Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Comparison of the Anti-inflammatory Effects of the Extracts from Rubus coreanus and Rubus occidentalis

토종 복분자와 외래종 복분자 추출물의 항염증효과 비교

  • Yang, Hyun-Mo (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Lim, Soon-Sung (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Lee, Yeon-Sil (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Shin, Hyun-Kyung (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Oh, Yang-Seok (Department of Medical Genetics, Hallym University) ;
  • Kim, Jin-Kyung (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University)
  • 양현모 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 임순성 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 이연실 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 신현경 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 오양석 (한림대학교 의학유전학교실) ;
  • 김진경 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터)
  • Published : 2007.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The dried fruit of the Rubus coreanus, which is well-known in Korea and referred to as 'Bokbunja,' has been employed as a traditional medicine for centuries. This crude drug has been utilized in Korea for the management of impotence, spermatorrhea, enuresis, asthma, and allergic diseases. Our previous study demonstrated that the ethanol extracts of R. coreanus have anti-inflammatory effects. The principal objective of the present study was to conduct a comparison of the anti-inflammatory effects of the ethanol extracts of R. coreanus and R. occidentalis; here, we tested the unripe (URCE), half-ripened (HRCE), and ripened fruits (RCE) of R. coreanus, and the unripe (UROE), half-ripened (HROE), and ripened fruits (ROE) of R. occidentalis. We found that URCE, UROE, HRCE, and HROE reduced the production of nitric oxide and prostaglandin $E_{2}$ as well as pro-inflammatory cytokines in lipopolysaccharide-stimulated RAW264.7 murine macrophages. Interestingly, the R. coreanus extracts showed stronger inhibitory effects on the production of these inflammatory mediators than the R. occidentalis extracts.

본 연구의 목적은 수세기에 걸쳐 발기부전(impotence), 정액루(spermatorrhea), 유뇨증(enuresis), 천식(asthma) 및 알레르기 관련 질병의 치료제로 사용되고 있는 토종 복분자 (R. coreanus)와 외래종 복분자(R. occidentalis)의 항염증활성을 비교, 분석해 보고자 했다. 그 결과 토종 복분자의 에탄올 추출물이 외래종 복분자의 에탄올 추출물에 비교해 LPS 처리에 의한 NO의 생성을 현저히 억제시키는 것을 관찰 할 수 있었으며, NO와 $PGE_{2}$ 생합성효소인 iNOS와 COX-2 단백질의 발현 또한 억제시킴을 확인할 수 있었다. LPS 유도에 의한 $TNF-{\alpha}$와 IL-6의 생성억제 효과도 토종 복분자 에탄올 추출물이 외래종 복분자 추출물에 비해 탁월함이 관찰 되었다. 이러한 연구결과로 볼 때 토종 복분자 에탄올 추출물이 macrophage에 의해 생성되는 염증반응의 매개물질인 NO, $PGE_2$, 염증성 cytokine 등의 생성을 억제함으로 염증반응을 완화시켜 줄 것으로 판단된다. 차후 항염증활성을 갖는 복분자 추출물의 성분을 동정하는 연구가 더욱더 필요할 것으로 사료되며, 토종 복분자가 천연 항염증활성을 갖는 제품 개발에 있어 유용한 식물자원 원료로 사용될 수 있을 것으로 생각된다.

Keywords

References

  1. Bae GH. The Medicinal Plants of Korea, Kyohak Publishing Co., Seoul, Korea. p. 231 (2000)
  2. Cha HS, Lee MK, Hwang JB, Park MS, Park KM. Physicochemical characteristics of Rubus coreanus Miquel. J. Korean Soc. Food Sci. Nutr. 30: 1021-1025 (2001)
  3. Pang KC, Kim MS, Lee MW. Hydrolyzable tannins from the fruits of Rubus coreanum. Korean J. Pharmacogn. 27: 366-370 (1996)
  4. Lee MW. Phenolic compounds from the leaves of Rubus coreanum. Yakhak Hoeji 39: 200-204 (1995)
  5. Lee YA, Lee MW. Tannins from Rubus coreanum. Korean J. Pharmacogn. 26: 27-30 (1995)
  6. Bogs J, Downey MO, Harvey JS, Ashton AR, Tanner GJ, Robinson SP. Proanthocyanidin synthesis and expression of genes encoding leucoanthocyanidin reductase and anthocyanidin reductase in developing grape berries and grapevine leaves. Plant Physiol. 139: 652-663 (2005) https://doi.org/10.1104/pp.105.064238
  7. Koundouras S, Marinos V, Gkoulioti A, Kotseridis Y, van Leeuwen C. Influence of vineyard location and vine water status on fruit maturation of nonirrigated cv. Agiorgitiko (Vitis vinifera L.). Effects on wine phenolic and aroma components. J. Agr. Food Chem. 54: 5077-5086 (2006) https://doi.org/10.1021/jf0605446
  8. Watson R, Wright CJ, McBurney T, Taylor AJ, Linforth RS. Influence of harvest date and light integral on the development of strawberry flavour compounds. J. Exp. Bot. 53: 2121-2129 (2002) https://doi.org/10.1093/jxb/erf088
  9. Moon GS. Constituents and Uses of Medicinal Herbs. Ilweolseogak, Seoul, Korea. pp. 310-311 (1991)
  10. Wang SY, Lin HS. Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. J. Agr. Food Chem. 48: 140-146 (2000) https://doi.org/10.1021/jf9908345
  11. Liu Z, Schwimer J, Liu D, Greenway FL, Anthony CT, Woltering EA. Black raspberry extract and fractions contain angiogenesis inhibitors. J. Agr. Food Chem. 53: 3909-3915 (2005) https://doi.org/10.1021/jf048585u
  12. Stoner GD, Chen T, Kresty LA, Aziz RM, Reinemann T, Nines R. Protection against esophageal cancer in rodents with lyophilized berries: potential mechanisms. Nutr. Cancer 54: 33-46 (2006) https://doi.org/10.1207/s15327914nc5401_5
  13. Chang YH, Lee ST, Lin WW. Effects of cannabinoids on LPS stimulated inflammatory mediator release from macrophages: involvement of eicosanoids. J. Cell Biochem. 81: 715-723 (2001) https://doi.org/10.1002/jcb.1103
  14. Korhonen R, Lahti A, Kankaanranta H, Moilanen E. Nitric oxide production and signaling in inflammation, Curro Drug Targets Inflamm. Allergy 4: 471-479 (2005)
  15. Raison LC, Capuron L, Miller AH. Cytokines sing the blues: inflammation and the pathogenesis of depression. Trends Immunol. 27: 24-31 (2006) https://doi.org/10.1016/j.it.2005.11.006
  16. Bogdan C. Nitric oxide and the immune response. Nat. Immunol. 2: 2907-2916 (2001)
  17. Laskin DL, Pendino KJ. Macrophages and inflammatory mediators in tissue injury. Annu. Rev. Pharmacol. 35: 655-677 (1995) https://doi.org/10.1146/annurev.pa.35.040195.003255
  18. Wu G, Morris SM. Arginine metabolism: nitric oxide and beyond. Biochem. J. 336: 1-17 (1998) https://doi.org/10.1042/bj3360001
  19. Harris SG, Padilla J, Koumas L, Ray D, Phipps RP. Prostaglandins as modulators of immunity. Trends Immunol. 23: 144-150 (2002) https://doi.org/10.1016/S1471-4906(01)02154-8
  20. Smith WL, Meade EA, DeWitt EL. Pharmacology of prostaglandin endoperoxide synthase isozymes 1 and 2. Ann. NY. Acad. Sci. 714: 136-142 (1994) https://doi.org/10.1111/j.1749-6632.1994.tb12037.x
  21. DuBois RN, Tsujii M, Bishop P. Cloning and characterization of a growth factor-inducible cyclooxygenase gene from rat intestinal epithelial cells. Am. J. Physiol. 266: G822-827 (1994)
  22. Park JH, Oh SM, Lim SS, Lee YS, Shin HK, Oh YS, Choe NH, Yoon PJH, Kim JK. Induction of heme oxygenase-1 mediates the anti-inflammatory effects of the ethanol extract of Rubus coreanus in murine macrophages. Biochem. Bioph. Res. Co. 351: 146-152 (2006) https://doi.org/10.1016/j.bbrc.2006.10.008