Exposure Assessment of Total Aflatoxin in Foods

식품중 총 아플라톡신의 노출량 평가

  • Suh, Jung-Hyuk (Department of Food Evaluation, Korea Food & Drug Administration) ;
  • Sho, You-Sub (Department of Food Evaluation, Korea Food & Drug Administration) ;
  • Park, Seong-Soo (Seoul Regional of KFDA) ;
  • Choi, Woo-Jeong (Gyeongin Regional of KFDA) ;
  • Lee, Jong-Ok (Department of Food Evaluation, Korea Food & Drug Administration) ;
  • Kim, Hee-Yun (Gyeongin Regional of KFDA) ;
  • Woo, Gun-Jo (Department of Food Evaluation, Korea Food & Drug Administration) ;
  • Oh, Keum-Soon (Department of Food Evaluation, Korea Food & Drug Administration)
  • 서정혁 (식품의약품안전청 식품평가부) ;
  • 소유섭 (식품의약품안전청 식품평가부) ;
  • 박성수 (서울지방식품의약품안전청) ;
  • 최우정 (경인지방식품의약품안전청) ;
  • 이종옥 (식품의약품안전청 식품평가부) ;
  • 김희연 (경인지방식품의약품안전청) ;
  • 우건조 (식품의약품안전청 식품평가부) ;
  • 오금순 (식품의약품안전청 식품평가부)
  • Published : 2007.02.28

Abstract

A survey of total aflatoxin levels was conducted on 565 food samples (cereals, nuts, etc) collected in commercial markets. The determination of aflatoxins ($B_{1}$, $B_{2}$, $G_{1}$ and $G_{2}$) was performed using HPLC with fluorescence detector. The Limit of Detections (LODs) of the B group and G group were 0.05 ng/g and 0.07 ng/g, respectively. In addition, recoveries of rice, peanut butter, and red pepper flour were satisfactory. Total aflatoxin was detected 27 samples(4.8%) out of 565 samples. Incidence ratios in cereals, nuts, processed products, and other foods were 0.2, 0.4, 3.0 and 1.2%, respectively, but aflatoxin was not detected in pulse and dried fruits. The daily intake of total aflatoxin using food intakes was 0.04 ng/kg bw/day.

HPLC/FLD를 이용하여 곡류, 견과류 등 총 565건을 대상으로 총 아플라톡신 함량을 조사하여 노출량을 평가하였다. B와 G 그룹의 검출한계는 각각 0.05, 0.07 ng/g이였고, 쌀, 땅콩버터 및 고춧가루에 대한 아플라톡신($B_{1}$, $B_{2}$, $G_{1}$$G_{2}$)의 회수율은 양호한 수준이었다. 총 아플라톡신은 전체 시료 565건 중 27건(4.77%)이 검출되었으며, 검출빈도는 곡류 0.17%, 견과류 0.35%, 가공식품 3.01%, 기타식품 1.24%을 보여주었고, 두류 및 건조과일류는 검출되지 않았다. 식이섭취량을 고려한 총 아플라톡신의 1일 1인당 평균섭취량은 0.04 ng/kg bw/day이였다.

Keywords

References

  1. IARC. Monograph on the evaluation of carcinogenic risk to humans (Lyon: IARC). Vol. 82, p. 171(2002)
  2. European Commission. Commission Regulation. Available from: http://europa.eu.int/eur-Iex/en/consleg/pdf/2001/en_2001R0466_do_001.pdf. Accessed Dec.12,2005
  3. Food and Drug Administration. Action levels for poisonous or deleterious substances in human food and animal feed. Available from: http://www.cfsan.fda.gov/~lrd/fdaact.html#afla. Accessed Dec.7, 2005
  4. Codex. Maximum level and sampling plan for total aflatoxins in peanuts intended for further processing. Available from: http://www.codexalimentarius.net/download/standards/335/CXS_209e.pdf. Accessed Mar. 6, 2006
  5. Food and Drug Administration. Food code. Korea Food Industry Association, Namhyung-Munhwa, Seoul, Korea. p. 152(2005)
  6. Ministry of Health Labour and Welfare of Japan. Available from: http://www.mhlw.go.Jp/index.html. Accessed Feb. 23, 2006
  7. Ministry of Health and Welfare. Report on 2001 National Health and Nutrition Survey I. Ministry of Health and Welfare, Seoul, Korea (2003)
  8. Lee MC, Hong MG, Park KS, Choi DM, Lim MH, Lee SR. Procedures in establishing residue limits of pesticides on food crops in Korea. Korean J. Food Sci. 37:685-694(2005)
  9. Chan D, MacDonald SJ, Boughtflower V, Brereton P. Simulaneous determination of aflatoxins and ochratoxin A in food using a fully automated immunoaffinity column clean-up and liquid chromatography-fluorescence detector. J. Chromatogr. A 1059: 13-16(2004) https://doi.org/10.1016/j.chroma.2004.09.096
  10. Candlish AAG, Pearson SM, Aidoo KE, Smith JE, Kelly B, Irvine H. A survey of ethnic foods for microbial quality and aflatoxin content. Food Addit. Contam. 18: 129-136(2001) https://doi.org/10.1080/02652030010021404
  11. Abdulkadar AHW, AI-Ali A, AI-Jedah J. Aflatoxin contamination in edible nuts imported in Qatar. Food Control 11: 157-160(2000) https://doi.org/10.1016/S0956-7135(99)00088-2
  12. Erdogan A. The aflatoxin contamination of some pepper types sold in Turkey. Chemosphere 56: 321-325(2004) https://doi.org/10.1016/j.chemosphere.2004.02.020
  13. Zinedine A, Brera C, Elakhdari S, Catano C, Debegnach F, Angelini S, De Santis B, Faid M, BenJemlih M, Minardi V, Miraglia M. Natural occurrence of mycotoxins in cereals and spices commercialized in Morocco. Food Control 17:868-874(2006) https://doi.org/10.1016/j.foodcont.2005.06.001
  14. Park JW, Kim EK, Kim YB. Estimation of the daily exposure of Koreans to aflatoxin B1 through food consumption. Food Addit. Contam. 21: 70-75(2004) https://doi.org/10.1080/02652030310001622782
  15. JECFA. Forty-ninth meeting of the Joint FAO/WHO Expert Committee on Food Additives. Safety Evaluation of Certain Food Additives and Contaminants in Food: Aflatoxins. WHO Food Additives Series, 40 (Geneva: WHO), pp. 359-469(1998)
  16. Thuvander A, Moller T, Enghardt Barbieri H, Jansson A, Salomonsson AC, Olsen M. Dietary intake of some important mycotoxins by the Swedish population. Food Addit. Contam. 18:696-706(2001) https://doi.org/10.1080/02652030121353
  17. International Programme on Chemical Safety World Health Organization. Safety Evaluation of Certain Food Addititives and Contaminants (Aflatoxins). Available from: http://www.inchem.org/documents/jecfa/jecmono/v040je16.htm. Accessed Feb. 20, 2005
  18. European Commission, Rapid Alert System Food and Feed. Available from: http://ec.europa.eu/foocl/food/rapidalert/index_en.htm. Accessed Dec. 30, 2005