Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Anti-Obesity Activity, Anti-Cancer Activity and Single Oral Dose Toxicity of Inonotus xeranticus Extracts

기와층버섯 추출물의 항비만활성, 항암활성 및 단회경구독성시험

  • Kang, Eun-Hee (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, In-Kyoung (Korea Research Institute of Bioscience and Biotechnology) ;
  • Hwang, Mi-Hyun (College of Veterinary Medicine, Kyungpook National University) ;
  • Choi, Jae-Young (College of Veterinary Medicine, Kyungpook National University) ;
  • Chang, Zhi-Qiang (College of Veterinary Medicine, Kyungpook National University) ;
  • Rhee, Man-Hee (College of Veterinary Medicine, Kyungpook National University) ;
  • Yun, Bong-Sik (Korea Research Institute of Bioscience and Biotechnology) ;
  • Jiang, Cheng-Zhe (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Peking Union Medica College) ;
  • Kim, Kil-Soo (College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Seung-Chun (College of Veterinary Medicine, Kyungpook National University)
  • Published : 2007.09.30
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Abstract

In this study, we investigated the in vitro anti-obesity, anti-cancer activity and single oral dose toxicity of Inonotus xeranticus extracted by methanol (INXM) or ethyl acetate (INXE). In order to investigate anti-obestity effect of Inonotus xeranticus extracts, the 3T3-L1 cells were treated with these extracts at various concentrations(1, 10, 100 and $300{\mu}g/ml$). It was observed that 3T3-L1 cells treated with $100{\mu}g/ml$ of Inonotus obliquus ethyl acetate extract (INOE), INXM and INXE, in the absence of differentiation cocktail (0.5mM isobutylmethylxanthine (IBMX) $1{\mu}M$ dexamethasone, $1{\mu}M$ insulin), differentiated at a rate of 78.5, 80.9, and 76.4% respectively. Differentiation rates of 86.6% and 83.4% were observed in 3T3-L1 cells which were treated with differentiation cocktail at $100{\mu}g/ml$ of INXM and INXE, respectively. The anti-cancer effect of Inonotus xeranticus extracts was investigated using a method of sulforhodamine B in sarcoma 180 cell line. The cells were treated with these extracts (1, 10, 100 and $300{\mu}g/ml$) for 48 hours. The growth of cells which were treated with $300{\mu}g/ml$ of INXM was inhibited by 80.1%. The growth of sarcoma 180 cells which were treated with 100 and $300{\mu}g/ml$ of INXE was inhibited by 74.7% and 64.5%, respectively. In single oral dose toxicity study, no differences were observed between control and treated groups in clinical signs, body weight gains, and feed and water consumptions. The results indicated that Inonotus xeranticus extracts did not show any toxic effects at 2,000mg/kg in mice, and the $LD_{50}$ of these extracts was found to be higher than 2,000 mg/kg in this experiment. From the above results, Inonotus xeranticus methanol and ethyl acetate extracts might have useful clinical applications in the management of cancer and obesity and may also be useful as a medicinal food.

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References

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