Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Increased Carotenoid Production in Xanthophyllomyces dendrorhous G276 Using Plant Extracts

  • Kim, Soo-Ki (Department of Animal Sciences and Environment Konkuk University) ;
  • Lee, Jun-Hyeong (Department of Animal Sciences and Environment Konkuk University) ;
  • Lee, Chi-Ho (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Yoon, Yoh-Chang (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
  • Published : 2007.04.30
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Abstract

The red yeast Xanthophyllomyces dendrorhous (previously named Phaffia rhodozyma) produces astaxanthin pigment among many carotenoids. The mutant X. dendrorhous G276 was isolated by chemical mutagenesis. The mutant produced about 2.0 mg of carotenoid per g of yeast cell dry weight and 8.0 mg/L of carotenoid after 5 days batch culture with YM media; in comparison, the parent strain produced 0.66 mg/g of yeast cell dry weight and a carotenoid concentration of 4.5 mg/L. We characterized the utilization of carbon sources by the mutant strain and screened various edible plant extracts to enhance the carotenoid production. The addition of Perilla frutescens (final concentration, 5%) or Allium fistulosum extracts (final concentration, 1%) enhanced the pigment production to about 32 mg/L. In a batch fermentor, addition of Perilla frutescens extract reduced the cultivation time by two days compared to control (no extract), which usually required five-day incubation to fully produce astaxanthin. The results suggest that plant extracts such as Perilla frutescens can effectively enhance astaxanthin production.

Keywords

References

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