Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation and Antimicrobial Susceptibility of Campylobacter jejuni from Diarrhea Patients

설사환자에서 Campylobacter jejuni의 분리 및 항생제 감수성 특성

  • Park, Eun-Hee (Department of Microbiology, Busan Metropolitan City Institute of Health & Environment) ;
  • Kim, Joung-A (Department of Microbiology, Busan Metropolitan City Institute of Health & Environment) ;
  • Choi, Seung-Hwa (Department of Microbiology, Busan Metropolitan City Institute of Health & Environment) ;
  • Bin, Jae-Hun (Department of Microbiology, Busan Metropolitan City Institute of Health & Environment) ;
  • Cheigh, Hong-Sik (Department of Microbiology, Busan Metropolitan City Institute of Health & Environment) ;
  • Suk, Dong-Hee (Department of Labolatory medicine, Inje University Dongnae Paik Hospital) ;
  • Lee, Su-Chul (Departmnet of Clinical pathology, ChoonHae Hospital) ;
  • Kim, Young-Hui (Department of Labolatory medicine, Hanseo Hospital)
  • 박은희 (부산광역시보건환경연구원) ;
  • 김정아 (부산광역시보건환경연구원) ;
  • 최성화 (부산광역시보건환경연구원) ;
  • 빈재훈 (부산광역시보건환경연구원) ;
  • 최홍식 (부산광역시보건환경연구원) ;
  • 석동희 (인제대학교 동래백병원 진단검사의학과) ;
  • 이수철 (춘해병원 진단검사의학과) ;
  • 김영희 (한서병원 진단검사의학과)
  • Published : 2007.06.25
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Abstract

ln this study we isolated 27 isolates of Campylobacter jejuni from stool samples of 882 diarrheal patients. The seasonal distribution of patients was highest at July (11.7%). All the isolates of C. jejuni hydrolyzing sodium hippurate were serotyped on basis of heat-stable antigens, and identified with the use of passive hemagglutination assay. A total of 59.3% among 27 C. jejuni isolates were identified into 6 different serotypes, which serotype HS2, HSl/44, and HS2l were dominant. Antibiotics resistant rates of C. jejuni isolates were shown to be 100%, 63.0%, 51.9%, 37.0%, 33.3%, 25.9% and 7.4% to cephalothin, trimethoprim- sulfamethoxazole, tetracycline, ciprofloxacin, ampicillin, gentamycin and clindamycin, respectively. All isolates were sensitive to the erythromycin and imipenem.

2005년 1월부터 2006년 10월까지 설사환자의 분변 882건에서 27주의 C. jejuni가 분리되어 3.1%의 분리율을 보였으며, 계절별로는 7월에 가장 높은 9주(11.7%)가 분리되었으며, 분리균주 모두는 catalase 양성, hippuric acid를 가수분해하였다. heat-stable antigen을 이용한 PHA법으로 확인한 혈청형은 HS2형이 18.5%로 가장 많이 분리되었으며 Hs1/44형과 HS2l형 이 각각 11.1%, HS4 complex와 HSl9형 이 각각 7.4%,그리고 HSl8형이 3.7%였으며, 40.7%인 11주는 본 실험방법으로 혈청형을 확인할 수 없었다. 항생제에 대한 내성정도는 cephalothin에는 100%의 내성률을 erythromycin과 imipenem에는 0%의 내성률을 나타내어 분리균주 모두 감수성이었다. 항생제별로는 trimethoprim-sulfamethoxazole 63.0%, tetracycline 51.9%, ciprofloxacin 37.0%, ampicillin과 nalidixic acid 33.3%, gentamycin 25.9%, clindamycin 7.4%, chlorarmphenicol 3.7%의 순으로 내성률을 보였다.

Keywords

References

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