한국가금학회지 (Korean Journal of Poultry Science)

  • 제34권2호
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  • Pages.119-128
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  • 2007
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
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Phytase 첨가가 산란계의 생산성, 난질, 질소와 인의 배설량 및 회장 소화율에 미치는 영향

Effects of Dietary Phytase on Performance, Egg Quality, Excretion of N and P, and Ileal Digestibility in Laying Hens

  • HwangBo, J. (National Institute of Animal Science) ;
  • Ahn, J.H. (National Institute of Animal Science) ;
  • Chung, W.T. (National Institute of Animal Science) ;
  • Ohh, S.J. (Institute of Animal Science Resources, Kangwon National University) ;
  • Lee, H.J. (National Institute of Animal Science) ;
  • Kim, H.K. (National Institute of Animal Science) ;
  • Lee, S.U. (National Institute of Animal Science) ;
  • Hong, E.C. (National Institute of Animal Science)
  • 발행 : 2007.06.30
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초록

산란계의 생산성, 계란 품질, 질소와 인의 흡수량과 배설량 및 회장 소화율에 미치는 파이타제의 첨가 효과를 구명하고자 사료에 0, 300, 600 FTU/kg의 파이타제를 첨가하여 4주간 사양 시험 종료후 1주간 대사실험을 실시하였다. 공시동물은 산란율이 유사한 55주령 White Leghorn 산란계 162수$(1.6{\pm}0.3\;kg)$를 공시하였으며, 처리구는 첨가 수준에 따라 P0(대조구), P1(300 FTU/kg), P2(600 FTU/kg)의 3처리구로 나누고, 처리구당 6반복, 반복당 9수씩 완전임의 배치하였다. 산란율은 파이타제 첨가 수준에 따라 증가하였다. 계란 품질은 난중, 난각 두께, 하우유니트와 난황색 모두 처리구간에 차이를 보이지 않았으나(p>0.05), 난각 강도는 파이타제 처리구에서 4주째에 각각 3.86 $kg/cm^2$와 3.85 $kg/cm^2$로 나타나 파이타제 첨가에 따른 차이를 보였다(p<0.05). 질소 배설량과 흡수량은 처리구간에 차이가 없었으나(p>0.05), 인 배설량과 흡수량은 파이타제 첨가구에서 대조구에 비해 배설량이 크게 감소하고 흡수량은 증가하였다(p<0.05). 파이타제 첨가에 따른 영양소와 아미노산의 소화율은 조섬유, 조회분, 칼슘과 인, 필수 아미노산 중 lysine, methionine, phenylalanine, 비필수 아미노산 중 alanine, cystine, glutamic acid, glycine, tyrosine의 소화율을 유의적으로 증가시켰다(p<0.05). 이 실험 결과 산란계에 미생물유래 파이타제 급여는 산란율을 향상시키고, 인 배설량을 낮추며, 흡수량을 증가시키지만, 계란 품질에 미치는 영향이 없었고, 소화율에 대한 영향은 추후의 연구가 필요하다고 사료된다.

The effects of microbial phytase on laying performance, egg quality, and ileal digestibility of nutrients and amino acids were examined at three levels of phytase (0, 300, 600 unit/kg) in 55-wk-old White Leghorn for 4 weeks. Egg productivity tended to increase with supplemental phytase compared to that of control. Daily feed intake of hens fed phytase also increased. Egg shell thickness was not significantly different among the treatments. Haugh unit and yolk color were not statistically different. However, egg shell breaking strength was high at phytase treatment. Excretion and absorption of nitrogen were no difference among all treatments, but those of phosphorus was higher in the phytase treatment than control. The digestibility was high at crude fiber, crude ash, calcium and phosphorus in nutrients, at lysine, methionine and phenylalanine in essential amino acids, and alanine, cystine, glutamic acid, glycine and tyrosine in non-essential amino acids. In conclusion, supplemental microbial phytase in laying hens diet may help to improve egg production and to decrease P of feces. But, further studies were needed to investigate on the digestibility.

키워드

참고문헌

  1. Abelson PH 1999 A potential phosphate crisis. Science 283, 2015 https://doi.org/10.1126/science.283.5410.2015
  2. Association of Official Analytical Chemists 1990 Official methods of Analysis, 15th Ed. (Washington, VA, AO, AOAC)
  3. Boling SD, Douglas MW, Johnson ML, Xang X, Parsons CM, Koelkebeck KW, Zimmerman RA 2000 The effects of dietary available phosphorus levels and phytase on performance of young and older laying hens. Poult Sci 79, 224-230 https://doi.org/10.1093/ps/79.2.224
  4. Camus MC, Laporte JC 1976 Inhibition de la proteolyse pepsique en vitro par de ble. Role de l'acide phytique des issues. Ann Biol Biochem Biophys 16:719-729 https://doi.org/10.1051/rnd:19760509
  5. Carlos AB, Edwards HM 1998 The effects of 1,25-dihydroxycholecalciferol and phytase on the natural phytate phosphorus utilization by laying hens. Poult Sci 77:850-858 https://doi.org/10.1093/ps/77.6.850
  6. Ceylan N, Scheideler SE, Stilborn HL 2003 High available phosphorus corn and phytase in layer diets. Poult Sci 82: 789-795 https://doi.org/10.1093/ps/82.5.789
  7. Correll DL 1999 Phosphorus: a rate limiting nutrient in surface waters. Poult Sci 78:674-682 https://doi.org/10.1093/ps/78.5.674
  8. Denbow DM, Ravindran V, Kornegay ET, Yi Z, Hulet RM 1995 Improving phosphorus availability in soybean meal for broilers by supplemental phytase. Poult Sci 74:1831-1842 https://doi.org/10.3382/ps.0741831
  9. Duncan DB 1995 Multiple range and multiple F tests. Bio- metrics 11:1-42
  10. Francesch M, Broz J, Brufau J 2005 Effects of an experimental phytase on performance, egg quality, tibia ash content and phosphorus bioavailability in alying hens fed on maize- or barley-based diets. Br Poult Sci 46:340-348 https://doi.org/10.1080/00071660500127001
  11. Gong LM, Li DF, Wang FL, Huang DS 2003 The effect of the ratio of threonine to lysine on performance, carcass quality, nutrient digestibility and plasma biochemistry indexes in broilers. Pages 52-63 in Proc. 2003 Studies on Animal Nutrition and Metabolism. Beijing Agricultural Press, Beijing, China
  12. Gordon RW, Roland DA 1997 Performance of commercial laying hens fed various phosphorus levels, with and without supplemental phytase. Poult Sci 76:1172-1177 https://doi.org/10.1093/ps/76.8.1172
  13. Haugh RR 1937 The Haugh unit for measuring egg quality. US Poult Mag 43:552-573
  14. Jalal MA, Scheideler SE 2001 Effect of supplementation of two different sources of phytase on egg production parameters in laying hens and nutrient digestibility. Poult Sci 80:1463- 1471 https://doi.org/10.1093/ps/80.10.1463
  15. Jalal MA, Scheideler SE, Wyatt C 1999 Effects of phytase supplementation on egg production parameters and amino acid digestibilities. Poult Sci 78 (Suppl. 1), 74 (Abstract)
  16. Kerr MJ, Classen HL, Newkirk RW 2000 The effects of gastrointestinal tract micro-flora and dietary phytase on inositol hexaphosphate hydrolysis in the chicken. Poult Sci 79 (Suppl. 1), 11 (Abstract)
  17. Keshavarz K 2000 Nonphytate phosphorus requirement of laying hens with and without phytase on a phase feeding program. Poult Sci 79:748-763 https://doi.org/10.1093/ps/79.5.748
  18. Keshavarz K 2003 Effects of continuous feeding low-phosphorus diets with and without phytase during the growing and laying periods of two strains of leghorns. Poult Sci 82:1444-1456 https://doi.org/10.1093/ps/82.9.1444
  19. Kies AK, van Hemert KHF, Sauer WC 2001 Effect of phytase on protein and amino acid digestibility and energy utlization. World's Poult Sci 57:109-126 https://doi.org/10.1079/WPS20010009
  20. Liebert F, Htoo JK, Sunder A 2005 Performance and nutrient utilization of laying hens fed low-phosphorus corn-soybean and wheat-soybean diets supplemented with microbial phytase. Poult Sci. 84:1576-1583 https://doi.org/10.1093/ps/84.10.1576
  21. Lim HS, Namkung H, Paik IK 2003 Effects of phytase supplementation on the performance, egg quality, and phosphorus excretion of laying hens fed different levels of dietary calcium and nonphytate phosphorus. Poult Sci. 82:92-99 https://doi.org/10.1093/ps/82.1.92
  22. Lynch GL 1996 Phytase allows flexibility in laying hen diets. p12 Feedstuffs April 1
  23. Maenz DD, Engele-Scham CM, Newkirt RW, Classen HL 1999 Th effect of minerals and mineral chelators on the formation of phytase-resistant and phytase-susceptible forms of phytic acid in solution and in a slurry of canola meal. Anim Feed Sci Technol 77:177-192
  24. Mason VC 1984 Metabolism of nitrogen compound in the large gut{Emphasis on recent findings in the sheep and pig}. Proc Nutr Soc 43:45-53 https://doi.org/10.1079/PNS19840026
  25. McDowell LR 1992 Pages 49-50 in: Minerals in Animal and Human Nutrition. Academic Press Inc., San Diego, CA
  26. Mullaney EJ, Daly CB, Ullah AHJ 2000 Advances in phytase research. Adv Appl Microbiol 47:157-199 https://doi.org/10.1016/S0065-2164(00)47004-8
  27. National Research Council 1994 Nutrient Requirements of Poultry, nineth revised ed. National Academy Press, Washington, DC
  28. Ouyang J, Yan M, Kong D, Xu L 2006. A complete protein pattern of cellulase and hemicellulase genes in the filamentous fungus Trichoderma reesei. Biotechnol J 1(11):1266-74 https://doi.org/10.1002/biot.200600103
  29. Panda AK, Rama Rao SV, Raju MVLN, Bhanja SK 2005 Effect of microbial phytase on production performance of white leghorn layers fed on a diet low in non-phytate phosphorus. Br Poult Sci 46:464-469 https://doi.org/10.1080/00071660500191098
  30. Peter W, Jeroch H 1993 The effectiveness of microbial phytase addition to layer rations on maize and wheat basis. pp 206-209 In: Enzymes in Animal Nutrition. Proc 1st Symp C Wenk and M Boessinger, ed. Roche, Kartause Ittingen, Switzerland
  31. Ravindran V, Bryden WL, Lornegay ET 1995 Phytases: occurrence, bioavailability and implications in poultry nutrition. Poult Avian Biol Rev 6:125-143
  32. Sandberg AS, Larsen T, Sandstorm B 1993 High dietary calcium levels decrease colonic phytate degradation in pigs. J Nutr 123:559-566
  33. SAS 1999 SAS suer guide. release 6.11 edition. SAS Inst Inc Cary NC USA
  34. Scott TA, Kampen R, Silversides FG 1999 The effect of phosphorus, phytase enzyme, and calcium on the performance of layers fed corn-based diets. Poult Sci 78:1742-1749 https://doi.org/10.1093/ps/78.12.1742
  35. Scott TA, Kampen R, Silversides FG 2001 The effect of adding exogenous phytase to nutrient-reduced corn- and wheat-based diets on performance and egg quality of two strains of laying hens. Can J Anim Sci 81:393-401 https://doi.org/10.4141/A00-095
  36. Selle PH, Ravindran V, Caldwell RA, Bryden WL 2000 Phytate and phytase: sonsequences for protein utilisation. Nutr Res Rev 13:255-278 https://doi.org/10.1079/095442200108729098
  37. Sharpley A 1999 Agricultural phosphorus, water quality and poultry production: are they compatible? Poult Sci 78:660- 673 https://doi.org/10.1093/ps/78.5.660
  38. Simons PCM, Versteegh HAJ, Jongbloded AW, Kemme PA, Slump P, Bos KD, Wolters MGE, Beudeker RF, Verschoor GJ 1990 Improvement of P availability by microbial phytase in broilers nd pigs. Br J Nutr. 64:525-540 https://doi.org/10.1079/BJN19900052
  39. Singh M, Krikorian AD 1982 Inhibition of trypsin activity in vitro by phytate. J Agric Food Chem 30:799-800 https://doi.org/10.1021/jf00112a049
  40. Snow JL, Baker DH, Parsons CM 2004 Phytase, citric acid, and 1$\alpha$-hydroxycholecalciferol improve phytate phosphorus utilization in chicks fed a corn-soybean meal diet. Poult Sci 83:1187-1192 https://doi.org/10.1093/ps/83.7.1187
  41. Sohail SS, Roland DA 2000 Influence of phytase on calcium utilization in commercial layers. J Appl Poult Res 9:81-87 https://doi.org/10.1093/japr/9.1.81
  42. Um JS, Paik IK 1999 Effects of microbial phytase supplementation on egg production, eggshell quality, and mineral retention of laying hens fed different levels of phosphorus. Poultry Sci 78:75-79 https://doi.org/10.1093/ps/78.1.75
  43. van der Klis JD, Versteegh HAJ 1991 Ileal Absorption of Phosphorus in Lightweight laying Hens Using Microbial phytase and Various Calcium Contents in Laying Hens Feed. Spelderholt, Beekbergen, The Netherlands, Spelderholt Publication No. 562
  44. van der Klis JD, Versteegh HAJ, Simons PCM, Kies AK 1997 The efficacy of phytase in corn-soybean meal-based diets for laying hens. Poult Sci 76:1535-1542 https://doi.org/10.1093/ps/76.11.1535
  45. 김범철 허우명 황길순 김동섭 최광순 1995 소양호에서 인의 존재 형태별 분포에 관하여. 한국수육학회지 28:151
  46. 김상호 유동조 나재천 최철환 상병돈 이상진 이원준 류경선 2000 산란 생산성과 인 이용성에 대한 Microbial Phytase의 첨가 효과. I. 무기태인 수준이 다른 사료에 Microbial Phytase 첨가가 산란성 및 인 이용성에 미치는 영향. 한국가금학회지 27(1):19-23
  47. 한국사양표준(가금) 2002 농림부․ 농촌진흥청 축산연구소
  48. 허우명 김범철 안태석 이기종 1992 소양호 유역과 가두리로부터 인 부하량 및 인 수지(phosphorus baget), 한국수육학회지 25:207
  49. 홍종욱 김인호 김은주 권오석 이상환 2001 Phytase 첨가가 산란계의 체내 영양소 균형 및 생산성에 미치는 효과. 한국가금학회지 28(1):1-6

피인용 문헌

  1. Effect of Dietary Phytase on Growth Performance and Excreta Excretion of Broilers vol.38, pp.4, 2011, https://doi.org/10.5536/KJPS.2011.38.4.255