Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

High Throughput Screening on Angiogenesis Inhibitor and Promoter of Medicinal Plants using a Protein Microarray Chip

  • Published : 2007.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of angiogenesis inhibitor from the extract libraries of Korean and Chinese medicinal plants were investigated using a protein microarray chip. Protein chip was constructed by immobilization of integrin ${\alpha}_5{\beta}_1$ on protein chip base plates and employed far screening active extracts that inhibit the integrin-fibronectin interaction from the extract libraries. The 100 extracts of medicinal plants were obtained from extract bank of National Institute of Crop Science, RDA. The 14 extracts among 100 extract libraries were shown efficient inhibition activity for the interaction between integrin-fibronectin. The medicinal plants of 14 extracts were Vitex negundo var. incisa (Lam.) C.B. Clarke, Epimedium koreanum Nakai, Cedrela sinensis A. Juss, Ipomea aquatica Forsk, Schisandra chinensis Baill, Pulsatilla koreana Nakai, Paeonia lactiflora Pall. var.hortensis Makino, Oenothera odorata, Allium chinense, Allium victorialis var. platyphyllum MAKINO, Polygonatum odoratum Druce var. pluriflorum Ohwi, Hosta lancifolia, Agrimonia pilosa L. var. japonica Nakai and Potentilla chinensis SER. The Paeonia lactiflora, Oenothera, and Agrimonia pilosa from these 14 extracts libraries were shown strong inhibition activity of integrin ${\alpha}_5{\beta}_1$.

Keywords

References

  1. Albes De Paulo S, Teruszkin Balassiano I, Henriques Silva N, Oliveira Castilho R, Coelho Kaplan MA, Currie Cabral M, Da Costa Carvalho MG (2000) Chrysobalanus icaco L. extract for antiangiogenic potential observation. Inter. J. Molecula Med. 5:667-669
  2. Arekov P, Kukhtin A, Gemmell A, Voloshchuk S, Chupeeva V, Mirzabekov A (2000) Protein microchips: use for immunoassay and enzymatic reactions. Anal Biochem. 278:123-131 https://doi.org/10.1006/abio.1999.4363
  3. Brakenhielm E, Cao R, Cao Y (2001) Suppression of angiogenesis, tumor growth and wound healing by resveratrol, a natural compound in red wine and grapes. FASEB. 15:1798-1800 https://doi.org/10.1096/fj.01-0028fje
  4. Bulyk ML, Gentalen E, Lockhart DJ, Church GM (1999) Quantifying DNA-protein interactions by double-stranded DNA arrays. Nat Biotech. 17:573-577 https://doi.org/10.1038/9878
  5. Choi S, Kim KW, Choi JS, Han ST, Park YI, Lee SK, Kim JS, Chung MH (2002) Angiogenic activity of a-sitosterol in the ischaemia I reperfusion-damaged brain of Mongolian gerbil. Planta Med. 68:330-335 https://doi.org/10.1055/s-2002-26750
  6. Folkman J (1971) Tumor angiogenesis. New England Journal of Medicine. 285: 1182-1186 https://doi.org/10.1056/NEJM197111182852108
  7. Fotsis T, Pepper M, Adlercreutz H, Fleischmann G, Hase T, Montesano R, Schweigerer L (1993) Genistein, a dietaryderived inhibitor of in vitro angiogenesis. Proceedings of the National Academy of Sciences of the United states of America. 90:2690-2694
  8. Glinkowska G, Balan B, Sommer E, Demkow D, sokolnicka I, Strzelecka H, Skopinska RE (1997) The effect of phenolic compounds of poplar leaves extract on cutaneous angiogenesis reaction induced in mice by human mononuclear leukocytes. Acta Poloniae Pharmaceutica. 54: 151-154
  9. Gonzalez S, Alcaraz MV, Cuevas J, Perez M, Jaen P, AlvarezMon M, Villarrubia VG (2000) An extract of the fern Polypodium leucotomos (Difur) modulates Th1/Th2 cytokines balance in vitro and appears to exhibit anti-angiogenic activities in vivo: pathogenic relationships and therapeutic implications. Anticancer Research. 20:567-575
  10. Hisa T, Kimura Y, Takada K, Suzuki F, Takigawa M (1998) Shikonin, an ingredient of Lithospermum erythrorhizon, inhibits angiogenesis in vivo and in vitro. Anticancer Research. 18:783-790
  11. Joos TO, Schrenk M, Hopfl (2000) A microarray enzymebased immuno sorbent assay for autoimmune diagnostics. Electrophoresis. 21:2641-2650 https://doi.org/10.1002/1522-2683(20000701)21:13<2641::AID-ELPS2641>3.0.CO;2-5
  12. Juarez CP, Muino JC, Gulielmone H, Sambuelli R, Echenique JR, Hernandez M, Luna JD (2000) Experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors. European J. Ophthalmology. 10:51-59 https://doi.org/10.1177/112067210001000109
  13. Jung YD, Ellis LM (2001) Inhibition of tumour invasion and angiogenesis by epigallocatechin gallate (EGCG), a major component of green tea. International J. Exper. Pathol. 82: 309-316 https://doi.org/10.1046/j.1365-2613.2001.00205.x
  14. Kim MS, Lee YM, Moon EJ, Kim SE, Lee JJ, Kim KW (2000) Anti-angiogenic activity of torilin, asesquiterpent compound isolated from Torilis japonica. International J. Cancer. 87:269-275
  15. Kimura Y, Baba K, Okuda H (2000) Inhibitory effects of active substances isolated from Cassia garrettiana heartwood on tumor growth and lung metastasis in Lewis lung carcinoma-bearing mice. Anticancer Research. 20:2899-2906
  16. Kim Y, Kim SB, You YJ, Ahn BZ (2002) Deoxypodophyllotoxin, the cytotoxic and antiangiogenic component from Pulsatilla koreana. Planta Med. 68:271-274 https://doi.org/10.1055/s-2002-23140
  17. Kobayashi S, Miyamoto T, Kimura I, Kimura M (1995) Inhibitory effect of isoliquiritin, a compound in licorice roots, on angiogenesis in vivo and tube formation in vitro. Biol & Pharm Bull. 18:1382-1386 https://doi.org/10.1248/bpb.18.1382
  18. Lee YS, Kang DK, Chang SI, Han MH, Kang IC (2004) High-Throughput screening of novel peptide inhibitors of an integrin receptor from the hexapeptide library by using a protein microarray chip. J, Biomolecular Screening. 9:687-694 https://doi.org/10.1177/1087057104268125
  19. Lueking A, Horn M, Eickhoff H, Bussow K, Lehrach H, Walter G (1999) Microarrays for gene expression and antibody screening. Anal Biochem. 270:103-111 https://doi.org/10.1006/abio.1999.4063
  20. MacBeath G (1999) Printing small molecules as micro arrays and detecting protein-ligand interactions en masse. J. Am. Chem. Soc. 121:7967-7968 https://doi.org/10.1021/ja991083q
  21. Mochizuki M, Yoo YC, Matsuzawa K, Sato K, Saiki I, Tonooka S, Samukawa K, Azuma I (1995) Inhibitory effect of tumor metastasis in mice by saponins, ginsenoside-$Rb_2$, 20(R)- and 20(S)-ginsenoside-$Rg_3$, of red ginseng. Biol & Pharm Bull. 18:1197-1202 https://doi.org/10.1248/bpb.18.1197
  22. Morisaki N, Watanabe S, Tezuka M, Zenibayashi M, Shiina R, Koyama N, Kanzaki T, Saito Y (1995) Mechanism of angiogenic effects of saponin from Ginseng Raix rubra in human umbilical vein endothelial cells. British J. Pharmacol. 115:1188-1193 https://doi.org/10.1111/j.1476-5381.1995.tb15023.x
  23. Shibata S (2001) Chemistry and cancer preventing activities of ginseng saponins and some related triterpenoid compounds. J. Korean Medical Sci. 16(Sup):S28-S37 https://doi.org/10.3346/jkms.2001.16.S.S28
  24. Shukla A, Rasik AM, Jain GK, Shankar R, Kulshrestha DK, Dhawan BN (1999) In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica. J. Ethnnopharmacolgogy. 65:1-11 https://doi.org/10.1016/S0378-8741(98)00141-X
  25. Sridhar J, Akula N, Sivanesan D, Narasimhan M, Rathinavelu A, Pattabiraman N (2005) Identification of novel angiogenesis inhibitors. Bioorganic & Medicinal Chemistry Letters. 15:4125-4129 https://doi.org/10.1016/j.bmcl.2005.06.001
  26. Sukhanov S, Delafontaine P (2005) Protein chip-based microarray profiling of oxidized low density Iipoproteintreated cells. Proteomics. 5: 1274-1280 https://doi.org/10.1002/pmic.200400985
  27. Takaku T, Kimura Y, Okuda H (2001) Isolation of an antitumor compound from Agaricus blaei Murill and its mechanism of action. The J. Nutrition. 131:1409-1413 https://doi.org/10.1093/jn/131.5.1409
  28. Uetz P, Giot L, Cagney G (2000) A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature. 403:623-627 https://doi.org/10.1038/35001009
  29. Wang S, Zheng Z, Weng Y, Yu Y, Zhang D, Fan W, Dai R, Hu Z (2004) Angiogenesis and anti-angiogenesis activity of Chinese medicinal herbal extracts. Life Sci. 74:2467-2478 https://doi.org/10.1016/j.lfs.2003.03.005
  30. Yoon TJ, Yoo YC, Choi OB, Do MS, Kang TB, Lee SW, Azuma I, Kim JB (1995) Inhibitory effect of Korean mistletoe (Viscum album coloratum) extract on tumour angiogenesis and metastasis ofhaematogenous and non-haematogenous tumour cells in mice. Cancer Lett. 97:83-91 https://doi.org/10.1016/0304-3835(95)03956-W
  31. Ziauddin J, Sabatini DM (2001) Microarrays of cells expressing defined cDNAs. Science. 411:107-110
  32. Zhou JR, Gugger ET, Tanaka T, Guo Y, Blackburn GL, Clinton SK (1999) Soybean phytochemicals inhibit the growth of transplantable human prostate carcinoma and tumor angiogenesis in mice. The J. Nutrition. 129:1628-1635 https://doi.org/10.1093/jn/129.9.1628