김치에서 분리한 Lactococcus lactis W-44에 의한 감귤발효물의 양식 넙치 성장에 미치는 영향

Effect of Citrus Fermented by Lactococcus lactis W-44 Isolated from Kimchi on Growth of Cultured Flounder, Paralichthys olivaceus

  • 김민수 (한국생명공학연구원) ;
  • 문상욱 ((주)Fermentech) ;
  • 이영돈 (제주대학교 해양과환경연구소) ;
  • 김세재 (제주대학교 생명과학과) ;
  • 김영진 (부산대학교 의과대학 미생물학 및 면역학 교실) ;
  • 이준원 (전남대학교 의과대학 유전자제어 의과학연구센터) ;
  • 이정희 ((주)일해) ;
  • 이정숙 (한국생명공학연구원) ;
  • 김보연 (한국생명공학연구원) ;
  • 안종석 (한국생명공학연구원) ;
  • 안순철 (부산대학교 의과대학 미생물학 및 면역학 교실)
  • Kim, Min-Soo (Korea Research Institute of Bioscience and Biotechnology) ;
  • Moon, Sang-Wook (Fermentech Co.) ;
  • Lee, Young-Don (Marine and Environment Research Institute, Cheju National University) ;
  • Kim, Se-Jae (Deparaent of Biological Science, Cheju National University) ;
  • Kim, Yeong-Jin (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Lee, Jun-Won (Medical Research Center For Gene Regulation, Medical School, Chonnam National University) ;
  • Lee, Jeong-Hee (Il Hae Co.) ;
  • Lee, Jung-Sook (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Bo-Yeon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Jong-Seog (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Soon-Cheol (Department of Microbiology and Immunology, College of Medicine, Pusan National University)
  • 발행 : 2007.06.30

초록

젖산균을 접종하여 감귤 분쇄액을 발효시켜 얻은 감귤발효물의 어류양식 사료 첨가제로의 가능성을 넙치(Paralichthys olivaceus) 양식에서 검토하였다. 김치에서 항균활성이 우수한 젖산균 W-44를 분리한 후 16S rDNA 염기서열 분식을 통하여 Lactococcus lactis W-44로 동정하였다. L. lactis W-44를 이용하여 감귤 분쇄액 발효를 수행한 결과, 생리활성이 우수한 무배당체 flavonoids인 naringenin, hesperitin의 함량이 각각 약 10과 6배 증가함을 확인하였다. L. lactis W-44를 이용하여 발효된 감귤발효액을 양식넙치의 사료첨가제로 투여한 결과, 감귤발효액을 투여하지 않은 대조구의 평균 전장 및 체중 증가율과 현격한 차이가 있었다. 또한 0.2% (v/v)의 감귤발효액을 투여한 실험군에서 넙치의 성장이 가장 우수한 것으로 나타났다. 이러한 결과로부터 젖산균의 감귤발효산물을 넙치 양식에서 기능성 사료첨가제로 사용할 수 있는 가능성을 확인하였다.

We evaluated the use of citrus fruit fermented by lactic acid bacteria, as a feed supplement for flounder (Paralichthys olivaceus) cultivation. For the fermentation, a lactic acid bacterial strain W-44 showing antibacterial activity was isolated from kimchi. From the phylogenetic analysis based on, 16S rDNA sequence, the strain W-44 was identified as Lactococcus lactis. After the fermentation of citrus fruit with L. lactis W-44, the contents of naringenin and hesperetin, bioactive flavonoid aglycones, were increased about ten-fold and six-fold, respectively. The effects of fermented citrus fruit-based feed additives (CFBFA) were tested on the growth of flounder, Paralichthys olivaceus. There were significant differences in average total length and body weight between the experimental and control group. The growth rate of the experimental group fed with the 0.2% CFBFA-supplemented diet was increased 4.5% and 20.9% more than the control group in total length and body weight, respectively. These results suggest that the fermented citrus fruit could be used as a functional feed additive for flounder cultivation.

키워드

참고문헌

  1. Cha, J.Y., S.Y. Kim, S.J. Jeong, and Y.S. Cho. 1999. Effects of hesperitin and naringenin on lipid concentration in orotic acid treated mouse. Kor. J. Life Science 9, 389-394
  2. Chyung, M.K. 1991. The fish of Korean, p. 727. Iljisa Pub. Co., Seoul
  3. Eun, J.B., Y.M. Jung, and G.J. Woo. 1996. Identification and determination of dietary fibers and flavonoids in pulp and peel of Korean tangerine (Citrus aurantium var.). Kor. J. Food Sci. Technol. 28, 371-377
  4. Frydoonfar, H.R., D.R. McGrath, and A.D. Spigelman. 2003. The variable effect on proliferation of a colon cancer cell line by the citrus fruit flavonoid naringenin. Colorectal Dis. 5, 149-152 https://doi.org/10.1046/j.1463-1318.2003.00444.x
  5. Harris, L.J., M.A. Daeschel, M.E. Stiles, and T.R. Klaenhahammer. 1989. Antimicrobial activity of lactic aicd bacteria against Listeria monocytogenes. J. Food Prot. 52, 384-387 https://doi.org/10.4315/0362-028X-52.6.384
  6. Hwang, M.H., S.I. Park, and Y.C. Kim. 1999. Effect of dietary herb medicinal stuff on the non-specific immune response of nile tilapia, Oreochromis niloticus. J. Fish. Pathol. 12, 7-14
  7. Izumi, T., M.K. Piskula, S. Osawa, A. Obata, K. Tobe, M. Saito, S. Kataoka, Y. Kubota, and M. Kikuchi. 2000. Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans. J. Nutrition 130, 1695-1699 https://doi.org/10.1093/jn/130.7.1695
  8. Jung, S.H., J.S. Lee, H.K. Han, C.Y. Jun, and H.Y. Lee. 2002. Effects of medicinal herb extract on non-specific immune response, hematology and disease resistance on olive flounder, Paralichthys olivaceus. by oral administration. J. Fish Pathol. 15, 25-35
  9. Kawaguchi, K., T. Mizuno, K. Aida, and K. Uchino. 1999. Hesperidin as an inhibitor of lipase from porcine pancrease and Pseudomonas. Biosci. Biotechnol. Biochem. 61, 102-104 https://doi.org/10.1271/bbb.61.102
  10. Kawaii, S., Y. Tomono, E. Katase, K. Ogawa, and M. Yano. 1999. Quantification of flavonoid constituents in citrus fruits. J. Agric. Food Chem. 47, 3565-3571 https://doi.org/10.1021/jf990153+
  11. Kim, D.S., C.H. Noh, S.W. Jung, and J.Y. Jo. 1998. Effects of obosan supplemented diet on growth, feed conversion ratio and body composition of nile tilapia, Oreochromis niloticus. J. Aquacult. 11, 83-90
  12. Kim, D.S., J.H. Kim, C.H. Jeong, S.Y. Lee, S.M. Lee, and Y.B. Moon. 1998. Utilization of Obosan (dietary herb) I. Effects on survival, growth, feed conversion ratio and condition factor on olive flounder, Paralichthys olivaceus. J. Aquacult. 11, 213-221
  13. Kim, K.H., Y.J. Hwang, and S.K. Chun. 1999. Resistance to Vibrio alginolyticus in juvenile rockfish (Sebastes schlegeli) fed diets containing different doses of aloe. Aquaculture 180, 13-21 https://doi.org/10.1016/S0044-8486(99)00143-X
  14. Kim, M.J., S.C. Chung, and C.B. Song. 2004. Effect of Salinity on Growth and survival of olive flounder, Paralichthys olivaceus. Korean J. Ichthol. 16, 100-106
  15. Kwon, M.G., Y.C. Sohn, and S.I. Park. 1999. The dietary supplementing effects of Kugija, Lycium chinense, on immune response of nile tilapia, Oreochromis niloticus. J. Fish. Pathol. 12, 73-81
  16. Lee, K.H., Y.S. Lee, J.H. Kim, and D.S. Kim. 1998. Utilization of obosan (dietary herb) II. Muscle quality of olive flounder, Paralichthys olivaceus fed with diet containing obosan. J. Aquacult. 11, 319-325
  17. Miyao, S. and T. Ogawa. 1998. Selective media for enumerating lactic acid bacteria groups from fermented pickles, Shouhin Kogyo. Gakkaishi 35, 610-617
  18. Monforte, M.T., A. Trovato, S. Kirjavaninen, A.M. Forestieri, and E.M. Galati. 1995. Biological effect of hesperidin, a citrus flavonoid. Farmco. 50, 595-599
  19. Moon, S.W., S.H. Kang, Y.J. Jin, J.G. Park, Y.D. Lee, Y.K. Lee, D.B. Park, and S.J. Kim. 2004. Fermentation of Citrus unshiu Marc. and Functional Characteristics of the Fermented Products. Kor. J. Food Sci. Technol. 36, 669-676
  20. Mouly, P.P., C.G. Arzouyan, E.M. Gaydou, and J.M. Estienne. 1994. Differentiation of citrus juices by factorial discriminant analysis using liquid chromatography of flavanone glycosides. J. Agric. Food Chem. 42, 70-79 https://doi.org/10.1021/jf00037a011
  21. Oliveira, E.J. and D.G. Watson. 2000. Liquid chromatographymass spectrometry in the study of the metabolism of drugs and other xenobiotics. Biomed. Chromatogr. 14, 351-372 https://doi.org/10.1002/1099-0801(200010)14:6<351::AID-BMC28>3.0.CO;2-2
  22. Park, S.W., J.K. Kwak, J.G. Koo, and M.J. Cho. 2001. Effects of glucan from Schizophillum commune on non-specific immune parameters in common carp (Cyprinus caprio) and flounder (Paralichthys olivaceus) by oral administration. J. Kor. Fish Soc. 34, 412-418
  23. Rousff, R.I., S.F. Martin, and C.O. Youtsey. 1987. Qquantitative survey of narirutin, naringin, hesperidin, and neohesperidin in citrus. J. Agric. Food Chem. 35, 1027-1030 https://doi.org/10.1021/jf00078a040
  24. Sohn, J.S. and M.K. Kim. 1998. Effects of hesperidin and naringin on oxidative capacity in the rat. Korean Nutr. Soc. 3, 687-696
  25. Song, Y.B., S.W. Moon, and Y.D. Lee. 2002. Effect of EM-fermented orange in commercial diet on growth of juvenile flounder. J. Aquacult. 15, 103-110
  26. Tamimoto, S.Y., K. Koike, and S. Takahashi. 1993. Improvement of boiled meat texture of cultured eel by feeding of tochu leaf powder. Biosci. Biotechnol. Biochem. 7, 325-327
  27. Woese, C.R. 1987. Bacterial evolution. Microbiol. Rev. 51, 221-271