Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Proteome Analysis of various types of Panax ginseng using 2-Dimensional Electrophoresis

인삼, 산양삼 및 산삼의 부위별 Proteome분석

  • We, Jong-Sung (Dept. of Acupuncture & Moxibustion, Oriental Medical College. Sangjl University) ;
  • Park, Hee-Soo (Dept. of Acupuncture & Moxibustion, Oriental Medical College. Sangjl University) ;
  • Kwon, Ki-Rok (Dept. of Acupuncture & Moxibustion, Oriental Medical College. Sangjl University)
  • 위종성 (상지대학교 한의과대학 침구학교실) ;
  • 박희수 (상지대학교 한의과대학 침구학교실) ;
  • 권기록 (상지대학교 한의과대학 침구학교실)
  • Published : 2007.06.30
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Abstract

Objectives : The purpose of this study was to obtain an objective differentiating method for various types of Panax ginseng: ginseng, cultivated wild ginseng, and natural wild ginseng which are distinctive according to their growing environment. Methods : The roots, stem, and leaves of several types of ginseng were collected and comparative analysis of proteome was conducted on each part using 2-DE and the results examined. Results : 1. Proteome images of the respective parts within the samples showed spot-matching in most cases, suggesting that they are genetically identical panax ginseng. 2. Similar distribution patters were seen within the different parts of the Panax ginseng: ginseng, Chinese cultivated wild ginseng, and the 5 and 10 years old Korean cultivated wild ginseng. 3. For a quantitative evaluation of spots showing differences among the samples, 102 spots from the roots, 109 spots from the stems, and 132 spots form the leaves which showed a difference were selected and centrifugal identification was conducted. 4. Peculiar proteins from each respective part of the Panax ginseng were identified and the top 20 spots with significant differences were selected and analyzed in order to provide a differentiation rate among the samples. The accuracy rate ranged between 23.0-38.8%. 5. Differentiation rate of the top 10 spots with significant differences showed a 50-85% accuracy rate, and the differentiation rate was especially high for the stem of Chinese cultivated wild ginseng and Korean cultivated wild ginseng.

Keywords

References

  1. 우선희, 김홍식, 송범헌, 이철원, 박영목, 정승근, 조용구. 프로테옴 해석에 의한 벼 게놈 기능해석과 응용. 한국식물생명공학회지 2003; 30(3): 281-283 https://doi.org/10.5010/JPB.2003.30.3.281
  2. Park, OK. Proteomic Studies in Plants. J. of Biochemistry and Molecular Biology 2004; 37(1): 133-138 https://doi.org/10.5483/BMBRep.2004.37.1.133
  3. Zolla, L., Timpero, AM, Walcher, W. and Huber, CG. Proteomics of light-harvesting proteins in different plant species. Analysis and comparison by liquid chromatography-electrospray ionization mass spectrometry. Photosystem II. Plant Physiol. 2003; 131: 198-214 https://doi.org/10.1104/pp.012823
  4. Millar. A. H., Sweetlove, L. I., Giege, P. and Leaver, C. J. Analysis of the Arabidopsis mitochondrial proteome. Plant Physiol. 2001; 127: 1711-1727 https://doi.org/10.1104/pp.010387
  5. Koller, A., Washburn, M. P., Lange, B. M., Andon, N. L., Deciu, C., Haynes, P. A., Hays, L., Schieltz, D., Ulaszea, R., Wei, J., Wolters, D. and Yates, J. R. 3rd. Proteomic survey of metabolic pathways in rice. Proc. Natl. Acad. Sci. USA. 2002; 99: 11969-11974 https://doi.org/10.1073/pnas.172183199
  6. Zivy, M. and de Vienne, D. Proteomics: a link between ginomics. genetics and physiology. Plant Mol. Biol. 2000; 44: 575-580 https://doi.org/10.1023/A:1026525406953
  7. 권기록, 서정철. 산삼과 장뇌삼 중 고려삼과 서양삼의 Pyrosequencing법에 의한 감별. 대한본초학회지. 2004; 19(4): 45-50
  8. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  9. Anderson, N.L., et al., A two-dimensional gel database of rat liver proteins useful in gene regulation and drug effects studies, Electrophoresis, 1991: 12; 907-930 https://doi.org/10.1002/elps.1150121110
  10. Shevchenko A, Wilm M, Vorm O, Mann M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem. 1996; 1: 850-858 https://doi.org/10.1021/ac950914h
  11. Rabilloud, T. Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains. Proteomics. 2002; 2: 3-10 https://doi.org/10.1002/1615-9861(200201)2:1<3::AID-PROT3>3.0.CO;2-R
  12. Dongxia Wang, Suzanne R. Kalb and Robert J. Cotter. Improved procedures for N-terminal sulfonation of peptides for matrix-assisted laser desorption/ionization post-source decay peptide sequencing Rapid Commun. Mass Spectrom. 2004; 18: 96-102 https://doi.org/10.1002/rcm.1289
  13. 전국한의과대학 본초학교수공편저, 본초학, 서울, 영림사, 1994; 531
  14. 單書健, 神農本草經校證, 吉林省, 吉林科學技術出版社, 1988; 150
  15. 吳普, 吳普本草, 北京, 人民衛生出版社, 1987; 14
  16. 李時珍, 本草綱目, 北京, 人民衛生出版社, 1982; 699-701
  17. 李梴, 醫學入門, 서울, 대성출판사, 1997; 400
  18. 唐愼微, 重修政和經史證類備用本草, 台北, 南天書局有限公司, 1976; 145
  19. 王好古, 湯液本草, 本草名著集成 北京, 華夏出版社, 1998; 30-31
  20. 汪昻, 本草備要, 本草名著集成 北京, 華夏出版社. 1998; 242-243
  21. 徐彦純, 本草發揮, 歷代本草精華叢書 二, 上海, 上海中醫藥大學出版社. 1994; 510
  22. Byung Sam Yoo, Moon Sik Chang, and Sang Yo Byun. Characterization of cell culture and ginsenoside production by cultured ginseng and wild mountain ginseng. Korean J. Biotechnol. Bioeng. 2003; 18(2): 133-139
  23. Ko, S. R., Choi, K. J., Kim, S. C. and Han, K. H. Content and composition of saponin compounds of Panax species. Korean J. Ginseng Sci. 1995; 19: 254-295

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