Characteristics and Antioxidative Activity of Volatile Compounds in Heated Garlic (Allium sativum)

  • Woo, Koan-Sik (Department of Food Science and Technology, Chungbuk National University) ;
  • Yoon, Hyang-Sik (Chungcheongbuk-do Agricultural Research and Extension Service) ;
  • Lee, Youn-Ri (Department of Food Science and Technology, Chungbuk National University) ;
  • Lee, Jun-Soo (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Dae-Joong (Veternary Medicine, Chungbuk National University) ;
  • Hong, Jin-Tae (College of Pharmacy, Chungbuk National University) ;
  • Jeong, Heon-Sang (Department of Food Science and Technology, Chungbuk National University)
  • Published : 2007.10.31


The aroma characteristics and antioxidative activity of volatile compounds in heat-treated garlic (Allium sativum L.) were evaluated. The garlic was heated to various temperatures (100, 110, 120, and $130^{\circ}C$) for different lengths of time (1, 2, and 3 hr). The volatile compounds of heated garlic were extracted by simultaneous steam distillation extraction (SDE). Aroma compound profiles were analyzed by gas chromatography/mass spectrometry (GC/MS) and antioxidative activity was measured by 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation decolorization assay. The major aroma compounds were sulfur compounds such as dimethyl disulfide, 2-propen-1-ol, methyl-2-propenyl disulfide, dimethyl trisulfide, diallyl disulfide, methyl-2-propenyl trisulfide, and di-2-propenyl trisulfide. DPPH radical scavenging activity (EDA, %) and the ascorbic acid equivalent antioxidant activity (AEAC) of volatile compounds in heated garlic increased significantly with the increase of temperature and time (p<0.001). The EDA (%) and AEAC of raw garlic were 26.8%/10 mg garlic and 39.05 mg ascorbic acid equivalent per g sample. After heat treatment, the highest values were 40.50%/10 mg garlic for EDA (%) and 46.43 mg ascorbic acid equivalent per g sample for ABTS.


  1. Nagourney RA. Garlic: Medicinal food or nutritious medicine. J. Med. Food 1: 13-28 (1998)
  2. Block E. The organosulfur chemistry of the genus Allium - Implications for the organic chemistry of sulfur. Angew. Chem. Int. Edit. 31: 1135-1178 (1992)
  3. Rivlin RS. Historical perspective on the use of garlic. J. Nutr. 131: 951-954 (2001)
  4. Lee EJ, Kim KS, Jung HY, Kim DH, Jang HD. Antioxidant activities of garlic (Allum sativum L.) with growing districts. Food Sci. Biotechnol. 14: 123-130 (2005)
  5. Kim YJ, Chang YH, Jeong JH. Changes of cholesterol and selenium levels, and fatty acid composition in broiler meat fed with garlic powder. Food Sci. Biotechnol. 14: 207-211 (2005)
  6. Kimbaris AC, Siatis NG, Daferera DJ, Tarantilis PA, Pappas CS, Polissiou MG. Comparison of distillation and ultrasound-assisted extraction methods for the isolation of sensitive aroma compounds from garlic (Allium sativum). Ultrason. Sonochem. 13: 54-60 (2006)
  7. Rabinkov A, Miron T, Konstantinovski L, Wilchek M, Mirelman D, Weiner L. The mode of action of allicin: Trapping of radicals and interaction with thiol containing proteins. Biophys. Acta 1379: 233- 244 (1998)
  8. Rabinkov A, Miron T, Mirelman D, Wilchek M, Glozman S, Yavin E, Weiner L. S-Allylmercaptoglutathione: The reaction product of allicin with glutathione possesses SH-modifying and antioxidant properties. Biochim. Biophys. Acta 1499: 144-153 (2000)
  9. Deruaz D, Soussan-Marchal F, Joseph I. Analytical strategy by coupling headspace gas chromatography, atomic emission spectrometric detection, and mass spectrometry application to sulfur compounds from garlic. J. Chromatogr. A 677: 345-354 (1994)
  10. Bonaduce I, Colombini MP, Diring S. Identification of garlic in old gildings by gas chromatography–mass spectrometry. J. Chromatogr. A 1107: 226-232 (2006)
  11. Artacho R, Serrano MF, Lopez MD. Determination of organic sulphur compounds in garlic extracts by gas chromatography and mass spectrometry. Food Chem. 53: 91-93 (1995)
  12. Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee J, Jeong HS. Change of Korean ginseng components with high temperature and pressure treatment. Korean J. Food Sci. Technol. 38: 521-525 (2006)
  13. Kwon OC, Woo KS, Kim TM, Kim DJ, Hong JT, Jeong HS. Physicochemical characteristics of garlic (Allium sativum L.) on the high temperature and pressure treatment. Korean J. Food Sci. Technol. 38: 331-336 (2006)
  14. Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS. Change of physicochemical characteristics of Korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J. Food Sci. Technol. 38: 342-347 (2006)
  15. Woo KS, Jang KI, Kim KY, Lee HB, Jeong HS. Antioxidative activity of heat treated licorice (Glycyrrhiza uralensis Fisch) extracts. Korean J. Food Sci. Technol. 38: 355-360 (2006)
  16. Dewanto V, Wu X, Adom KK, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. Agr. Food Chem. 50: 3010-3014 (2002)
  17. Jeong SM, Kim SY, Kim DR, Jo SC, Nam KC, Ahn DU, Lee SC. Effect of heat treatment on the antioxidant activity of extracts from citrus peels. J. Agr. Food Chem. 52: 3389-3393 (2004)
  18. Peleg H, Naim M, Rouseff RL, Zehavi U. Distribution of bound and free polyphenolic acids in oranges (Citrus sinensis) and grapefruit (Citrus paradise). J. Sci. Food Agr. 57: 417-426 (1991)
  19. Choi Y, Lee SM, Chun J, Lee HB, Lee J. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of shiitake (Lentinus edodes) mushroom. Food Chem. 99: 381-387 (2006)
  20. Nickerson GB, Likens ST. Gas chromatographic evidence for the occurrence of hop oil components in beer. J. Chromatogr. A 21: 1-5 (1966)
  21. Tepe B, Sokmen M, Akpulat HA, Sokmen A. Screening of the antioxidant potentials of six salvia species from Turkey. Food Chem. 95: 200-204 (2006)
  22. Lim HK, Yoo ES, Moon JY, Jeon YJ, Cho SK. Antioxidant activity of extracts from dangyuja (Citrus grandis Osbeck) fruits produced in Jeju island. Food Sci. Biotechnol. 15: 312-316 (2006)
  23. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26: 1231-1237 (1999)
  24. Forni LG, Mora-Arellano VO, Packer JE, Willson RL. Nitrogen dioxide and related free radicals: Electron-transfer reactions with organic compounds in solutions containing nitrite or nitrate. J. Chem. Soc. 2: 1-6 (1986)
  25. Kim YD, Seo JS, Kim KJ, Kim KM, Hur CK, Cho IK. Component analysis by different heat treatments of garlic (Allium saivum L.). Korean J. Food Pres. 12:161-165 (2005)
  26. Gorinstein S, Drzewiecki J, Leontowicz H, Leontowicz M, Najman K, Jastrzebski Z, Zachwieja Z, Barton H, Shtabsky B, Katrich E, Trakhtenbergx S. Comparison of the bioactive compounds and antioxidant potentials of fresh and cooked Polish, Ukrainian, and Israeli garlic. J. Agr. Food Chem. 53: 2726-2732 (2005)
  27. Nicoli MC, Anese M, Parpinel MT, Franceschi S, Lerici CR. Loss and/or formation of antioxidants during food processing and storage. Cancer Lett. 114: 71-74 (1997)
  28. Manzocco L, Calligaris S, Mastrocola D, Nicoli MC, Lerici CR. Review of non-enzymatic browning and antioxidant capacity in processed foods. Trends Food Sci. Tech. 11: 340-346 (2001)