Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Analysis of Immunomodulating Activities in Methanol Extracts from Several Kinds of Grapes

포도 품종별 메탄올 추출물로부터 면역활성 분석

  • Heo, Jin-Chul (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University) ;
  • Woo, Sang-Uk (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University) ;
  • Kweon, Mi-Ae (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University) ;
  • Kim, Bo-Bae (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University) ;
  • Lee, Sook-Hee (GyeongSangBukDo Agricultural Research & Extension Services) ;
  • Lee, Jin-Man (Department of Food & Biotechnology, Hoseo University) ;
  • Choi, Jong-Uck (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University) ;
  • Chung, Shin-Kyo (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University) ;
  • Lee, Sang-Han (Food & Bio-Industry Institute, and Department of Food Science & Technology, Kyungpook National University)
  • 허진철 (경북대학교 식품생물산업연구소 및 식품공학과) ;
  • 우상욱 (경북대학교 식품생물산업연구소 및 식품공학과) ;
  • 권미애 (경북대학교 식품생물산업연구소 및 식품공학과) ;
  • 김보배 (경북대학교 식품생물산업연구소 및 식품공학과) ;
  • 이숙희 (경북농업기술원) ;
  • 이진만 (호서대학교) ;
  • 최종욱 (경북대학교 식품생물산업연구소 및 식품공학과) ;
  • 정신교 (경북대학교 식품생물산업연구소 및 식품공학과) ;
  • 이상한 (경북대학교 식품생물산업연구소 및 식품공학과)
  • Published : 2007.08.28
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Abstract

Fruits and vegetable extracts are well-known as healthy foods. Such foods have been used as herbal medicines or traditional therapies for centuries. To assess biological activities in grapes, we examined the immunomodulating activities of water extracts from four kinds of grapes (cultivars Kyoho, Delaware, Campbell, and Niagara). We explored possible antioxidant and anticancer activities using antioxidant assays such as the 1,1-diphenyl-2-picrylhydrazyl (DPPH) reduction assay, the ferric iron reducing ability of plasma (FRAP) assay, a cell proliferation assay, an NO inhibition assay, a wound healing assay, and an IL-4/IL-13 elicitation assay. Methanol extracts of grapes were tested. The results showed that each grape extract had potent antioxidant activities. The grape extracts increased cell proliferation and NO production activities in tumor cell lines. IL-4 and IL-13 cytokine levels were decreased in mouse primary spleen cells by treatment with any extract. These results suggest that grape extracts can be used as biomaterials with immunomodulating activities.

하우스 포도 중 대표적인 것으로는 거봉, 델라웨어, 캠벨, 청포도를 열거할 수 있는데, 이에 대한 생물학적 활성 효과 중 항산화와 항암 활성에 대한 보고는 많으나 면역활성에 대한 활성의 연구는 미흡한 실정이다. 이에 우리는 4종의 포도의 메탄올 추출물로 항산화, 항암, 그리고 면역활성을 분석하였다. 전자공유능을 측정하기 위한 DPPH를 이용한 실험 결과 포도 4종에서 모두 항산화 활성을 나타내었으며, 환원력을 알아보기 위한 FRAP 실험 또한 활성을 나타내었다. 산화스트레스에 의한 세포사멸 억제 효과는 약간 있는 것으로 나타났다. 반면에 포도 추출물에 의한 cell proliferation 활성은 증가하는 것으로 나타났다. Nitric oxide (NO) 생성 억제 실험에서는 LPS에 의해 유도된 NO의 활성을 감소시키지는 않으나 포도 추출물 자제가 Raw 264.7 세포에서 NO의 활성을 유도하는 것으로 나타났다. Wound healing assay를 이용하여 항암효과를 알아본 결과 포도추출물 4종에서 세포의 운동성을 억제하는 효과를 가지는 것으로 나타났다. Mouse primary spleen cell 에서의 cytokine IL-4, IL-13의 활성을 알아본 결과 Con A로 유도된 IL-4와 IL-13의 발현 양을 현저히 줄이는 것으로 나타났다. 이로 미루어 보아, 포도 4종에 대한 생물학적 활성 결과 항산화, 항암활성은 물론 항천식 활성이 있는 것으로 확인되어 면역조절 활성이 포도의 기능성에 중요한 역할을 할 것으로 판단된다.

Keywords

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