Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

The Abanones, Haliotis discus hannai, Exhibit Potential Anticoagulant Activity in Normal Sprague Dawley Rats

정상 Sprague Dawley 쥐에 대한 전복의 항응고능에 관한 효과

  • Kim, Hag-Lyeol (Solar salt Biotechnology Research Center, Mokpo National University) ;
  • Kim, Seon-Jae (Division of Food Technology & Nutrition, Chonnam National University) ;
  • Kim, Du-Woon (Division of Food Technology & Nutrition, Chonnam National University) ;
  • Ma, Seung-Jin (Department of Food Science & Technology and Food Industrial Technology Research Center, Mokpo National University) ;
  • Gao, Tiancheng (Department of Food Science & Technology and Food Industrial Technology Research Center, Mokpo National University) ;
  • Li, Hua (Department of Food Science & Technology and Food Industrial Technology Research Center, Mokpo National University) ;
  • Lee, Tae-Hoon (Department of Food Science & Technology and Food Industrial Technology Research Center, Mokpo National University) ;
  • Kim, In-Cheol (Department of Food Science & Technology and Food Industrial Technology Research Center, Mokpo National University) ;
  • Ham, Kyung-Sik (Solar salt Biotechnology Research Center, Mokpo National University) ;
  • Kang, Seong-Gook (Solar salt Biotechnology Research Center, Mokpo National University)
  • 김학렬 (목포대학교 천일염생명과학연구소) ;
  • 김선재 (전남대학교 식품공학.영양학부) ;
  • 김두운 (전남대학교 식품공학.영양학부) ;
  • 마승진 (목포대학교 식품공학과 및 식품산업기술연구센터) ;
  • ;
  • ;
  • 이태훈 (목포대학교 식품공학과 및 식품산업기술연구센터) ;
  • 김인철 (목포대학교 식품공학과 및 식품산업기술연구센터) ;
  • 함경식 (목포대학교 천일염생명과학연구소) ;
  • 강성국 (목포대학교 천일염생명과학연구소)
  • Published : 2007.08.28
Download PDF
⟨ Previous Next ⟩

Abstract

The primary objective of this study was to determine the effects of abalone in reducing blood pressure and increasing anti-coagulant capacity. The serum angiotensin-converting-enzyme (ACE) activities of rats on an abalone-supplemented diet did not significantly differ from the ACE levels of rats on a normal diet, at any time (before the experiment, or 1 week, 2 weeks, 3 weeks, and 4 weeks, after commencement of the abalone diet) during the experiment. This result showed that abalone-supplemented diets had no effect on the activity of ACE, which controls blood pressure. To determine if an abalone-containing diet might increase anti-coagulant capacity, both prothrombin (PT) and activated partial thromboplastin time (APTT) levels were measured. The PT levels of control rats remained constant throughout the experiment. In rats fed the abalone-containing diet, PT levels increased with time, and the increase became statistically significant after 2 weeks, when compared to pre-trial PT levels. Control rats showed no significant change in APTT levels over time. The rats fed abalone, however, showed significant differences in APTT levels. Specifically, when pre-trial APTT levels were compared with 4-week levels, and when 1-week levels were compared with 4-week levels, the differences attained statistical significance. These results indicate that an abalone-supplemented diet may inhibit blood coagulation in normal rats. The results of this study prove the inherent health value of abalone, and may encourage investment in the seafood industry. Future studies will explore other possible beneficial effects of abalone, apart from the anti-hypertension and anti-coagulant effects examined above.

Keywords

References

  1. Yoo, J.S. (1976) A illustrated book of the Korean shelfish. ILGi-SA, Seoul, Korea, p.36-37
  2. Yoo, S.K. (1979) A shallow seaculture. SERO Press, Seoul, Korea, p.309-368
  3. Yoon, J.A., Yu, K.W., Jun, W.J., Cho, H.Y., Son, Y.S., and Yang, H.C. (2000) Screening ofactivity in the extracts of edible seaweeds and optimization of condition. J. Korean Soc. Food Sci. Nutr., 29, 1098-1106
  4. Lee, Y.S., Kim, D.S., Ryu, B.H. and Lee, S.H. (1992) Antitumor and immunomodulating effects of seaweeds toward sarcoma-180 cell. J. Korean Soc. Food Nutr., 21, 544-550
  5. Cho, K.J., Lee, Y.S. and Ryu, B.H. (1992) Antitumor effect and immunology activity oftoward sarcoma-180. J. Korean Fish. Soc., 23, 345-352
  6. Kim, D.S. and Park, Y.H. (1985) Uronic acid composition, block structure and some related properties of alginic acid. J. Korean Fish Soc., 18, 29-36
  7. Colliec, S., Fischer, A.M., Tapon-Bretaudiere, J., Boisson, C., Durand, P. and Jozefonvicz, J. (1991) Anticoagulant properties of a fucoidan fraction. Thromb. Res., 64, 143-154 https://doi.org/10.1016/0049-3848(91)90114-C
  8. http://shop.suhyup.co.kr/cont_efct_html/b2c21085.html
  9. Chushman, D.W. and Cheung, H.S. (1971) Spectrophotometry assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacol., 20, 1673-1648
  10. You, E.J., Lim, H.S., Park, K.O. and Choi, M.R. (2005) Cytotoxic, antioxidative and ACE inhibiting activities of Dolsan leaf mustard juice (DLMJ) treated with Lactic acid Bacteria. Biotech. Bioprocess Eng., 10, 60-66 https://doi.org/10.1007/BF02931184
  11. Kim, Y.M., Do, J.R., In, J.P. and Park, J.H. (2005) Angiotensin converting enzyme (ACE) inhibitory activities of Laver (Porphyra tenera) protein hydrolysates. J. Korean Soc. Food Sci. Nutr., 18, 11-18
  12. Lee, S.E., Bang, J.G., Song, J., Seong, N.S., Park, H.U., Jeong, H.G., Kim, G.S. and An, T.J. (2004) Inhibitory activity on angiotensin converting enzyme (ACE) of Korean medicinal Herb. Korean J Med. Crop Sci., 1, 73-78
  13. Dennis, B.H., Haynes, S.G., Anderson, J., Liu-Chi, S., Hosking, J.D. and Rifkind, B.M. (1985) Nutrient intakes among selected North American populations in the lipid researchprevalence study: Composition of energy intake. Am. J. Clin. Nutr., 41, 312-329 https://doi.org/10.1093/ajcn/41.2.312
  14. Labarthe, D. (1998) Hypertension. In: Wallace RB, editor. Maxcy-Rosenau-Last Public Health & Preventive Medicine. 14th ed., Appleton & Lange, Stanford, U.S.A
  15. Williams, A.G., Rayson, M.P., Jubb, M., World, M., Woods, D., Hayward, M., Martin, J., Humphries, S.E. and Montgomery, H.E. (2000) The ACE gene and muscle performance. 403, 614-622
  16. Jaspard, E., Wei, L. and Alhenc-Gelas, F. (1993). Differences in the properties andspecificities of the two active sites of angiotensin I-converting enzyme (kininase II). Studies with bradykinin and other natural peptides. J. Biol. Chem., 268, 9496-9503
  17. Richards, A.M., Wittert, G.A., Espiner, E.A., Yandle, T.G., Ikram, H., and Frampton, C. (1992) Effect of inhibition of endopeptidase-24.11 on responses to angiotensin II in human volunteers. Circulation Res., 71, 1501-1507 https://doi.org/10.1161/01.RES.71.6.1501
  18. Unger, T. (2002) The role of the renin-angiotensin system in the development ofdisease. Am. J. Cardiol., 89, 3A-10A
  19. Murphey, L., Vaughan, D. and Brown, N. (2003) Contribution of bradykinin to theeffects of ACE inhibitors. Eur. Heart J., 5, A37-A41 https://doi.org/10.1016/S1520-765X(03)90062-9
  20. Mojgan, S. (2001) Vasopeptidase inhibitors: an emerging class of cardiovascular Hospital Pharma., 8, 280-282
  21. Cho, Y.J., Cha, W.S., Bok, S.K., Kim, M.U., Chun, S.S., Choi, U.K., Kim, S.H. and Park, K.S. (2000) Production and separation of angiotensin converting enzyme inhibitor during Natto fermentation. J. Korean Soc. Food Sci. Nutr., 29, 737-742
  22. Shin, Z.I., Ahn, C.W., Nam, H.S., Lee, H.J. and Moon, T.H. (1995) Fractionation of angiotensinenzyme (ACE) inhibitory peptides from soybean paste. Korean J. Food Sci. Technol., 27, 230-234
  23. Park, D.C., Park, J.H., Gu, Y.S., Han, J.H., Byun, D.S., Kim, E.M., Kim, Y.M. and Kim, S.B. (2000) Effects of salted-fermented fish produces and their alternatives on angiotensinconverting enzyme inhibitory activity of Kimchi during fermentation. Food Sci. Biotechnol. 32, 920-927
  24. Cha, M.H. and Park, J.R. (2001) Isolation and characterization of the strain producingconverting enzyme inhibitor from soy sauce. J. Korean Soc. Food Sci., 30, 594-599
  25. Ahn, S.W., Kim, Y.G., Kim, M.H., Lee, H.Y. and Seong, N.S. (1999) Comparison of biological activities on Rehmannia radix and R. radix preparata produced in Korea. Korean J. Med. Crop Sci., 7, 257-262
  26. H.Y. and Song, K.B. (2002) Isolation an angiotensin converting enzyme inhibitor from Ixeris dentara Nakai. Food Sci. Biotechnol., 11, 136-139
  27. Kim, H.L., Kang, S.G., Kim, I.C., Kim, S.J., Kim, D.W., Ma, S.J., Gao, T., Li, H., Kim, M.J., Lee, T.H. and Ham, K.S. (2006) In vitro anti-hypertensive, antioxidant and anticoagulant activities of extracts from Haliotis discus hannai. J Korean Soc Food Sci Nutr., 35, 835-840 https://doi.org/10.3746/jkfn.2006.35.7.835
  28. Kweon, M.H., Park, M.K., Ra, K.S., Sung, H.C. and Yang, H.C. (1996) Screening of bloodpolysaccharides from edible olants. Agric. Chem. Biotechnol., 39, 159-164
  29. Ra, K.S., Lee, B.L., Lee, H.S. and Kweon, M.H. (1997) An anticoagulant polysaccharidefrom Ganoderma lucidum. Korean J. Food Nutr., 10, 375-381
  30. Koo, J.G., Choi, Y.S. and Kwak, J.K. (2001) Blood-anticoagulant activity of fucoidans from sporophylls of Undaria pinnatifida Laminaria religiosa, Hizikia fusiforme and Sargassum fulvellum in Korea. Korean Fish Soc., 34, 515-520
  31. Takashi, N. (1990) The Relationship between the molecular weight and the anticoagulant activity of two types of fucan sulfates from the brown seaweed ecklonia kurome. Agric. Biol. Chem., 55, 791-796