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Optimization of Extraction Conditions for Functional Components of Roasted Pleurotus eryngii by Microwave-Assisted Extraction

볶음 새송이버섯 기능성분의 마이크로웨이브 추출조건 최적화

  • 이명희 (경북과학대학 바이오식품과) ;
  • 윤성란 (경북과학대학 전통식품연구소) ;
  • 조덕조 (경북과학대학 전통식품연구소) ;
  • 김현구 (한국식품연구원) ;
  • 이기동 (대구신기술사업단 바이오산업지원센터)
  • Published : 2007.08.30

Abstract

Response surface methodology was employed to optimize extraction conditions for finding the maximizing the functional properties of roasted Pleurotus eryngii. Based on the central composite design, independent variables were ethanol concentration ($0{\sim}100%$), extraction time ($1{\sim}9$ min) and microwave power ($25{\sim}125$ W). Soluble solid content, electron donating ability and nitrite-scavenging ability were mainly affected by ethanol concentration, but ACE inhibition activity was largely affected by extraction time. The optimum ranges of extraction conditions resulting from superimposing the response surface were predicted to be ethanol concentration ($25{\sim}50%$), extraction time ($3{\sim}9$ min) and microwave power ($80{\sim}125$ W). Total protein and total phenolic compound content of optimal extracts were 45.80 mg/g and 7.42 mg/g, respectively. In phenolic compounds of roasted Pleurotus eryngii extracts, protocatechuic acid was the highest concentration at 1226.32 ${\mu}g/g$, followed by salicylic acid, catechin, p-hydroxybenzoic acid, caffeic acid, coumaric acid and hesperidin.

볶음 새송이버섯의 기능성이 우수한 최적 마이크로웨이브 추출조건을 설정하기 위하여 에탄올 농도($0{\sim}100%$), 추출시간($1{\sim}9$ min) 및 마이크로웨이브 파워($25{\sim}125$ W)에 따른 중심합성실험계획으로 반응표면분석하였다. 그 결과 가용성 고형분 함량, 전자공여능 및 아질산염 소거능의 경우 에탄올 농도에 영향을 많이 받는 것으로 나타났으며, ACE 저해활성은 추출시간에 영향을 많이 받는 것으로 나타났다. 볶음 새송이버섯의 최적 추출조건은 에탄올 농도 $25{\sim}50%$, 추출시간 $3{\sim}9$ min, 마이크로웨이브 파워 $80{\sim}125$ W일 때로 나타났다. 최적 추출물의 총 단백질 함량은 45.80 mg/g, 총 페놀성 화합물 함량은 7.42 mg/g으로 나타났으며, 페놀성 물질 중 protocatechuic acid가 1226.32 ${\mu}g/g$으로 가장 많았고, 다음으로 salicylic acid, catechin, p-hydroxybenzoic acid, caffeic acid, coumaric acid 및 hesperidin 순인 것으로 나타났다.

Keywords

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