Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Changes in Volatile Compounds of Green Tea during Growing Season at Different Culture Areas

녹차엽의 채취 시기와 재배지역에 따른 휘발성 향기 성분의 변화

  • 이주연 (경희대학교 식품생명공학과) ;
  • 왕려비 (태평양기술연구원) ;
  • 백주현 (태평양기술연구원) ;
  • 박승국 (경희대학교 식품생명공학과)
  • Published : 2007.06.30
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Abstract

Fresh tea leaves grown in Jeju Island and Jeonnam Province of South Korea were plucked and processed. Volatile compounds (VCs) were analyzed and identified with SPME-GC/GC-MS/GC-O. The VCs of green teas were classified into two major categories based on their aroma characteristics: the Greenish (Group I), and Floral (Group II) odorants. It was found that the VCs were decreased significantly in fresh tea leaves as they were plucked at the later stages of cultivation. The ratio of VCs responsible for Group I and Group II compounds was well-balanced in tea leaves plucked in May, but the balances were changed when the fresh leaves were processed. The major VCs of fresh tea leaves in Jeju and Jeonnam were n-hexanal, E-2-hexenal, Z-3-hexenal, myrcene, benzyl alcohol, linalool, and phenyl alcohol. Also, Jeju and Jeonnam tea leaves had different aroma composition. n-Heptanol, ${\beta}-pinene$, benzaldehyde, and ethyl salicylate were found in Jeju fresh tea leaves, and Z-3-hexenol, E-2-hexenol, and methyl n-heptanoate were detected in Jeju dry tea leaves. On the other hand, Z-linalool oxide and myrcene were found in Jeonnam dry tea leaves. The SPME-GC method showed high reproducibility (RSD, 7.4%) with no-artifact formation. In this study, optimum plucking period of tea leaves could be determined for production of high quality green tea with a well-balanced aroma and characteristic VCs in green tea according to growing areas.

본 연구에서는 녹차엽의 채취 시기와 재배지역에 따른 휘발성 향기성분의 종류, 차이, 그리고 변화를 비교하기 위하여 제주와 전남지역에서 재배한 차의 생엽을 시기별로 채취하였다. 녹차엽의 휘발성 향기 성분은 SPME를 사용하여 포집, 흡착하여 GC, GC-MS, GC-O, 그리고 retention time을 이용하여 분리된 성분을 분석, 동정하였다. 녹차엽의 휘발성분의 양은 수분함량을 고려한 건조함량을 기준으로 정량하였다. 수분함량은 생엽이 78-80%, 건조엽이 3.20-4.98% 정도였다. 휘발성 향기성분의 흡착에 사용되는 SPME 방법은 이전의 여러 방법에 비해 분석시간이 짧고 본래의 향이 아닌 방해성분의 생성이 적은 장점이 있다. 본 연구에서 사용한 SPME fiber는 7.4%의 높은 재현성을 가졌으며 표준물질(ethyl n-heptanoate)의 내부표준법에 의한 재현성 결과에서는 불안정한 peak area값(RSD 30.5-98.7%)을 가져 3.47%의 재현성 결과를 가진 외부표준법을 사용하였다. 시기에 따른 휘발성 향기 성분을 비교한 결과, 5월에 채취한 생엽의 휘발량이 가장 많았으며, Group I과 Group II 향기특성의 함량비율이 균형적이었다. 휘발량이 가장 많았던 5월에 채취한 생엽을 제주와 전남의 재배지역으로 구분하여 비교한 결과, 주 성분은 Z-3-hexenal, linalool, E-2-hexenol, n-hexanal, Z-linalool oxide, myrcene, benzyl alcohol이었다. 이러한 결과는 채엽 시기가 녹차의 향기 특성에 영향을 주며, 채엽 시기가 같더라도 지역에 따라 향기가 다른 녹차가 제조될 수 있다는 것을 나타낸다. 본 연구는 녹차를 지역에 따라 구분하는 데에도 도움이 될 것으로 예상된다.

Keywords

References

  1. Baptista JAB, Tavares JFDP, Carvalho RCB. Comparison of catechins and aromas among different green teas using HPLC/SPME-GC. Food Res. Int. 31: 729-736 (1998) https://doi.org/10.1016/S0963-9969(99)00052-6
  2. Togari N, Kobayashi A, Aishima T. Pattern recognition applied to gas chromatographic profiles of volatile components in three tea categories. Food Res. Int. 28: 495-502 (1995) https://doi.org/10.1016/0963-9969(95)00029-1
  3. Kawakami M, Ganguly SN, Banerjee J, Kobayashi A. Aroma composition of Oolong tea and black tea by brewed extraction method and characterizing compounds of Darjeeling tea aroma. J. Agr. Food Chem. 43: 200-207 (1995) https://doi.org/10.1021/jf00049a037
  4. Choi SH, Chung DS, Jea SJ. A comparison of volatile aroma components in high grade Korean, Chinese, and Japanese green tea. J. Korean Home Eco. Assoc. 43: 33-40 (2005)
  5. Kumazawa K, Masuda H. Identification of potent odorants on Japanese green tea (Sen-cha). J. Agr. Food Chem. 47: 5169-5172 (1999) https://doi.org/10.1021/jf9906782
  6. Horita H, Owuor PO. Comparison and characterization of volatile components of Kenyan clonal teas and various black teas from other producing areas of the world. Bulletin of the National Research Institute of Vegetables, Orn. Plants Tea 1B: 55-65 (1987)
  7. Ravichandran R, Parthiban R. The impact of processing techniques on tea volatiles. Food Chem. 62: 347-353 (1998) https://doi.org/10.1016/S0308-8146(97)00229-X
  8. Yamanishi T. Flavor of green tea. Jpn Agr. Res. Quart. 12: 205-210 (1978)
  9. Moon JH, Lee JK, Song BH, Park KH. Aroma of tea. J. Korean Tea Soc. 2: 147-161 (1996)
  10. Park JH, Kim YO, Jung JM, Seo JB. Effect on quality of panfired green tea at different pan-firing conditions. J. Bio-Environ. Control 15 : 90-95 (2006)
  11. Yano M, Okada K, Kubota K, Kobayashi A. Studies on the precursors of monoterpene alcohols in tea leaves. Agr. BioI. Chem. Tokyo 54: 1023-1028 (1990) https://doi.org/10.1271/bbb1961.54.1023
  12. Mahanta PK, Tamuli P, Bhuyan LP. Changes of fatty acid content, lipoxygenase activities and volatiles during black tea manufacture. J. Agr. Food Chem. 41: 1677-1683 (1993) https://doi.org/10.1021/jf00034a030
  13. Takeo T. Production of linalool and geraniol by hydrolytic breakdown of bound forms in disrupted tea shoots. Phytochemistry 2: 2145-2147 (1981)
  14. Kim HJ, Ryu SK, Roh JC, Lee SJ, Park SK. Changes in lipoxygenase activity and volatile compounds of fresh tea leaves during early growing season. J. Korean Soc. Agr. Chem. Biotechnol. 46: 23-27 (2003)
  15. Kato M, Shibamoto T. Variation of major volatile constituents in various green teas from southeast Asia. J. Agr. Food Chem. 49: 1394-1396 (2001) https://doi.org/10.1021/jf001321x
  16. Takei Y, Ishiwata K, Yamanishi T. Aroma components characteristic of spring green tea. Agr. BioI. Chem. Tokyo 40: 2151-2157 (1976) https://doi.org/10.1271/bbb1961.40.2151
  17. Yamaguchi K, Shibamoto T. Volatile constituents of green tea, gyokuro. (Camellia sinensis L. var Yabukita). J. Agr. Food Chem. 29: 366-370 (1981) https://doi.org/10.1021/jf00104a035
  18. Shimoda M, Shigematsu H, Shiratsuchi H, Osajima Y. Comparison of volatile compounds among different grades of green tea and their relations to odor attributes. J. Agr. Food Chem. 43: 1621-1625 (1995) https://doi.org/10.1021/jf00054a038
  19. Shimoda M, Shigematsu H, Shiratsuchi H, Osajima Y. Comparison of the odor concentrates by SDE and adsorptive column method from green tea infusion. J. Agr. Food Chem. 43: 1616-1620 (1995) https://doi.org/10.1021/jf00054a037
  20. Choi SH, Bae JE. The aroma components of green tea, the products of Mt. Chiri garden. J. Korean Soc. Food Nutr. 25: 478-483 (1996)
  21. Lee JG, Kwon YJ, Chang HJ, Kwang JJ, Kim OC, Choi YH. Volatile components of green tea (Camellia sinensis L. var. Yabukita) by purge and trap headspace sampler. Korean J. Food Nutr. 10: 25-30 (1997)
  22. Belardi RP, Pawliszyn J. The application of chemically modified fused silica fibers in the extraction of organics from water matrix samples and their rapid transfer to capillary columns. J. Water Pollut. Res. Can. 24: 179-191 (1989)
  23. Arthur CL, Killam LM, Buchholz KD, Pawliszyn J. Automation and optimization of solid-phase microextraction. Anal. Chem. 64: 1960-1966 (1992) https://doi.org/10.1021/ac00041a034
  24. Arthur CL, Pawliszyn J. Solid phase microextraction with thermal desorption using fused silica optical fibers. Anal. Chem. 62: 2145-2148 (1990) https://doi.org/10.1021/ac00218a019
  25. Roberts DD, Pollien P, Milo C. Solid-phase microextraction method development for headspace analysis of volatile flavor compounds. J. Agr. Food Chem. 48: 2430-2437 (2000) https://doi.org/10.1021/jf991116l
  26. Coleman WMI. A study of the behavior of Maillard reaction products analyzed by solid phase microextraction-gas chromatography-mass selective detection. J. Chromatogr. Sci. 34: 213-218 (1996) https://doi.org/10.1093/chromsci/34.5.213
  27. Peppard TL, Ramus SA. Use of Kovat's gas chromatographic retention indices in beer flavor studies. Am. Soc. Brew. Chem. 46: 26-30 (1988)
  28. Garruti DS, Franco MRB, Maria Aparecida AP da S, Janzantti NS, Alves GL. Assessment of aroma impact compounds in a cashew apple-based alcoholic beverage by GC-MS and GC-olfactometry. LWT-Food Sci. Technol. 39: 372-377 (2006)
  29. Moon JH, Park KH, Sakata K. Aroma formation mechanism of tea. J. Korean. Tea Soc. 2: 271-296 (1996)
  30. Choi SH. Studies on flavor components of commercial Korean green tea: Korean J. Food Sci. Technol. 23: 98-101 (1991)
  31. Morita K, Wakabayashi M, Kubota K, Kobayashi A, Rerath NL. Aglycone constituents in fresh tea leaves cultivated for green and black tea. Biosci. Biotech. Bioch. 58: 687-690 (1994) https://doi.org/10.1271/bbb.58.687
  32. Im Sl, Choi SH. Study on fishy off-flavor in porcine liver by GC-O. Korean J. Food Sci. Technol. 35: 353-358 (2003)