Anti-glycation Activities from Various Agricultural Products

단백질 glycation 저해효과가 있는 식품소재

  • Published : 2007.08.31


The ethanolic extracts of 83 kinds of agricultural products, including cereals, vegetables, and Chinese herbs, were tested for their inhibitory activities on protein cross-linking using the $[^{14}C]$-N-formyl-lysine incorporation method. Most of the extracts inhibited, but some extracts accelerated, the cross-linking of protein. Of those items with relatively high activities, we selected 20 samples to test for activity against AGE fonnation using the fluorophotometric method. The ethanol extract of buckwheat that was genninated for 1 day (GB-01) was detennined to have the highest activity with both methods. The ethanol extract of GB-01 was further fractionated by organic solvents, including chloroform, ethyl acetate, butanol, and water, in order of increasing polarity. The fraction that was extracted with ethyl acetate presented the highest protein glycation inhibitory activity (95.2% inhibition at the 100 ug/mL addition level). Polyphenol content analysis by HPLC showed that the amounts of rutin and quercetin were increased with the separation procedures. Finally, there was a significant relationship between activity and polyphenol content in the partially purified samples (p<0.05).

식품소재의 단백질 glycation 저해효과를 조사하기 위하여 곡류, 채소류 및 생약재 등 83종 에탄올 추출물에 대한 단백질 가교결합 저해효과를 $[^{14}C]$-N-formyl-lysine incorporation method를 이용하여 측정하였다. 대부분의 추출물에서 저해효과가 나타났으나 일부 추출물은 단백질의 가교를 촉진하는 것으로 나타났다. 상대적으로 높은 활성을 나타낸 시료 중 20개를 선별하여 형광광도법을 이용한 AGE형성 억제능을 조사한 결과, 1일 발아한 메밀의 추출물 (GB-l)이 가장 높은 저해활성을 가지는 것으로 나타났다. 차후 GB-0l은 콜로로포름, 에틸아세테이트, 부탄올 및 물로 분획되었으며 그 중 에틸아세테이트 분획이 가장 높은 단백질 glycation 저해활성(95.2% inhibitory activity at 100 ug/mL addi- tion level)을 보였다. HPLC를 이용한 폴리페놀 분석결과 발아 및 추출, 분획과정을 거친 메밀의 아세테이트 분획은 rutin 및 quercetin 함량이 상당히 증가한 것으로 나타나서 부분정제물의 rutin 및 quercetin 함량과 저해활성사이에 높은 상관성을 나타내었다.



  1. Horiuchi S, Araki N, Moino Y. Immunochemical approach to characterize advanced glycation end products of the Maillard reaction. J. Biol. Chem. 266: 7329-7332 (1991)
  2. Brownlee M, Advanced glycation end products in diabetic complications. Curr. Opin. Endocrinol. Diabetes 3: 291-297 (1996)
  3. Cohen MP, Urdaniva E, Surma M, Wu VY. Increased glycosylation of glomerular basement membrane collagen in diabetes. Biochem. Bioph. Res. Commun. 95: 765-769 (1980)
  4. Bierhaus A, Hofmann MA, Ziegler R, Nawroth P. AGEs and their interaction with AGE-receptors in vascular disease and diabetes mellitus: I. The AGE concept. Cardiovasc. Res. 37: 586- 600 (1998)
  5. Song JY, Ihm SH, Suh JY, Cho YJ, Yoo HJ, Paril SW, Ihm JH. Effect of advanced glycation end products on rat aortic vascular smooth muscle cells. Korean J. Diabetes 26: 91-99 (2002)
  6. Taguchi T, Dugiura M, Humada Y, Miwa I. Inhibition of advanced protein glycation by a Schiff base between amin guanidine and pyridoxal. Eur. J. Pharmacol. 378: 283-289 (1999)
  7. Soules-Liparota T, Cooper M, Papazoglou D. Retardation by aminoguanidine of development of albuminuria, mesangial expansion, and tissue fluorescence in streptozotocin-induced diabetic rats. Diabetes 40: 1328-1335 (1991)
  8. Ellis EN, Good BH. Prevention of glomerular basement membrane thickening by aminoguanidine in experimental diabetes mellitus. Metabolism 40: 1016-1019 (1991)
  9. Cameron NE, Cotter MA, Dines K, Love A. Effect of aminoguanidine on peripheral nerve function an polyol pathway metabolites in streptozotocin-diabetic rats. Diabetologia 35: 946- 950 (1992)
  10. Ou P, Wolff SP. Aminoguanidine: a drug proposed for the prophylaxis in diabetes inhibits catalase and generates hydrogen peroxide in vitro. Biochem. Pharmacol. 43: 1139-1144 (1993)
  11. Lee KW. Method for the preparation of N-formyl lysine and the analysis of glycation induced-protein crosslinking assay thereby. KR patent No. 0377336 (2002)
  12. Yamaguchi F, Ariga T, Yoshimura Y, Nakazawa H. Antioxidative and anti-glycation activity of garcinol from Garcinia indica fruit rind. J. Agr. Food Chem. 48: 180-185 (2000)
  13. Ohara J, Ohinata H, Muramatsu N, Matsuhashi T, Oike T. Determination of rutin in buckwheat foods by high performance liquid chromatography. Nippon Shakuhin Kogyo Gak. 36: 114-119 (1989)
  14. Mukoda T, Sun B, Ishiguro A. Antioxidant activities of buckwheat hull extract toward various oxidative stress in vitro and in vivo. Biol. Pharm. Bull. 24: 209-213 (2001)
  15. Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, Cazin M, Cazin JC, Bailleul F, Trotin F. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J. Ethnopharm. 72: 35-42 (2000)
  16. Morimitsu Y, Yoshida K, Esaki S, Hirota A. Protein glycation inhibitors from thyme (Thymus Vulgaris). Biosci. Biotech. Biochem. 59: 2018-2021 (1995)
  17. Ford SM, Hook JB, Bond JT. The effects of butylated hydroxyanisole and butylated hydroxytoluene on renal function in the rat. II. Effects on organic acid and base transport. Food Cosmet. Toxicol. 18: 21-26 (1980)
  18. Frankel EN, Huang SW, Aeschbach R, Prior E. Antioxidant activity of a rosemary extract and its constituents, carnosic acid, carnosol, and rosmarinic acid, in bulk oil and oil-in-water emulsion. J. Agr. Food Chem. 44: 131-135 (1996)
  19. Marshall HG, Pomeranz Y, Chapter G. In: Buckwheat description, breeding, production, and utilization. Volume V. p. 157. Advances in Cereal and Technology, American Association of Cereal Chemists, St. Paul, MN, USA (1982)
  20. Li SQ, Zhang QH. Advances in the development of functional foods from buckwheat. Cri. Rev. Food Sci. 41: 451-464 (2001)