Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Hepatoprotective Effect of Fermented Artemisia princeps PAMPANINI by Lactic Acid Bacteria

발효강화쑥의 간장해 보호효과

  • Choi, Hyuck-Jae (East-West Medical Research Institute, Kyung Hee University) ;
  • Kim, Eun-Jin (East-West Medical Research Institute, Kyung Hee University) ;
  • Han, Myung-Joo (Department of Food and Nutrition, Kyung Hee University) ;
  • Baek, Nam-In (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Kim, Dong-Hyun (College of Pharmacy, Kyung Hee University) ;
  • Jung, Hae-Gon (Ganghwa Argicultural R&D Center) ;
  • Kim, Nam-Jae (East-West Medical Research Institute, Kyung Hee University)
  • 최혁재 (경희대학교 동서의학연구소) ;
  • 김은진 (경희대학교 동서의학연구소) ;
  • 한명주 (경희대학교 식품영양학과) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사센타) ;
  • 김동현 (경희대학교 약학대학) ;
  • 정해곤 (강화농업기술센타) ;
  • 김남재 (경희대학교 동서의학연구소)
  • Published : 2007.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Artemisia princeps PAMPANINI has been used in traditional medicine of the treatment of inflammatory, liver dysfunction and order disorder in the far east countries including Korea. The present study was carried out to investigate the hepatoprotective effects of ethanol extract of Artemisia princeps (AP) and its fermented agents (AP-F) by lactic acid bacteria derived from human intestinal bacteria on liver injured rat induced by $CCl_4$ and d-galactosamine. Hepatoprotective activity was monitored by estimating serum ALT, AST, ALP, LDH, superoxide dismutase (SOD), glutathione redeuctase (GR) and glutathione peroxidase (Gpx) activities in the liver injured by hepatotoxin. Pretreating rats with AP or AP-F at the same dosage regimen significantly suppressed the acute elevation of serum transaminase, ALP, LDH and GR activities, and significantly increased the lowering of blood SOD and GR activites induced by hepatoxin. Based on these findings, it is presumed that AP and APF may have the hepatoprotective effect on $CCl_4$ and d-galactosamine-induced hepatotoxicity rat.

Keywords

References

  1. Lee, S. J. (1975) Studies on the identification of Korean traditional folk medicine(I). Korean J. Raw Med., 6: 75-80
  2. 이창복 (1982) 대한식물도감, 761. 향문사, 서울
  3. 김두진 (2002) 한국 쑥문화 연구, 한양대학교 대학원 석사학위논문
  4. 이태호 편저 (1977) 新訂對譯 方藥合編. 147. 행림출판사, 서울
  5. 식품의약품안전청 (2006) 대한약전외 한약(생약)규격집, 248. 도서출판 동원문화사, 서울
  6. 高木敬次郞, 木村正康, 原田正敏, 大塚恭男 (1980) 和漢藥 物學, 165. 南山堂, 東京
  7. Cho Y. H. and Chiang M. H. (2001) Essential oil composition and antibacterial activity of Artemisia capillaris, Artemisia argyi, and Artemisia princeps. Kor. J. Intl. Agri., 13: 313-320
  8. Ryu S. N., Kamg S. S., Kim J. S. and Ku B. I. (2004) Quantitative analysis of eupatilin and jaceosidin in Artemisia herba. Korean J. Crop. Sci., 49: 452-456
  9. 방면호, 김동현, 유종수, 이대영, 송명종, 양혜정, 정태숙, 이경태, 최명숙, 정해곤, 백남인 (2005) 식용식물자원으로부터 활성물질의 탐색-XIV. 사자발쑥(Artemisia herba)의 전초로부터 flavonoid 화합물의 분리. J. Korean Soc. Appl. Biol. Chem., 48: 418-420
  10. 전국한의과대학 본초학교수 공편저 (1991) 본초학, 405. 도서출판 영림사, 서울
  11. Lee E. B. (1995) The effect of Artemisia Herba on gastric lesion and ulcers in rats with isolation of eupatilin, in Unesco Regional Seminar on the Chemistry, Pharmacology and Clinical use of flavonoid compounds. Oct. 11-15. Chungnam National University. Taejon, Korea. 13-20
  12. 오태영, 류병권, 박정배, 이상득, 김원배, 양중익, 이은방 (1996) 애엽추출물, DA-9601의 실험적 위궤양 모델에 대한 항궤양 효과 및 기전연구. 응용약물학회지., 4: 111-121
  13. Oh T. Y., Ryu B. K., Yang J. I., Kim Y. B., Park J. B., Lee S. D. and Lee E. B. (1996) Studies on antiulcer effects of DA-9601, an Artemisia Herba extract, against experimental gastric ulcer and its mechanism. J. Appl. Pharmacol., 4: 111- 121
  14. Oh T. Y., Ryu B. K., Ko J. I., Ahan B. Y., Kim S. H., Kim W. B., Lee E. B., Jin J. H. and Hahm K. B. (1997) Beneficial effect of DA-9601, an Artemisia Herba extract, against naproxen-induced gastric damage in arthritic rats. Arch. Pharm Res., 20: 414-419 https://doi.org/10.1007/BF02973932
  15. Oh T. Y., Ahn G. J., Choi S. M., Ahn B. O. and Kim W. B. (2005) Increased susceptiibility of ethanol-treated gastric mucosa to naproxen and its inhibition by DA-9601, and Artemisia asiatica extract. World J. Gastroenterol., 47: 7450- 7456
  16. Twaij H. A. and Badr A. A. (1988) Hypoglycemic activity of Artemisia gerba alba. J. Ethnopharmacol., 24: 123-126 https://doi.org/10.1016/0378-8741(88)90143-2
  17. 김미향 (2006) 쑥이 갱년기 장애 유도 흰쥐의 혈중 지질 및 결합조직 중 Collagen 함량에 미치는 영향. 생약학회지., 37: 324-330
  18. Koshihara, Y., Neichi, T., Murota, S., Lao, A., Fujimoto Y. and Tatsuno T. (1983) Selective inhibition of 5-lipogenase by natural compound isolated from Chinese plants, Artemisia rubripes Nakai. FEBS Lett., 158: 41-44 https://doi.org/10.1016/0014-5793(83)80672-3
  19. Seo H. J. and Surh Y. J. (2001) Eupatilin, a pharmacologically active flavone derived from Artemisia plants, induce apoptosis in human promyelocytic leukemia cells. Mutat Res., 496: 191-198 https://doi.org/10.1016/S1383-5718(01)00234-0
  20. Heo H. J., Cho H. Y., Hong B., Kim W. B., Kim E. K., Kim B. G. and Shin D. H. (2001) Protective effect of 4',5-dihy-droxy-3',6,7-trimethoxy flavone from Artemisia asiatica against AB-oxidative stress in PC12 cells. Amyloid., 8: 194-201 https://doi.org/10.3109/13506120109007362
  21. Klayman, D. L. (1985) Qinghaosu(artemisinin): an antimalarial drug from China. Science., 228: 1049-1055 https://doi.org/10.1126/science.3887571
  22. Luo, X. D. and Shen, C. C. (1987) The chemistry, pharmacology and clinical applications of Qinghaosu(artemisinin) and its derivatives. Med. Res. Rev., 7: 29-52 https://doi.org/10.1002/med.2610070103
  23. 류수노, 한상숙, 김관수, 정해곤 (2006) 수집약쑥의 유효성분 함량변이. 한국작물학회지., 51(S): 220-223
  24. Lee, S. H., Shin Y. W., Bae E. H., Lee B., Min S. W., Baek N. I., Chung H. G., Kim N. J. and Kim D. H. (2006) Lactic acid bacteria increase antiallergic effect of Artemisia princeps Pampanini SS-1. Arch. Pharm. Res., 29: 752-756 https://doi.org/10.1007/BF02974075
  25. Lin L. T., Liu L. T., Cjiang L. C. and Lin C. C. (2002) In vitro Anti-hepatoma activity of fifteen natural medicines from Canada. Phytother. Res., 16: 440-444 https://doi.org/10.1002/ptr.937
  26. S. Maeda, K. Sudo, Y. Miyamoto, S. Takeda, M. Shinbo, M. Aburada, Y. Ikeya, H. Taguchi and M. Harada (1982) Pharmacological studies on Schzandra Fruits. II. Yakugau Zasshi., 102: 579-588 https://doi.org/10.1248/yakushi1947.102.6_579
  27. Vimal, V. and Devaki, T. (2004) Hepatoprotective effect of allicin on tissue defense system in galactosamine/endotoxin challenged rats, J. Ethnopharmacol., 90: 151-154 https://doi.org/10.1016/j.jep.2003.09.027
  28. Lin, C. C., Shieh, D. E. and Yen, M. H.(1997) Hepatoprotective effect of the fractions of Ban-zhi-lian on experimental liver injuries in rats. J. Ethnopharmacol., 56: 193- 200 https://doi.org/10.1016/S0378-8741(97)00026-3
  29. Reitman, S. and Frankel, S. (1957) A colorimetric method for the determination of serum glutamic oxaloacetic acid and glutamic pyruvic transaminase. Am. J. Clin. Phathol., 28: 56- 63 https://doi.org/10.1093/ajcp/28.1.56
  30. Anh, D. J., Eden, A. and Farley, J. R. (2001) Quantitation of soluble and skeletal alkaline phosphatase, and insoluble alkaline phosphatase anchor-hydrolase activities in human serum. Clinica. Chimica. Acta., 311:137-148 https://doi.org/10.1016/S0009-8981(01)00584-8
  31. Wroblewskin F. and LaDue J. S. (1955) Lactic dehydrogenase activity in blood. Proc. Soc. Exp. Biol. Med., 90: 210- 213
  32. 小澤 光 監修 (1984) 新藥開發のための藥效スクリニング法. 69. 淸至書院, 東京
  33. 阿部博子, 戶田靜男, 大井久代, 久保道德, 有地 滋 (1978) D-galactosamineに代するサイコサポニンの作用. 和漢藥シンポジウム., 11: 7-10
  34. 박은전, 김재백, 손동환, 고건일 (1997) 실험적 간경화 병태모델 비교. 약학회지., 41: 622-628
  35. McCay, B. P., Lai, E. K., Poyer, J. L., DuBose, C. M. and Jansen, E. G. (1984) Oxygen and Carbon-centered free radical formation during carbon tetrachloride metabolism, J. Biol. Chem., 259: 2135-2143
  36. Rechnagel, R. O. and Glende, E. A. Jr. (1973) Carbon tetrachloride hepatotoxicity : an example of lethal cleavage. CRC Crit. Rev. Toxicol., 2: 263-297 https://doi.org/10.3109/10408447309082019
  37. Gravela, E., Albano, E., Dianzani, M., Poli, G. and Slater, T. (1979) Effects of carbon tetrachloride on isolated rat hepatocytes. Biochem. J., 178: 509-512 https://doi.org/10.1042/bj1780509
  38. Hayes (1982) Principles and Method of Toxicology, 407. Gabriel L. Plaa and William R. Hewitt. Raben Press
  39. Terry, D. Oberley, Janice L. Schultz, Ning Li and Larry W. Oberley (1995) Antioxidant enzyme levels as a function of growth state in cell culture. Free Radical Bilo Med., 19: 53- 65 https://doi.org/10.1016/0891-5849(95)00012-M
  40. Shindo, Y. and Hashimoto, T. (1997) Time course of changes in antioxidant enzymes in human skin fibroblasts after UV irradiation. J. Dermatol Sci., 14: 225-232 https://doi.org/10.1016/S0923-1811(96)00578-6
  41. 김안근, 김지현 (2001) 산화적 스트레스 및 항산화제가 항산화효소 활성에 미치는 영향. 응용약물학회지., 9: 249- 257
  42. Zoltn Gregus and Curtis D. Klaassen (1996) Mechanisms of toxicity. In Casarett and Doull's toxicology. 39. Mcgraw-Hill Com. New York
  43. Keppler, D. and Decker, K. (1969) Studies on the mechanism of galactosamine hepatitis ; Accumulation of galactosamine- 1-phosphate and tis inhibition of UDP-glucose pyrophosphorylase. Eur. J. Biochem., 10: 219-225 https://doi.org/10.1111/j.1432-1033.1969.tb00677.x
  44. Decker, K., Keppler, D. and Pausch, J. (1973) The regulation pyridine nucleotide level and its role in experimental hepatitis. Adv. Enzyme Regul., 11: 205-230 https://doi.org/10.1016/0065-2571(73)90017-4
  45. Farber, J. L., Gill, G. and Konishi, Y. (1973) Prevention of galactosamine-induced liver cell necrosis by uridine. Am. J. Pathol., 72: 53-62