergamon Press, Oxford. p. 45
  30. Sharp, J.H., Y. Suzuki, and W.L. Munday. 1993. A comparison of dissolved organic carbon in Atlantic continental slope, coastal and estuarine waters by high temperature combustion and wet chemical oxidation. Mar. Chem., 41, 253-259 https://doi.org/10.1016/0304-4203(93)90127-A
  31. Sierra, M.M.D., O.F.X. Donald, M. Lamotte, C. Belin, and M. Ewald. 1994. Fluorescence spectroscopy of coastal and marine waters. Mar. Chem., 47, 127-144 https://doi.org/10.1016/0304-4203(94)90104-X
  32. Suzuki, Y. and E. Tanoue. 1991. Dissolved organic carbon enigma-implication for ocean margin. p. 18-23. In: Ocean Margin Processes in Global Change. Dahlem Workshop. ed. by R.F.C. Mantoura, J.M. Martin and R. Wollast. John Wiley & Sons, New York
  33. Williams, P.M. and E.R.M. Druffel. 1998. Dissolved organic matter in the ocean: comments on a controversy. Oceanography, 1, 14-17
  34. Williams, P.M., J.E. Bauer, K.J. Robertson, and D.M. Wolgast. 1993. Data report on DOC and DON measurements made at SIO, 1988-1991. Mar. Chem., 41, 271-281 https://doi.org/10.1016/0304-4203(93)90130-G