YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Hepatoprotective Effects of the Water Extract of Protaetia brevitarsis Larva Against Carbon Tetrachloride-Induced Liver Injury in Rats

사염화탄소에 의해 유도된 흰쥐의 간장해에 미치는 지잠의 보호효과

  • Published : 2007.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Hepatoprotective effects of the water extract of Protaetia brevitarsis larva (PB) was investigated in carbon tetrachloride ($CCI_{4}$) treated male Sprague-Dawley rats. PB administration protected rats against ALT, AST and LDH elevations induced by $CCI_{4}$, as well as the severity of liver damage. PB recovered the decrease in serum level of high-density lipoprotein-cholesterol and the increase in serum level of low-density lipoprotein-cholesterol induced by $CCI_{4}$. In histopathological observation, massive fatty change and necrosis in the centrilobular area, degenerative change including pyknosis of nucleus and swelling of parenchymal cell induced by $CCI_{4}$ were clearly protected by PB. These histopathological findings paralleled with the serum biochemical results. The present results demonstrated that the water extract of PB may have the hepatoprotective effect against $CCI_{4}$-induced liver damage in vivo.

Keywords

References

  1. 신민교 : 원색임상본초학, 도서출판 영림사, p. 482 (1986)
  2. 김진일 : 한국곤충생태도감, 고려대학교 한국곤충연구소, Vol. III, pp. 45-78 (1998)
  3. 古川晴男, 長谷川仁, 奐谷禎一 : 原色昆蟲百科圖鑑, 集英社, pp. 573-574 (1977)
  4. 張芝利 : 中國經濟昆蟲誌 28책, 초시목, 中國科學院 中國動物誌編纂委員會, 科學出版社 (1984)
  5. 赤松金榜 : 和漢藥, 醫齒藥出版社, 東京, p. 950 (1970)
  6. 李時珍 : 本草綱目, 人民衛生出版社, 北京, pp. 2297-2298 (1981)
  7. 孫星衍 : 神農本草經, 聞名學社出版社, 台灣, 卷二, p. 30 (1985)
  8. 中藥大辭典 : 上海科學技術出版社, 第2卷, 東京, 小學館, p. 1389 (1985)
  9. Sherlock, S. and Dooley, J. S. : Disease of the Liver and Biliary System. 10th ed., pp. 303-384 (1997)
  10. Anthony, P. P., Ishak, K. G., Nayak, N. C., Poulsen, H. E., Scheuer, P. J. and Sobin, L. H. : The morphology of cirrhosis: definition, nomenclature, and classification. Bull. World Health Organ. 55, 521 (1977)
  11. Anthony, P. P., Ishak, K. G., Nayak, N. C., Poulsen, H. E., Scheuer, P. J. and Sobin, L. H. : The morphology of cirrhosis. Recommendations on definition, nomenclature, and classification by a working group sponsored by the World Health Organization. J. Clin. Pathol. 31, 395 (1978)
  12. Recknagel, R. O. : Carbon tetrachloride hepatotoxicity. Pharmacol. Rev. 19, 145 (1967)
  13. Rechnagel, R. O. and Glende, E. A. Jr. : Carbon tetrachloride hepatotoxicity: an example of lethal cleavage. CRC Crit. Rev. Toxicol. 2, 263 (1973)
  14. de Groot, H. and NoIl, T. : The crucial role of low steady state oxygen partial pressures in haloalkane free-radical-mediated lipid peroxidation. Possible implications in haloalkane liver injury. Biochem. Pharmacol. 35, 15 (1986)
  15. Moore, L., Davenport, R. G. and Landon, E. J. : Calcium uptake of a rat liver microsomal subceIlular fraction in response to in vivo administration of carbon tetrachloride. J. Biol. Chem. 251, 1197 (1976)
  16. Hofmann, A. F. and Popper, H. : Ursodeoxycholic acid for primary biliary cirrhosis. Lancet 2, 398 (1987)
  17. Campos, R., Garrido, A., Guerra, R. and Valenzuela, A. : Silybin dihemisuccinate protects against glutathione depletion and lipid peroxidation induced by acetaminophen on rat liver. Planta Med. 55, 417 (1989)
  18. 김덕현, 윤수홍 : 소시호탕이 사염화탄소에 의해 유도된 흰쥐의 간 장해에 미치는 영향. 한국위생과학학회지 4, 3 (1998)
  19. 윤수홍, 김덕현, 현선희, 이상규, 전태원, 정태천 : 랫드 일차 배양 간세포에서 사염화탄소의 독성에 대한 지잠 물추출물의 보호효과. 약학회지 50, 287 (2006)
  20. McComb, R. B. and Bowers, G. N. Jr. : Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum. Clin. Chem. 18, 97 (1972)
  21. Frings, C. S. and Dunn, R. T. : A colorimetric method for determination of total serum lipids based on the sulfophospho-vanillin reaction. Am. J. Clin. Pathol. 53, 89 (1970)
  22. Plaa, G. L. and Hewitt, W. R. : Detection and evaluation of chemicaIly induced liver injury in Principles and Methods of Toxicology (A. W. Hayes, ed.). 2nd ed. Raven Press, New York, pp. 599-628 (1989)
  23. Frimpong, N. A. and Lapp, J. A. : Effects of moderate alcohol intake in fixed or variable amounts on concentration of serum lipids and liver enzymes in healthy young men. Am. J. Clin. Nutr. 50, 987 (1989)
  24. 이지영, 이영주, 한준표 : 송화분 단백질이 사염화탄소를 투여한 흰쥐의 혈청에 미치는 효과. J. Korean Soc. Food Nutr. 25, 34 (1996)
  25. Rhoads, G. G., Gulbrandsen, C. L. and Kagan, A. : Serum lipoproteins and coronary heart disease in a population study of Hawaii Japanese men. N. Engl. J. Med. 294, 293 (1976)
  26. Gordon, T., Castelli, W. P., Hjortland, M. C., Kannel, W. B. and Dawber, T. R. : High density lipoprotein as a protective factor against coronary heart disease. The Framingham Study. Am. J. Med. 62, 707 (1977)
  27. Miller, G. J. and Miller, N. E. : Plasma-high-density-lipoprotein concentration and development of ischaemic heart-disease. Lancet 1, 16 (1975)
  28. Goldstein, J. L. and Brown, M. S. : The low-density lipoprotein pathway and its relation to atherosclerosis. Annu. Rev. Biochem. 46, 897 (1977)
  29. Takeda, Y., Ichihara, A., Tanioka, H. and Inoue, H. : The biochemistry of animal cells. I. The effect of corticoteroids on leakage of enzymes from dispersed rat liver cells. J. Biol. Chem. 239, 3590 (1964)
  30. Plaa, G. L. and Hewitt, W. R. : Quantative evaluation of indicies of hepatotoxicity in Toxicology of the Liver (D. Zakim and T. D. Boyer, Eds.). Raven Press, New York, pp. 103-120 (1982)
  31. Yoo, Y. C., Shin, B. H., Hong, J. H., Lee, J., Chee, H. Y., Song, K. S. and Lee, K. B. : Isolation of fatty acids with anticancer activity from Protaetia brevitarsis larva. Arch. Pharm. Res. 30, 361 (2007) https://doi.org/10.1007/BF02977619