Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Analysis of the Stress Effects of Endocrine Disrupting Chemicals (EDCs) on Escherichia coli

  • Kim, Yeon-Seok (College of Life Sciences and Biotechnology, Korea University) ;
  • Min, Ji-Ho (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)) ;
  • Hong, Han-Na (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)) ;
  • Park, Ji-Hyun (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)) ;
  • Park, Kyeong-Seo (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST)) ;
  • Gu, Man-Bock (College of Life Sciences and Biotechnology, Korea University)
  • Published : 2007.08.30
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Abstract

In this study, three of the representative EDCs, $17{\beta}$-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas $17{\beta}$-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli.

Keywords

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