Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Quantitative Change of Aristolochic Acid Contents by Processing Methods on the Plants of Aristolochiaceae

쥐방울과 한약의 수치에 따른 aristolochic acid 함량변화

  • Kim, Min-Suk (Department of Medical Plant Resources, Mokpo National University) ;
  • Lee, Joung-Bok (Saerom Pharmaceutical Co., Oriental Medicinal Processing Institute) ;
  • Park, Si-Hyung (Department of Medical Plant Resources, Mokpo National University) ;
  • Kim, Dong-Wook (Department of Medical Plant Resources, Mokpo National University) ;
  • Min, Oh-Jin (Department of Medical Plant Resources, Mokpo National University) ;
  • Rhyu, Dong-Young (Department of Medical Plant Resources, Mokpo National University)
  • 김민석 (목포대학교 생명과학부 생약자원전공) ;
  • 이정복 (새롬제약 수치법제연구소) ;
  • 박시형 (목포대학교 생명과학부 생약자원전공) ;
  • 김동욱 (목포대학교 생명과학부 생약자원전공) ;
  • 민오진 (목포대학교 생명과학부 생약자원전공) ;
  • 류동영 (목포대학교 생명과학부 생약자원전공)
  • Published : 2007.06.30
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Abstract

Aristolochic acid (AA) included in the plants Aristolochiaceae have been well known to be nephrotoxic and carcinogenic inducer and to cause renal disease such as Chinese Herb Nephropathy (CHN). In this study, we used a high performance liquid chromatopaphy-mass spectrometry (HPLC-MS) under the positive ion detection mode for the quantitative change of aristolochic acid-I and-II (AA-I and AA-II) in Aristolochiaceae (Aristolochia contorta Bunge, Aristolochia debilis Sieb. et Zucc., Aristolochia fangchi Wu), some related plants (Cocculus trilobus De candolle, Inula helenium Linne, Saussurea lappa Clarke), and its prescriptions (防己茯笭湯, 定喘散) with or without processing. Here, the processing methods and prescriptions in oriental medicine were generally used to alleviate toxicity or alter property of herbal medicines. However, the concentrations of AA-I and AA-II were highly determined in processed material extracts rather than unprocessed those, not measured in some related plants. Also, the concentrations of AA-I and AA-II even at the prescriptions mixed the plants of Aristolochiaceae were detected to range from 0.73 to 2.53 ppm. Thus, the present results suggest that the content of AA-I and AA-II contained to plants of Aristolochiaceae was not reduced by the processing methods or prescriptions which can induce the physico-chemical change and pharmacological transformation in traditional herbal medicines.

Keywords

References

  1. Vanherweghem, J. L., Depierreux, M., Tielemans, C., Abramowicz, D., Dratwa, M., Jadoul, M., Richard, C., Vandervelde, D., Verbeelen, D. and Vanhaelen-Fastre, R. (1993) Rapidly progressive interstitial renal fibrosis in young women: association with slimming regimen including Chinese herbs. Lancet 341: 892-893 https://doi.org/10.1016/0140-6736(93)93099-M
  2. Depierreux. M., Van Damme, B., Vanden Houte, K. and Vanherweghem, J. L. (1994) Pathologic aspects of a newly described nephropathy related to the prolonged use of Chinese herbs. Am. J. Kidney. Dis. 24(2): 172-180 https://doi.org/10.1016/S0272-6386(12)80178-8
  3. Lord, G. M., Tagore, R., Cook, T., Gower, P. and Pusey, C. D. (1999) Nephropathy caused by Chinese herbs in the UK. Lancet 354: 481-482 https://doi.org/10.1016/S0140-6736(99)03380-2
  4. Nortier, J. L., Martinez, M. C., Schmeiser, H. H., Arlt, V. M., Bieler, C. A., Petein, M., Depierreux, M. F., De Pauw, L., Abramowicz, D., Vereerstraeten, P. and Vanherweghem, J. L. (2000) Urothelial carcinoma associated with the use of a Chinese herb (Aristolochia fangchi). N. Engl. J. Med. 342(23): 1686-1692 https://doi.org/10.1056/NEJM200006083422301
  5. 손경희, 이규식, 김순선, 김소희, 곽승준, 채수영, 박철훈, 김병호, 최요우, 이종필, 제금련, 강진석, 장동덕, 길광섭, 최광식, 박귀례 (2001) 아리스톨로크산 함유 생약제에 대한 안전성 평가 연구: 생식.발생독성시험연구. 식품의약 품안전청 연구보고서 5(5): 590-602
  6. 황명실, 박미선, 문지영, 이지선, 염영나, 이효민, 신동환, 강진석, 윤은경, 최미나, 육미영, 장동덕, 길광섭, 김승희, 양기화 (2001) 아리스톨로크산 함유 생약제에 대한 안전 성평가연구: 3개월간 반복 투여 독성시험을 통한 신장독성평가. 식품의약안전청 연구보고서 5(5): 568-578
  7. Nortier, J. M. and Vanherweghem, J. L. (2002) Renal interstitial fibrosis and urothelial carcinoma associated with the use of a Chinese herb (Aristolochia fanchi). Toxicology 27(181-182): 577-580
  8. Gillerot. G., Goffin, E., Moulin, P., Arlt, V. M., Phillips, D. H., Cosyns, J. P. and Devuyst, O. (2003) Aristolochic acid nephropathy and the peritoneum: Functional, structural, and molecular studies. Kidney Int. 64(5): 1883-1892 https://doi.org/10.1046/j.1523-1755.2003.00275.x
  9. Schmeiser, H. H., Pool, B. L. and Wiessler, M. (1986) Identification and mutagenicity of metabolites of aristolochic acid formed by rat liver. Carcinogenesis 7(1): 59-63 https://doi.org/10.1093/carcin/7.1.59
  10. Moreno, J. J. (1993) Effect of aristolochic acid on arachidonic acid cascade and in vivo models of inflammation. Immunopharmacology 26(1): 1-9 https://doi.org/10.1016/0162-3109(93)90061-T
  11. Schmeiser, H. H., Bieler, C. A., Wiessler, M., van Ypersele de Strihou, C. and Cosyns, J. P. (1996) Detection of DNA adducts formed by aristolochic acid in renal tissue from patients with Chinese herbs nephropathy. Cancer Res. 56(9): 2025-2028
  12. 한대석, 정보섭, 지형준, 이흠숙 (1989) 한국산 청목향의 성분에 관한 연구(I). Kor. J. Pharmacogn. 20(1): 1-5
  13. Vanhaelen, M., Vanhaelen-Fastre, R., But, P. and Vanherweghem, J. L. (1994) Identification of aristolochic acid in Chinese herbs. Lancet 343: 174
  14. 제금련, 성락선, 이종필, 박상용, 정영자, 조창희, 조소연, 하광원, 이흠숙, 이형규 (2001) 생약 및 생약(한약)제제의 품질평가법 연구: 유통 생약중의 아리스톨로크산의 함량에 관한 연구. 식품의약품안전청 연구보고서 5(5): 167-174
  15. Arlt, V. M., Stiborova, M. and Schmeiser, H. H. (2002) Aristolochic acid as a probable human cancer hazard in herbal remedies: a review. Mutagenesis 17(4): 265-277 https://doi.org/10.1093/mutage/17.4.265
  16. 강숙경, 송경빈 (2003) 국내 유통 한약재 중 Aristolochic Acid 분석. J. Korean Soc. Food Sci. Nutr. 32(7): 1164-1167 https://doi.org/10.3746/jkfn.2003.32.7.1164
  17. Sun, Z., Liu, L., Zheng, X., Fan, C., Wang, Q. and Li, G. (2004) An easy and rapid method to determine aristolochic acids and with high sensitivity. Anal Bioanal Chem. 378(2): 388-390 https://doi.org/10.1007/s00216-003-2209-2
  18. 강수영, 서부일, 최호영 (2003) 한약포제와 임상응용, 15-28. 영림사, 서울
  19. Kitagawa, I., Chen, Z. L., Yoshihara, M., Kobayashi, K., Yoshikawa, M., Ono, N. and Yoshimura, Y. (1984) Chemical studies on crude drug processing. III. Aconiti tuber (2). On the constituents of pao-fuzi, the processed tuber of Aconitum carmichaeli Debx, and biological activities of lipo-alkaloids. Yakugaku Zasshi 104(8): 858-866 https://doi.org/10.1248/yakushi1947.104.8_858
  20. 박신영, 정보섭, 이형규, 이현선, 류종현 (1989) 무독부자 제조의 관한 연구. Kor. J. Pharmacogn. 20(1): 25-31
  21. 이제현, 고정아, 황은영, 홍선표 (2002) 숙지황의 포제에 따른 5-hydroxymethyl-2-furaldehyde 함량. 대한본초학회지 17(2): 145-149
  22. 김남재, 홍남두 (1996) 한약수치에 관한 연구(제 5보) - 수치에 의한 감초의 성분변화 및 생리활성. Kor. J. Pharmacogn. 27(3): 196-206
  23. 서부일, 최호영 (2004) 임상한방본초학, 55-73. 영림사, 서울
  24. 유효통, 노중례, 박윤덕 지음. 신민교, 박경, 맹웅제 옮김 (1989) 향약집성방, 333, 영림사, 서울
  25. 동의치료경험집성방 편찬위원회 (1997) 동의치료경험집성방, 1561
  26. 김기영, 송호준 (2002) 한약포제학, 408-409. 신일상사, 서울
  27. Hashimoto, K., Higuchi, M., Makino, B., Sakakibara, I., Kubo, M., Komatsu, Y., Maruno, M. and Okada, M. (1999) Quantitative analysis of aristolochic acids, toxic compounds, contained in some medicinal plants. J. Ethnopharmacol. 64(2): 185-189 https://doi.org/10.1016/S0378-8741(98)00123-8
  28. Lee, T. Y., Wu, M. L., Deng, J. F. and Hwang, D. F. (2001) High-performance liquid chromatographic determination for aristolochic acid in medicinal plants and slimming products. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 766(1): 169-174 https://doi.org/10.1016/S0378-4347(01)00416-9
  29. Jong, T. T., Lee, M. R., Hsiao, S. S., Hsai, J. L., Wu, T. S., Chiang, S. T. and Cai, S. Q. (2003) Analysis of aristolochic acid in nine sources of Xixin, a traditional Chinese medicine, by liquid chromatography/atmospheric pressure chemical ionization/tandem mass spectrometry. J. Pharm. Biomed. Anal. 33(4): 831-837 https://doi.org/10.1016/S0731-7085(03)00310-8