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가죽나무(Ailanthus altissima) 물 추출물의 항산화 활성과 Tyrosinase 저해

Antioxidative Activities and Tyrosinase Inhibition of Water Extracts from Ailanthus altissima

  • 이양숙 (대구한의대학교 한방생약자원학과) ;
  • 최진범 (대구한의대학교 한방생약자원학과) ;
  • 주은영 (대구한의대학교 한방생약자원학과) ;
  • 김남우 (대구한의대학교 한방생약자원학과)
  • Lee, Yang-Suk (Dept. of Herbal Biotechnology, Daegu Haany University) ;
  • Choi, Jin-Beom (Dept. of Herbal Biotechnology, Daegu Haany University) ;
  • Joo, Eun-Young (Dept. of Herbal Biotechnology, Daegu Haany University) ;
  • Kim, Nam-Woo (Dept. of Herbal Biotechnology, Daegu Haany University)
  • 발행 : 2007.09.30

초록

한방생명자원으로 사용되고 있는 가죽나무(A. altissima)를 대상으로 뿌리와 줄기, 잎을 $80^{\circ}C$의 조건에서 물을 용매로 추출한 가죽나무 추출물의 전자공여능, 아질산염 소거능, SOD 유사활성 및 XO 저해 활성과 tyrosinase 저해효과를 측정하였다. 전자공여능은 1.0 mg/mL의 농도에서 뿌리(74.83%)>줄기(70.01%)>잎(29.24%)의 순으로 활성을 나타내었다. 아질산염 소거능에서 가죽나무 잎 추출물은 1.0 mg/ mL pH 1.2와 3.0의 조건에서 각각 95%와 89% 이상으로 줄기(55.17%)와 뿌리(33.33%)보다 높은 소거율을 보였으며, 0.5 mg/mL의 농도에서도 각각 93%와 80% 이상의 아질산염 소거효과를 나타내었다. SOD 유사활성능에서도 잎 추출물은 26.77%로 뿌리의 3.82%보다 7배 높았으며, 줄기 추출물에서는 SOD 유사활성 효과가 없었다. XO의 저해율은 세가지 추출물 모두 0.5 mg/mL 이상의 농도에서 약 90% 이상의 저해 활성을 나타내었으며, tyrosinase에 대한 저해 활성은 2.0 mg/mL의 농도에서 잎 추출물이 16.33%으로 뿌리(7.09%)와 줄기(5.21%) 추출물보다 약 2배 높은 저해율을 나타내었다. 이상의 결과로 보아 가죽나무 잎 추출물은 현재 한약재로 사용되고 있는 뿌리와 줄기보다 전자공여능은 낮았으나 뿌리보다 7배 높은 SOD 유사활성 효과와 약 90% 이상의 아질산염 소거능 효과를 나타내었다. 그리고 가죽나무의 잎 추출물은 약 95%의 XO 저해 활성과 약 16%의 tyrosinase 저해효과를 나타내어 뿌리와 줄기보다 저해효과가 높은 것으로 분석되었다. 그러므로 가죽나무의 잎에서도 우수한 생리활성 물질을 다량 함유하는 것으로 생각되며, 뿌리뿐만 아니라 잎도 기능성 식품이나 의약품의 원료 및 첨가물 등 다양한 제품에 이용될 수 있을 것으로 사료된다.

Water extracts from root, stem and leaf of Ailanthus altissima were utilized to determine antioxidant properties such as electron donating ability (EDA), nitrite scavenging ability, superoxide dismutase (SOD)-like activity, and the inhibitory activities of xanthine oxidase (XO) and tyrosinase. The EDA of root extract was the highest as 77.33% at 0.5 mg/mL concentration and that of stem extract was 70.01% at 1.0 mg/mL. The nitrite scavenging ability of leaf extract revealed the highest effect as 95.18% at pH 1.2, 1.0 mg/mL while those of stem and root extracts were 55.17% and 33.33%, respectively. The leaf extract showed the highest SOD like activity as 26.77% at 1.0 mg/mL, the measurement of root extract was 3.82% and that of stem extract was not effective. All kinds of extracts had strong inhibitory activities on XO of over 92% at 1.0 mg/mL. The highest activity on tyrosinase inhibition was obtained from leaf extract of 16.33% at 2.0 mg/mL. The results indicated that among the three extracts, the leaf extract has a strong and extensive antioxidant activity.

키워드

참고문헌

  1. Perry LM. 1990. Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses. The MIT Press, London. p 431
  2. Nguyen MT, Awale S, Tezuka Y, Tran QL, Watanabe H, Kadota S. 2004. Xanthine oxidase inhibitory activity of Vietnamese medicinal plants. Biol Pharm Bull 27: 1414-1421 https://doi.org/10.1248/bpb.27.1414
  3. Fridorich I. 1978. The biological activity of oxygen radicals. Science 201: 875-881 https://doi.org/10.1126/science.210504
  4. Hammond B, Kontos A, Hess ML. 1985. Oxygen radicals in the adult respiratory distress syndrome, in myocardial ischemia and repercussion injury, and in cerebral vascular damage. Can J Physio Pharmacol 63: 173-187 https://doi.org/10.1139/y85-034
  5. Imlay IA, Limm S. 1986. DNA damage and oxygen radical toxicity. Science 240: 1302-1309 https://doi.org/10.1126/science.3287616
  6. Chance B, Sies H, Boveris A. 1979. Hydroperoxide metabolism in mammalian organs. Physiol Rev 59: 527-605 https://doi.org/10.1152/physrev.1979.59.3.527
  7. 구본홍. 1994. 동의보감(허준 저). 대중서관, 서울. p 555, 1456
  8. 國家中医药藥管理局編委會. 1999. 中華本草. 上海科學技術出版社, 上海. Vol 5, p 3-6
  9. 최영전. 1992. 산나물 재배와 이용법. 오성출판사, 서울. p 206
  10. Kim J, Kim HK, Park SW, Choi JW, Lee CK. 1994. Studies on the biological activities of the constituents of Ailanthi cortex radicis II. Acute and renal toxicity of chloroform fraction on epoxide hydrolyzing system in liver. Kor J Pharmacogn 25: 47-50
  11. Kubota K, Fukamiya N, Tokuda H, Nishino H, Tagahara K, Lee KH, Okano M. 1997. Quassinoids as inhibitors of Epstein-Barr virus early antigen activation. Cancer Letters 113: 165-168 https://doi.org/10.1016/S0304-3835(97)04607-7
  12. Pascual-villalobos MJ, Robledo A. 1998. Screening for anti-insect activity in mediterranean plants. Indust Crops Prod 8: 183-194 https://doi.org/10.1016/S0926-6690(98)00002-8
  13. Jeong YM, Park SK, Lee KJ, Kim YM, Yun YG, Kim WS, Han DM, An WG, Yoon YS, Jeon BH. 2003. Effect of Ailanthus altissima on the apoptosis and cell cycle of HL-60 leukemia cell line. Korean J Ori Phys Path 17: 914-922
  14. 江蘚新醫學院. 1979. 中葯大辭典. 1th ed. 下冊, 上海. p 2577- 2579
  15. Kazuya K, Katsuyoshi M, Kazuo K, Taichi O. 1994. Studies on the constituents of Ailanthus integrifolia. Chem Pharm Bull 42: 1669-1671 https://doi.org/10.1248/cpb.42.1669
  16. Barakat HH. 1998. Chemical investigation of the constitutive phenolics of the structure of a new flavone glycoside gallate. Nat Prod Sci 4: 153-157
  17. Lee DG, Chang YS, Park YK, Hahm KS, Woo ER. 2002. Antimicrobial effects of ocotillone isolated from stem bark of Ailanthus altisshima. J Microbiol Biotechnol 12: 854- 857
  18. Kim KW, Baek JK, Jang YW, Kum EJ, Kwon YS, Kim HJ, Sohn HY. 2005. Screening of antibacterial agent against streptococcus mutans from natural and medicinal plants. J Life Science 15: 715-725 https://doi.org/10.5352/JLS.2005.15.5.715
  19. Kim J, Lee CK. 1997. Studies on the biological activities of the constituents of Ailanthi cortex radicis Ⅲ. Antitumor activities of dicholoromethane fraction. Kor J Pharmacogn 28: 54-58
  20. Hwang WG, Lee HC, Kim CK, Chun HJ, Jeung SI, Jeon BH. 2001. Induction of apoptosis in Jurkat T lymphocytes by extract of Ailanthus altissima. Kor J Pharmacogn 32: 274-279
  21. Hwang WG, Lee HC, Kim CK, Kim DG, Lee GO, Yun YG, Jeon BH. 2002. Effect of Ailanthus altissima water extract on cell cycle control genes in Jurkat T lymphocytes. J Pharm Soc Korea 46: 18-23
  22. Lee YS. 2007. Physiological activities of hot water extract from Ailanthus altissima. Korean J Food Preserv 14: 170- 176
  23. Koh JH, Hwang MO, Moon JS, Hwang SY, Son JY. 2005. Antioxidative and antimicrobial activities of pomegranate seed extracts. Korean J Food Cookery Sci 21: 171-179
  24. Sung HS, Kim WJ. 1986. Effect of extracting conditions on the soluble solid's yield of Korean red ginseng. Korean J Food Sci Technol 18: 168-172
  25. Sung HS, Yang CB, Kim WJ. 1985. Effect of extraction temperature and time on saponin composition of red ginseng extract. Korean J Food Sci Technol 17: 265-270
  26. Kim NM, Sung HS, Kim WJ. 1993. Effect of solvents and some extraction conditions on antioxidant activity in cinnamon extracts. Korean J Food Sci Technol 25: 204-209
  27. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200 https://doi.org/10.1038/1811199a0
  28. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric Biol Chem 51: 1333-1338 https://doi.org/10.1271/bbb1961.51.1333
  29. Marklund S, Marklund G. 1975. Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 468-474
  30. Stirpe F, Della Corte E. 1969. The regulation of rat liver xanthine oxidase. J Biol Chem 244: 3855-3861
  31. Yagi A, Kanbara T, Morinobu N. 1987. Inhibition of mushroom-tyrosinase by aloe extract. Planta Med 53: 517-519 https://doi.org/10.1055/s-2006-962798
  32. Lee YS. 2007. Physiological activities of ethanol extracts from different parts of Ailanthus altissima. J Korean Soc Food Sci Nutr 36: 389-394 https://doi.org/10.3746/jkfn.2007.36.4.389
  33. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J Food Sci Technol 36: 333-338
  34. Jang MJ, Woo MH, Kim YH, Jun DY, Rhee SJ. 2005. Effects of antioxidative, DPPH radical scavenging activity and antithrombogenic by the extract of sancho (Zanthoxylum schinifolium). Korean J Nutrition 38: 386-394
  35. Kang MJ, Shin SR, Kim K. 2002. Antioxidative and free radical scavenging activity of water extract from dandelion (Taraxacum officinale). Korean J Food Preserv 9: 253-259
  36. Kim SM, Cho YS, Sung SK. 2001. The antioxidant ability and nitrate scavenging ability of plant extracts. Korean J Food Sci Technol 33: 626-632
  37. Azuma K, Nakayama M, Koshika M, Lppoushi K, Yamaguchi Y, Kohata K, Yamaguchi Y, Ito H, Higashio H. 1999. Phenolic antioxidants from the leaves of Corchorus olitorius L. J Agric Food Chem 47: 3963-3966 https://doi.org/10.1021/jf990347p
  38. Kwon TD, Choi SW, Lee SJ, Chung KW, Lee SC. 2001. Effects of polyphenol or vitamin C ingestion on antioxidative activity during exercise in rats. Kor J Phys Edu 3: 891-899
  39. Lee YS, Joo EY, Kim NW. 2005. Antioxidant activity of extract from the Lespedeza bicolor. Korean J Food Preserv 12: 75-79
  40. Moon SH, Lee MK. 1998. Inhibitory effects of xanthine oxidase by boiled water extract and tannic from persimmon leaves. Korean J Food Nutr 11: 354-357
  41. Yeo SG, Park YB, Kim IS, Kim SB, Park YH. 1995. Inhibition of xanthine oxidase by tea extracts from green tea, oolong tea and black tea. J Korean Soc Food Nutr 24: 154-159
  42. Lee YS, Joo EY, Kim NW. 2006. Polyphenol contents and physiological activity of the Lespedeza bicolor extracts. Korean J Food Preserv 13: 616-622
  43. Jung SW, Lee NK, Kim SJ, Han DW. 1995. Screening of tyrosinase inhibitor from plants. Korean J Food Sci Technol 27: 891-896

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