Textile Coloration and Finishing (한국염색가공학회지)

The Korean Society of Dyers and Finishers (한국염색가공학회)
권호 표지

Optical detection of protein patterns using 1,3-bisdicyanovinylindane

1,3-bisdicyanovinylindane을 이용한 단백질 패터닝의 광학적 감지

  • Park, Young-Min (BK21 FTIT, Dept. of Organic Materials and Textile System Engineering, Chungnam National University) ;
  • Lee, Ji-Hye (Dept. of Chemical Engineering, Chungnam National University) ;
  • Lee, Chang-Soo (Dept. of Chemical Engineering, Chungnam National University) ;
  • Son, Young-A (BK21 FTIT, Dept. of Organic Materials and Textile System Engineering, Chungnam National University)
  • 박영민 (BK21 FTIT 유기소재.섬유시스템, 충남대학교) ;
  • 이지혜 (화학공학, 바이오응용화학부, 충남대학교) ;
  • 이창수 (화학공학, 바이오응용화학부, 충남대학교) ;
  • 손영아 (BK21 FTIT 유기소재.섬유시스템, 충남대학교)
  • Published : 2007.08.27
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Abstract

In this study, we have obtained the protein patterns using the membrane patterning of soft-lithography technique. The rapid detection of protein including bovine serum albumin (BSA) was resulted from the interaction with 1,3-bisdicyanovinylindane. For the proof of the interaction between BSA and dye, the UV-vis absorption spectra of BSA and dye were observed at 278 nm and 580 nm, respectively. As expected, the absorption spectrum of the interaction between BSA and dye was observed at 584nm. The absorption spectrum of the interaction was red-shifted. In addition, the optical images of the selectively reacted protein patterns showed the distinctive change of patterned color at different pH conditions. Because the dye has negative charges, the charge of BSA at different pH conditions could influence the interaction behavior between dye and BSA. Therefore, in the case of pH 7, the selectively patterned protein substrates obtained deep blue color pattern caused by electrostatic interaction between negative charges of the dye and positive charges of the BSA. However, in the case of pH 10, selectively patterned protein substrates obtained light blue color pattern because the electrostatic interaction was relatively lower than pH 7 due to the change of overall charge distribution of BSA.

Keywords

References

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