Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidative Activity of Gynostemma pentaphyllum Makino Extracts

덩굴차(Gynostemma pentaphyllum Makino) 추출물의 항산화 효과

  • Published : 2007.08.31
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Abstract

This study was carried out to examine the antioxidant activity of Gynostemma pentaphyllum Makino. Using the DPPH method, we found that free radical scavenging activity was strong in all the fractions except the water fraction of the water extract (GPW) and ethanol extract (GPE) of G pentaphyllum Makino. Pseudo-SOD activity was highest in the diethyl ether fraction of the ethanol extract, while the other fractions of the ethanol and water extracts were lower. For xanthine oxidase inlribition activity, the diethyl ether fraction and butanol fraction of GPW, and the diethyl ether fraction and butanol fraction of GPE, showed activities over 80%. Nitrite scavenging ability was strong (over 60%) in all the GPW and GPE fractions. The diethyl ether fraction and butanol fraction of GPW had more effective nitrite scavenging abilities than the other extract fractions. These results suggest that the extracts of G pentaphyllum Makino can be used as a functional material in a tea or drink.

덩굴차(Gynostemma pentaphyllum Makino)의 물(GPW)과 에탄올 추출물(GPE)의 각 용매 획분에 대한 항산화효과를 검토한 결과는 다음과 같다. 전자공여능은 GPW과 GPE의 water획분을 제외한 나머지 모든 획분에서 모두 90%이상의 강한 활성을 나타냈으며, SOD유사 활성능은 GPW의 water획분 40%, GPE의 diethyl ether 획분 95%의 활성만이 나타났다. Xanthine oxidase 저해활성은 GPW의 ethyl ether획분, butanol획분, GPE의 diethyl ether획분, buthanol획분에서 80%이상으로 높았으며, 아질산염 소거능은 GPW의 diethyl ether획분과 buthanol획분에서 95%이상, GPE의 diethyl ether획분, chlorofonn획분, butanol획분이 80%이상으로 높은 것으로 나타났다. 이와같이 덩굴차 추출물은 항산화 관련 free radical 소거(electron donating ability, superoxide dismutase acitivity, nitrite scavenging activity)계 및 생성 억제(xanthine oxidase inhibition activity)계에 있어서 분획물에 따라 다소 차이는 있었으나 전자공여능과 xanthine oxidase 저해 활성은 90%이상의 전반적으로 강한 활성이 있었으며 아질산염 소거능 또한 비극성 및 극성 분획물에서 전반적으로 소거활성이 나타났다. 덩굴차 추출물은 소거 계뿐만 아니라 생성 억제계에서도 전반적으로 항산화 활성을 보인 것으로 보아 이것은 덩굴차가 다류로서뿐만 아니라 기능성 식품의 부재료로서의 가능성이 충분히 있는 것으로 판단된다.

Keywords

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