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Characteristic Changes in Red Ginseng Fusion Cheonggukjang Based on Hydrolysis Conditions

홍삼 융합청국장의 가수분해 조건에 따른 특성변화

  • Lee, Myung-Hee (Dept. of Fermentation and Health Food, Kyongbuk College of Science) ;
  • Gu, Young-Ah (Keimyung Foodex Co., Ltd.) ;
  • Choi, Myung-Sook (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kwon, Joong-Ho (Dept of Food Science and Technology, Kyungpook National University) ;
  • Kim, In-Sun (Dept. of Biology, Keimyung University) ;
  • Jeong, Yong-Jin (Dept. of Food Science and Technology, Keimyung University and Keimyung Foodex Co., Ltd.)
  • 이명희 (경북과학대학 발효건강식품과) ;
  • 구영아 ((주)계명푸덱스) ;
  • 최명숙 (경북대학교 식품영양학과) ;
  • 권중호 (경북대학교 식품공학과) ;
  • 김인선 (계명대학교 생물학과) ;
  • 정용진 (계명대학교 식풍가공학과 및 (주)계명푸덱스)
  • Published : 2007.08.30

Abstract

Changes in red ginseng fusion cheonggukjang properties under various hydrolytic conditions were investigated for its possible application to different types of food products. Among the four types of protease that were analyzed, protease (KMF -G) produced the highest hydrolysis rate, calcium binding capacity, and total phenolic compound content. In addition, the highest fibrinolytic activity and ACE inhibitory activity were also exhibited at 87.10 units and 67.17%, respectively. Among a number of different protease concentrations, a 0.02% concentration of protease (KMF-G) was found to be appropriate for the purposes of the study. The best results for red ginseng cheonggukjang hydrolysis were observed at the 60 and 90 min intervals. However, there was not a significant difference between the results at the two time points. The unpleasant odor and bitter taste associated with red ginseng fusion cheonggukjang improved with hydrolytic activity exceeding 60 min. Thus, the optimal hydrolysis time was determined to be 60 min. The total ginsenoside content of red ginseng cheonggukjang was 9.197 mg/g and the hydrolysate content was 11.707 mg/g. Based on the results, it was determined that the addition of 0.02% protease (KMF -G) and treatment for 60 min are the optimal hydrolytic conditions for red ginseng cheonggukjang to improve its biochemical characteristics, including fibronolytic activity and ACE inhibitory activity.

홍삼 융합청국장을 다양한 식품소재로 활용하고자 가수 분해 조건에 따른 특성변화를 조사하였다. 그 결과 4종류의 protease에 따른 홍삼 융합청국장 가수분해물의 특성은 protease(KMF-G)가 가수분해도, 칼슘결합능, 총 페놀화합물 함량이 가장 높은 것으로 나타났으며, 혈전용해능과 ACE 저해활성 또한 87.10 unit, 67.17%로 가장 높게 나타났다. Protease(KMF-G) 농도에 따른 영향을 조사한 결과 0.02%에서 적합하였다. 홍삼 융합청국장의 가수분해시간에 따른 영향은 60분과 90분에서 가장 양호한 결과를 나타내었으나 유의적 차이가 나타나지 않았으며, 홍삼 융합청국장 가수분해물의 불쾌취와 쓴맛은 60분 이상에서 개선되었으므로 최적 가수분해시간은 60분으로 설정할 수 있었다. 홍삼 융합청국장의 총 진세노사이드 함량은 9.197 mg/g이고, 홍삼 융합청국장 가수분해물의 함량은 11.707 mg/g이었다. 이상의 결과 protease(KMF-G), 0.02%를 첨가하여 60분간 가수분해 하였을 때 이화학적 특성, 혈전용해능 및 ACE 저해활성이 향상된 홍삼 융합청국장 가수분해 조건을 설정할 수 있었다.

Keywords

References

  1. Choi KJ. 1991. Component and quality control of material ginseng. Kor J Pharmacol 15: 247-256
  2. Kim NM, Lee JS, Lee BH. 1999. Effects of $\beta$-amylase and transglucosidase on the qualities of red ginseng extract. J Ginseng Res 23: 93-98
  3. Kim JS, Yoo SM, Choe JS, Park HJ, Hong SP, Chang CM. 1998. Physicochemical properties of traditional cheonggukjang produced in different regions. J Korean Soc Appl Biol Chem 41: 377-383
  4. Woo SM, Kim KS, Kwon JH, Jeong YJ. 2005. Selection and fermentation characteristics of cheonggukjang strains. Korean J Food Preserv 13: 77-82
  5. Choi UK, Son DH, Ji WD, Im MH, Choi JD, Chung YG. 1998. Changes of taste components and palatability during cheonggukjang fermentation by Bacillus subtilis DC-2. J Korean Soc Food Sci Nutr 27: 840-845
  6. Seok YR, Kim YH, Kim S, Woo HS, Kim TW, Lee SH, Choi C. 1994. Change of protein and amino acid composition during cheonggukjang fermentation using Bacillus licheniformis CN-115. J Korean Soc Appl Biol Chem 37: 65-71
  7. Joo HK. 1996. Studies on chemical composition of commercial chunggukjang and flavor compounds of cheonggukjang by mugwort (Artmisia asiatica) or red pepper seed oil. Korea Soybean Digest 13: 44-56
  8. Choi UK, Lee SI, Son DH, Ji WD. 2002. Changes of flavor during cheonggukjang fermentation by Bacillus sp. CS-17. J Korean Soc Hygienic Sciences 8: 167-173
  9. Choi SH, Ji YA. 1989. Changes in flavor of cheonggukjang during fermentation. Korean J Food Sci Technol 21: 229-234
  10. Lee YL, Kim SH, Choung NH, Yim MH. 1992. A study on the production of viscous substance during the cheonggukjang fermentation. J Korean Agric Chem Soc 35: 202-209
  11. Kim YT, Kim WK, Oh HI. 1995. Screening and identification of the fibrinolytic bacterial strain from cheonggukjang. Kor J Appl Microbiol Biotechnol 23: 1-5
  12. Jung YK, Lee YK, No HK, Kim SD. 2006. Effect of chitosan on quality characteristics of cheonggukjang. J Korean Soc Food Sci Nutr 35: 476-481 https://doi.org/10.3746/jkfn.2006.35.4.476
  13. In JP, Lee SK. 2004. Effect of Yucca (Yucca shidigera) extract on quality characteristics of cheonggukjang using Bacillus subtilis p01. J Korean Soc Appl Biol Chem 47: 176-181
  14. Jung YK, Lee YK, No HK, Kim SD. 2006. Effect of sea tanble on fermentation and quality characteristics of cheonggukjang. Korean J Food Preserv 13: 95-101
  15. Shon MY, Kim MH, Park SK, Park JR, Sung NJ. 2002. Taste components and palatability of black bean cheonggukjang added with kiwi and radish. J Korean Soc Food Sci Nutr 31: 39-44 https://doi.org/10.3746/jkfn.2002.31.1.039
  16. Jeong YJ, Woo SM, Kwon JH, Choi MS, Sung JH, Lee JW. 2007. Quality characteristics of red ginseng fusion cheonggukjang by the addition methods of red ginseng. J Korean Soc Food Sci Nutr 36: 889-895 https://doi.org/10.3746/jkfn.2007.36.7.889
  17. KMHW. 1997. Korean Food Standard Code. The Korean Ministry of Health and Welfare. p 507-510
  18. An TH. 1998. Effects of enzyme modification and soy protein isolate on the quality of cheese analogs containing lipoxygenase-defected soy milk. PhD Dissertation. Chung Ang University, Seoul. Korea
  19. Lowry OH, Rosebrough NJ, Farr L, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275
  20. AOAC. 1980. Official Methods of Analysis. 13th ed. Association of Official Analytical Chemists, Washington, DC. p 176-180
  21. Pyun JW, Hwang IK. 1996. Preparation of calcium-fortified soymilk and in vitro digestion properties of its protein and calcium. Korean J Food Sci Technol 28: 995-1000
  22. Kim HK, Kim GT, Kim DK, Choi WA, Park SH, Jeong YK. 1997. Purification and characterization of a novel fibrinolytic enzyme from Bacillus sp. KA38 originated from fermented fish. J Fermentation and Bioengineering 84: 307-312 https://doi.org/10.1016/S0922-338X(97)89249-5
  23. Chshman DW, Cheung HS. 1971. Spectrometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem Pharmacol 20: 1637-1648 https://doi.org/10.1016/0006-2952(71)90292-9
  24. Kim SY, Park PSW, Rhee KC. 1990. Functional properties of proteolytic enzyme modified soy protein isolate. J Agric Food Chem 38: 651-664 https://doi.org/10.1021/jf00093a014
  25. Cho YJ, Cha WS, Bok SK, Kim MU, Chun SS, Choi UK. 2000. Production and separation of anti-hypertensive peptide during cheonggukjang fermentation with Bacillus subtilis CH-1023. J Korean Soc Agric Chem Biotechnol 43: 247-252
  26. 식품의약품안정청. 2003. 건강기능식품의 기준 및 규격제정(안) 입안예고. 공고 제2003-62호. p 36-39
  27. Choe JS, Yoo SM, Kim HI, Kim JS, Chang CM. 1999. Volatile compounds of cheonggukjang prepared by different fermentation methods and soybean cultivars. J Korean Soc Agric Chem Biotechnol 42: 111-115
  28. Allagheny N, Obanu ZA, Campbell-Platt G, Owens JD. 1996. Control of ammonia formation during Bacillus subtilis fermentation of legumes. Int J Food Microbiol 29: 321-333 https://doi.org/10.1016/0168-1605(95)00069-0
  29. Larroche C, Besson I, Gros JB. 1999. High pyrazine production by Bacillus subtilis in solid substrate fermentation on ground soybeans. Process Biochem 34: 667-674 https://doi.org/10.1016/S0032-9592(98)00141-1

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