Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Phosphate Solubilizing Activity of Pseudomonas sp. CL-1 and Kluyvera sp. CL-2

Pseudomonas sp. CL-1 및 Kluyvera sp. CL-2 균주의 인산가용화 특성

  • Kwon, Jang-Sik (National Institute of Agricultural Science and Technology) ;
  • Suh, Jang-Sun (National Institute of Agricultural Science and Technology) ;
  • Weon, Hang-Yeon (National Institute of Agricultural Science and Technology) ;
  • Kim, Wan-Gyu (National Institute of Agricultural Science and Technology) ;
  • Noh, Hyung-Jun (National Institute of Agricultural Science and Technology)
  • 권장식 (농촌진흥청 농업과학기술원) ;
  • 서장선 (농촌진흥청 농업과학기술원) ;
  • 원항연 (농촌진흥청 농업과학기술원) ;
  • 김완규 (농촌진흥청 농업과학기술원) ;
  • 노형준 (농촌진흥청 농업과학기술원)
  • Received : 2007.09.19
  • Accepted : 2007.10.12
  • Published : 2007.12.30
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Abstract

From the rhizoplane and rhizosphere of pepper, tomato, lettuce, pasture, and grass, unsoluble inorganic phosphate solubilizing bacterial strains were isolated using plate base assay on Pikovskaya's medium. Two strains, CL-1 and CL-2, which produced largest halo on plates (indicative of phosphate solubilization)were selected for further studies. Based on these biochemical and 16S rRNA analysis strains CL-1, CL-2 were found to be as species of Pseudomonas sp. and Kluyvera sp., respectively. In broth assay Pseudomonas sp. CL-1 and Kluyvera sp. CL-2 solubilized insoluble phosphate by 193.4 mg and $493.6P\;mg\;L^{-1}$, respectively after $3^{rd}$ day inoculation. These effecient phosphate solubilizing bacteria have a potential to be developed as microbial based fertilizer in future.

토양에 고정되어 축적된 난용성 인산염을 가용화하는 유용세균을 선발하여 생물비료로 이용하고자 고추, 토마토, 상추, 오이, 목초, 잔디의 근권토양 및 뿌리표면에서 인산가용화능이 있는 세균을 분리하였다. 선발된 인산가용화균은 16S rRNA 염기서열과 생화학적특성 등에 의해 동정되었으며, 난용성인산 가용화기능이 우수한 세균 Pseudomonas sp. CL-1 및 Kluyvera sp. CL-2균주를 선발하였다. Pseudomonas sp. CL-1균주는 esculin과 gelatin, casein을 가수분해하였고, 그리고 glucose, arabinose, mannose, mannitol, N-acetyl-glucosamine, gluconate, caprate, adipate, malate, citrate 등을 이용하였다. Kluyvera sp. CL-2 균주는 esculin과 CM-cellulose를 가수분해 하였고 acetoin을 생성하였다. 그리고 glucose, arabinose, mannose, mannitol, N-acetyl-glucosamine, maltose, gluconate, malate, citrate 등을 이용하였다. Pikovskaya's medium에서 선발균주의 난용성인산 $Ca_3(PO_4)_2$의 인 가용화량을 정량한 결과 Pseudomonas sp. CL-1과 Kluyvera sp. CL-2 균주는 접종후 1일, 3일에 각각 148.0, $193.4(P\;mg\;L^{-1})$와 482.8 mg, 493.6 mg의 인 가용화량을 나타내었다

Keywords

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