Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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The Identification of HSC70 as a Biomarker for Copper Exposure in Medaka Fish

송사리 모델계에서 구리 노출에 대한 생물지표로서 HSC70의 동정

  • Kim, Woo-Keun (Environmental Toxicology Team, Korea Institute of Toxicology) ;
  • Lee, Sung-Kyu (Environmental Toxicology Team, Korea Institute of Toxicology) ;
  • Kim, Jong-Sang (Department of Animal Science and Biotechnology, Kyungpook National University)
  • 김우근 (한국화학연구원 부설 안전성평가연구소 환경독성시험연구부) ;
  • 이성규 (한국화학연구원 부설 안전성평가연구소 환경독성시험연구부) ;
  • 김정상 (경북대학교 동물공학과)
  • Published : 2007.09.30
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Abstract

구리는 환경에 광범위하게 존재하며, 생물체에게 필수적인 무기질이지만 고농도로 존재할 경우 독성을 발휘한다. 본 연구는 프로티옴 기술을 응용하여 수서태계에 구리와 같은 중금속의 존재 여부를 신속하게 평가하기 위한 생물지표를 발굴하기 위하여 수행되었다. 즉, 송사리(Oryzias latipes)를 이용하여 여러 농도의 구리용액(0.1, 1, 5 mg/L)에 24시간 노출시킨 다음, 머리부분에서 선택적으로 발현이 증가되는 단백질을 동정하고자 시도하였다. 본 시스템에서 유의적으로 발현이 증가하는 것으로 나타난 단백질은 beta-tubulin, heat shock cognate 70 (hsc70)이었으며, 이 결과의 일부를 semi-quantitative RT-PCR를 이용하여 확인하였다. 이와 같이 구리 처리에 특이적으로 발현이 증가된 송사리 단백질들은 노출평가를 위한 생물지표로서 개발을 위하여 더 연구할 가치가 있는 것으로 평가된다.

Keywords

References

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