Effect of Far-infrared Irradiation on the Antioxidant Activity of Extracts from Phellinus igniarius and Ganoderma lucidum

원적외선 처리가 상황버섯과 영지버섯 추출물의 항산화능에 미치는 영향

  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Ju, Young-Cheoul (Mushroom research Institute, Gyeonggido Agricultural Research and Extension Service) ;
  • Kim, Jeong-Han (Mushroom research Institute, Gyeonggido Agricultural Research and Extension Service)
  • 이승철 (경남대학교 식품생명학과) ;
  • 주영철 (경기도농업기술원 버섯연구소) ;
  • 김정한 (경기도농업기술원 버섯연구소)
  • Published : 2007.08.31

Abstract

The antioxidant activities of Phellinus igniarius (PI) and Ganoderma lucidum (GL) extracts were evaluated after far-infrared (FIR) irradiation. PI and GL were irradiated at $120^{\circ}C$ for 30, 60, 90 and 120 min with a FIR heater (2-14 ${\mu}m$), and then extracted by distilled water for 1 hr. Total sugar contents (TSC), total phenolic contents (TPC), radical scavenging ability (RSA), and reducing power (RP) were significantly increased by FIR irradiation. When PI was irradiated for 120 min, the TSC, TPC, RSA, and RP values of the PI extracts increased from 10.1 to 25.9 mg/g, 6.1 to 13.4 mg/g, 23.8 to 51.5%, and 0.222 to 0.363, respectively, compared to the untreated controls. The GL extracts, under the same conditions ($120^{\circ}C$ for 120 min), also increased in TSC, TPC, RSA, and RP from 11.1 to 22.6 mg/g, 2.5 to 5.8 mg/g, 14.5 to 18.6%, and 0.271 to 0.296, respectively. These results indicate that appropriate FIR irradiation of PI and GL could enhance the antioxidant activities of their extracts by increasing amounts of phenolic and sugar compounds.

원적외선 조사가 상황버섯과 영지버섯의 항산화능에 미치는 영향을 조사하기 위하여 30, 60, 90 그리고 120분 간격으로 원적외선을 조사를 한 후, 물 추출물을 제조하여 실험에 이용하였다. 상황버섯 추출물은 원적외선 처리시간에 비례하여 갈변도, 총당, 총페놀, 라디컬소거능 및 환원력이 증가하였으며, 120분 처리 후 갈변도는 2.1 배, 총당은 2.6배, 총페놀은 2.2배 , 라디컬 소거능은 2.2배, 환원력은 1.6 배 증가하였다. 또한, 원적외선 120분 조사에 의해 영지버섯 추출물의 갈변도, 총당, 총페놀 및 라디컬소거능도 각각 1.7배, 2.0배, 2.3배, 1.3배 증가하였다. 그러나 환원력은 유의적인 차이를 보이지 않았으며, 전체적인 효과는 상황버섯에 비해 다소 낮았다. 이상의 결과 원적외선 조사가 상황버섯과 영지 버섯의 당류 및 페놀화합물의 추출을 촉진하여 항산화능을 향상시킴을 확인하였다.

Keywords

References

  1. Choi JH, Ha TH, Rho YD. Studies on the main factors affecting the mycelial growth of Phellinus linteus. Korean J. Mycol. 24: 214-222 (1996)
  2. Ikekawa J, Nakamishi M, Uehara N, Chihara G, Fukuoka F. Antitumor action of some Basidiomycetes especially Phellinus linteus. Gann 59: 155-157 (1968)
  3. Shiao MS. Natural products of the medicinal fungus Ganoderma lucidum: occurrence, biological activities, and pharmacological functions. Chem. Rec. 3: 172-180 (2003) https://doi.org/10.1002/tcr.10058
  4. Wasser SP, Weiss AL. Therapeutic effects of substances occurring in higher Basiodiomycetes mushroom: a modern perspective. Crit. Rev. Immunol. 19: 65-69 (1999)
  5. Yen GC, Wu JY. Antioxidant and radical scavenging properties of extracts from Ganoderm tsugae. Food Chem. 65: 375-379 (1999) https://doi.org/10.1016/S0308-8146(98)00239-8
  6. Niwa Y, Miyachi Y. Antioxidant action of natural health products and Chinese herbs. Inflammation 10: 79-91 (1986) https://doi.org/10.1007/BF00916043
  7. Lee SC, Kim JH, Jeong SM, Kim DR, Ha JU, Nam KC, Ahn DU. Effect of far-infrared radiation on the antioxidant activity of rice hull. J. Agr. Food Chem. 51: 4400-4403 (2003) https://doi.org/10.1021/jf0300285
  8. Kim SY, Jeong SM, Jo SC, Lee SC. Application of far-infrared irradiation in the manufacturing process of green tea. J. Agr. Food Chem. 54: 9943-9947 (2006) https://doi.org/10.1021/jf062524+
  9. Lee SC, Jeong SM, Kim SY, Nam KC, Ahn DU. Effect of farinfrared radiation on the antioxidant activity of defatted sesame meal extract. J. Agr. Food Chem. 53: 1495-1498 (2005) https://doi.org/10.1021/jf048620x
  10. Dubolis M, Gillers KA, Hamilton JK, Rebers PA. Smith J. Colorimeteric method for determination of sugar and related substance. Anal. Chem. 28: 350-352 (1956) https://doi.org/10.1021/ac60111a017
  11. Gutfinger T. Polyphenols in olive oils. J. Am. Oil Chem. Soc. 58: 966-968 (1981) https://doi.org/10.1007/BF02659771
  12. Blois MS. Antioxidant by the use of a stable free radical. Nature 181: 1199-1200 (1958) https://doi.org/10.1038/1811199a0
  13. Oyaizu M. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44: 307-315 (1986) https://doi.org/10.5264/eiyogakuzashi.44.307
  14. Lee HS, Son JY. Antioxidant and synergist effect of extract isolated from commercial green, oolong, and black tea. Korean J. Food Nutr. 15: 377-381 (2002)
  15. Song HN, Oh SW. Optimization of extraction and clarification condition for preparation of liquid extract tea from artificially cultivated Phellinus linteus. J. Korean Soc. Food Sci. Nutr. 31: 636- 641 (2002) https://doi.org/10.3746/jkfn.2002.31.4.636
  16. Shahidi F, Wanasundara PK. Phenolic antioxidant. Crit. Rev. Food Sci. 32: 67-103 (1992) https://doi.org/10.1080/10408399209527581
  17. Lo KM, Cheung PCK. Antioxidant activity of extracts from the fruiting bodies of Agrocybe aegerita var. alba. Food Chem. 89: 533-539 (2005) https://doi.org/10.1016/j.foodchem.2004.03.006
  18. Mau JL, Lin HC, Song SF. Antioxidant properties of several specialty mushrooms. Food Res. Int. 35: 519-526 (2002) https://doi.org/10.1016/S0963-9969(01)00150-8
  19. Wada T, Hayashi Y, Shibata H. Asiaticusin A and B, novel prenylated phenolics from Boletinus asiaticus and B. paluster (Boletaceae) fungi. Biosci. Biotech. Bioch. 60: 120-121 (1996) https://doi.org/10.1271/bbb.60.120
  20. Ribeiro B, Rangel J, Valentao P, Baptista P, Seabra RM, Andrade PB. Contents of carboxylic acids and two phenolics and antioxidant activity of dried portuguese wild edible mushrooms. J. Agr. Food Chem. 54: 8530-8537 (2006) https://doi.org/10.1021/jf061890q
  21. Chung HS, Youn KS. Comparison of pretreatment methods for extraction of selected components from Ganodrerma lucidum. Korean J. Food Pres. 12: 130-134 (2005)
  22. Chung HS, Youn KS. Effects of microwave, ultrasound and roasting pretreatment on hot water extraction of Acanthopanax senticosus. Korean J. Food Pres. 12: 146-150 (2005)
  23. Inoue S, Kabaya M. Biological activities caused by far-infrared radiation. Int. J. Biometeorol. 33: 145-150 (1989) https://doi.org/10.1007/BF01084598
  24. Choi Y, Lee SM, Chun J, Lee HB, Lee J. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of shitake (Lentinus edodes) mushroom. Food Chem. 99: 381-387 (2006) https://doi.org/10.1016/j.foodchem.2005.08.004
  25. Kim HK, Choi YJ, Jeong SW, Kim KH. Functional activities of microwave-assist extracts from Lyophyllum ulmarium. Korean J. Food Pres. 9: 385-390 (2002)
  26. Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autooxidation of soybean oil in cyclodextrin emusion. J. Agr. Food Chem. 40: 945-948 (1992) https://doi.org/10.1021/jf00018a005