Studies on Functional Salt Fortified with Seaweed Components

해조성분 강화 기능성소금에 대한 연구

  • Published : 2007.04.30

Abstract

In an attempt to develop functional salts having beneficial health effects, we experimentally prepared three functional salts by fortification with soluble seaweed minerals (Hizikia mineral salt, HMS), fucoidan (fucoidan salt, FS) and laver extracts (laver salt, LS). To characterize the functional salts, their physicochemical properties and in vitro functionalities, such as pH, color, mineral composition, solubility, oxidation-reduction potential, sensory properties, angiotesin converting enzyme (ACE) inhibitory activity, and bile acid binding capacity were investigated. The functional salts revealed slightly lower NaCl concentrations, but showed a variety of pH values compared with conventional table salt. The pH values of HMS, FS, and LS were 11.3, 6.8, and 6.5, respectively. The oxidation-reduction potentials (ORP) of the functional salts varied from -229 mV to 38 mV, significantly lower than refined salt. The functional salts were significantly darker in color than refined salt, and the mineral composition of HMS was considerably enriched compared to refined salt, particularly in potassium ion. As a result of the sensory evaluation, FS and LS were comparatively palatable in saltiness, pungency, bitterness, and overall acceptance compared with refined salt. It was also found that one functional salt had ACE inhibitory activity (54.8% in LS) and another had bile acid binding capacity (80.7% in FS).

해조성분 강화 기능성소금 3종(Hizikia mineral salt, fucoidan salt, laver salt)을 제조하여 일반적 품질특성 및 무기성분 조성, 용해도, 관능특성 및 ACE 저해활성과 bile acid 결합력을 알아보았다. 수분함량은 Hizikia mineral salt의 경우 대조시료와 유의한 차이가 없었으나 fucoidan salt와 laver salt는 상대적으로 높은 수분함량을 나타내었다. 염도는 해조성분 강화 기능성소금 3종 모두 대조시료 보다는 약간 낮은 값을 나타내었고 pH는 Hizikia mineral salt의 경우 11.34로 다소 높은 값을, fucoidan salt와 laver salt은 6.88과 6.53으로 중성에 가까운 값을 나타내었으며, ORP는 -229 mV에서 38 mV로 월등히 낮은 값을 나타내었다. 기능성 소금의 L, a, b 값은 모두 정제염 보다는 약간 어두운색을 나타내었다. 무기물 함량은 Hizikia mineral salt에서 혈압 저하에 효과가 있는 무기성분인 $K^{+}$ 함량이 다량 함유되어 있었으며 용해도는 Hizikia mineral salt가 다소 떨어지는 경향을 나타내었으나 큰 차이는 나타나지 않았다. 관능특성을 알아본 결과 $Na^{+}$ 이외의 미네랄 성분을 다량 함유하고 있는 Hizikia mineral salt를 제외한 나머지 fucoidan salt, laver salt에서는 색을 제외한 모든 항목에서 높은 점수를 받아 조미기능성 염으로서도 사용가치가 기대되었다. 또한, laver salt는 54.8% 수준의 ACE 저해 활성값을 나타내었으며, fucoidan salt는 54.1-80.7% 수준의 높은 bile acid 결합특성을 각각 나타냄으로서 콜레스테롤 대사 및 혈압조절 기능성을 갖고 있는 것으로 사료되었다.

Keywords

References

  1. Kim DH, Lee SB, Rbim JW, Characteristics of seaweed salt prepared with seaweeds. Korean J. Food Sci. Technol. 36: 937-942 (2004)
  2. The National Statistical Office of the Economic Planning Board. 1990th The origin of death. p. 1 (1991)
  3. Kim HS, Yu CH. The effect of Ca supplementation on the metabolism of sodium and potassium and blood pressure in college women. Korean J. Nutr. 30: 32-39 (1997)
  4. Cho KH, Park Ma, Kim ES. Differences between estimated and analyzed contents of sodium and potassium in the salt-restricted diet. J. Korean Soc. Food Nutr. 25: 406-414 (1996)
  5. Park TS, Lee GY. A study on the sodium and potassium intakes and their metabolism of university students in Korea. Korean J. Nutr. 18: 201-208 (1985)
  6. Kim YS, Bake HY. Measurement of Na intake in Korean adult females. Korean J. Nutr. 20: 341-349 (1987)
  7. The Korean Nutrition Society. Korean Recommended Intake. 6th ed. pp. 134-136 (1995)
  8. Kang YJ, Ryu KT, Kim HS. Preparation of cellular liquid from brown seaweeds for functional tonic products. J. Korean Soc. Food Nutr. 25: 94-103 (1996)
  9. Jeong K. The production system of total salt at guom village in Cheju island. Geographical J. Korean 32: 87-104 (1998)
  10. Jo EJ, Shin DH. Study on the chemical compositions of sundried, refined, and processed salt produced in Chonbuk area. J. Food Hyg. Saf. 13: 360-364 (1998)
  11. Park JW, Kim SJ, Kim SH, Kim BH, Kang SK, Nam SH, Jung ST. Determination of mineral and heavy metal contents of various salts. Korean J. Food Sci. Technol. 32: 1442-1445 (2000)
  12. Ha JO, Park KY. Composition of mineral contents and external structure of various salts. J. Korean Soc. Food Sci. Nutr. 27: 413-148 (1998)
  13. Kim YM, Do JR, In JP, Park JH. Angiotensin converting enzyme (ACE) inhibitory activities of laver (Porphyra tenera) protein hydrolysates. Korean J. Food Nutr. 18: 11-18 (2005)
  14. Koo JG, Jo KS, Do JR, Woo SJ. Isolation and purification of fucoidans from Laminaria religiosa and Undaria pinnatifida in Korea. J. Korean Fish Soc. 28: 227-236 (1995)
  15. AOAC. Official Methods of Analysis, 17th ed. Method 995.04. Association of Official Analytical Chemists. Washington, DC, USA (2000)
  16. Bin JH, Kim HD, Ryu BB. Anticomplementary activities of rhamnan sulfate extracted from monostroma nitidum. Korean J. Food Nutr. 9: 490-495 (1996)
  17. Blakeney AB, Harris PJ, Henry RT, Stone BA. A simple and rapid preparation of alditol acetate for monosaccharide analysis. Carbohyd. Res. 113: 291-299 (1983) https://doi.org/10.1016/0008-6215(83)88244-5
  18. AOAC. Official Methods of Analysis, 17th ed. Method 934.01. Association of Official Analytical Chemists. Washington, DC, USA (2000)
  19. The Ministry of Health and Welfare. Food Standard Code. Seoul, Korea. pp. 173-177 (2000)
  20. Kim KO, Kim SS, Sung NK, Lee YU. A sensory Test and Application. Sin Kwang, Seoul, Korea. pp. 207-225 (1993)
  21. Chshman DW, Cheung HS. Spectrometric assay and proterties of angiotensin coverting enzyme of rabbit lung. Biochem. Phamacal. 20: 1637-1648 (1971) https://doi.org/10.1016/0006-2952(71)90292-9
  22. You BJ, Im YS, Jeong IH, Lee KH. Effect of extraction conditions on bile acids inding capacity in vitro of alginate extracted from sea tangle (Laminaria spp.). J. Korean Fish. Soc. 30: 31-38 (1997)
  23. SAS Institute. Inc. SAS User's Guide. Statistical Analysis Systems Institute, Cary, NC, USA (1998)
  24. Moon DS, Kim HJ, Shin PK, Jung DH. Characteristics of chemical contents of horizontal spray salts from deep ocean water. J. Korean Fish. Soc. 38: 65-69 (2005) https://doi.org/10.5657/kfas.2005.38.1.065
  25. Huh K, Kim MH, Kim MG, Song IS. Salts safety in the side of food sanitation. J. East Asian Diet. Life 9: 386-387 (1999)
  26. Kim DH, Lee SB, Rhim JW, Characteristics of seaweed salts prepared with various seaweeds. Korean J. Food Sci. Technol. 35: 62-66 (2003)
  27. Ha JO, Park KY. Comparison of autooxidation rate and cornutagenic effect of effect of different kinds of salt. J. Korean Asso. Cancer Prev. 4: 44-51 (1999)
  28. Maurice ES, Vernon RY. Nutrition and diet in hypertention. Vol II. p. 1272. In: Modern Nutrition in Health and Disease. 7th ed. Lea & Febiger, PA, USA. (1988)
  29. Kim DH, Lee SB, Rhim JW. Characteristics of seaweed salts prepared with seaweeds. Korean J. Food Sci. Technol. 36: 937-942 (2004)
  30. Kunio S. Purification and identification of angiotensin-converting enzyme inhibitors from the red alga Porphyra yezoensis. J. Marin Biotechnol. 6: 163-167 (1998)
  31. Jung KJ, Jung BM, Kim SB. Effect of porphyran isolated from laver, Porphyra yezoensis, on lipid metabolism in hyperlipidemic and hypercholesterolemic rats. Korean J. Food Sci. Technol. 33: 633-640 (2001)
  32. Kim SJ, Moon JS, Kang SG, Jung ST. Exraction of perphyran from decolored laver. Korean J. Food Sci. Technol. 35: 1017-1021 (2003)
  33. Maruyama S, Nakagomi K, Tomizuka N, Suzuki H. Angiotensin converting enzyme inhibitor derived from an enzymatic hydrolysate of casein. Agr. Biol. Chem. Tokyo 49: 1405-1410 (1985) https://doi.org/10.1271/bbb1961.49.1405
  34. Maruyama S, Mitachi H, Tankata H, Tomizuka N, Suzuki H. Studies on the active site and antihypertensive activity of angiotensin converting enzyme inhibitors derived from casein. Agr. Biol. Chem. Tokyo 51: 1581-1586 (1987) https://doi.org/10.1271/bbb1961.51.1581
  35. Kinoshita E, Yamakoshi J, Kikuchi M. Purification and identification of an angiotensin-converting enzyme inhibitor from soy sauce. Bio. Oiotech. Biochem. 57: 1107-1110 (1993) https://doi.org/10.1271/bbb.57.1107
  36. Kang DG, Lee YS, Kim HJ, Lee YM, Lee HS. Angiotension coverting enzyme inhibitory phenylpropanoid glycosides from clerodendron trichotomum. J. Ethnopharmacol. 89: 151-154 (2003) https://doi.org/10.1016/S0378-8741(03)00274-5
  37. Hwang JH. Angiotensin-converting enzyme inhibitory effect of doenjang fermented by B. subtillis SCB-3 isolated from meju, Korean traditional food. J. Korean Soc. Food Sci. Nutr. 26: 775-783 (1997)
  38. Lee JE. Salt and hypertension. Korean J. Nephrol. 11: 6 (1992)