Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Pilot Production of Bacterial Cellulose by Gluconacetobacter hansenii TL-2C

Gluconacetobacter hansenii TL-2C에 의한 Bacterial Cellulose의 Pilot 생산

  • Jeong, Ji-Suk (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Kim, Jong-Sun (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Choi, Kyoung-Ho (Dept. of Food Science and Nutrition, Catholic University of Daegu)
  • 정지숙 (대구가톨릭대학교 식품영양학과) ;
  • 김종순 (대구가톨릭대학교 식품영양학과) ;
  • 최경호 (대구가톨릭대학교 식품영양학과)
  • Published : 2007.10.30
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Abstract

This study was designed to ultimately develop a highly efficient mass production technology of bacterial cellulose isolated from the citrus gel fermented by G. hansenii TL-2C. Pilot equipment made with FRP vessel length (665 mm) ${\times}$ width (375 mm) ${\times}$ height (210 mm) was developed for mass production of the citrus gel. To develop the optimal conditions for mass production of citrus gel, comprised of citrus juice (6,000 mL) diluted 100 times, containing 5% seed bacteria, 10% sucrose, and 1% ethanol, citrus juice was fermented at $30^{\circ}C$ for 14 days, and gel productivity in pilot system was examined. BC was isolated and purified from the citrus gel, and their chemical composition and physicochemical properties were investigated.

본 연구에서는 국내산 감귤과즙을 이용하여 고기능성 세균 셀룰로오스를 대량생산하기 위해 감귤과즙으로부터 감귤 내성의 Gluconacetobacter hansenii TL-2C를 선별한 후 pilot 시설을 이용하여 감귤겔을 대량 생산할 수 있는 방법을 개발하였다. 유기산의 약 80%가 citric acid로 내성균의 분리가 부득이한 것으로 판정되어, 겔 발효균의 모체인 tea fungus에서 G. hanenii TF-2를 분리, 여기에 UV를 조사하여 TL-2를 분리한 후, 감귤배지에서 반복배양하여 citrate내성균인 G. hansenii TL-2C를 분리하였다. 감귤농축액 100배 희석액에 initial sucrose 함량 10%(w/v), ethanol 1%(v/v) 을 첨가한 배양액에 종균을 5%(w/v)접종하였다. 대형 4각의 FRP 용기에 뚜껑을 덮고 과즙의 높이를 26 mm로 충진하였으며, $30^{\circ}C$ 배양실에서 14일간 정치배양한 후 세로 360 mm,가로 650 mm, 두께 25 mm 이상의 대형 겔을 지속적으로 생산할 수 있었다.

Keywords

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