Effects of Moxi-tar Herbal Acupuncture at LI4 (HapGok) on TNBS-induced Colitis in Rats

합곡의 구진약침이 흰쥐의 염증성 대장염에 미치는 치료 효과

  • Song, Jae-Soo (Dept. of Meridian & Acupoint, Wonkwang University College of Korean Oriental Medicine) ;
  • Yang, Beum-Sik (Dept. of Meridian & Acupoint, Wonkwang University College of Korean Oriental Medicine) ;
  • Kim, Sun-Young (Dept. of Meridian & Acupoint, Wonkwang University College of Korean Oriental Medicine) ;
  • Hong, Jeong-A (Dept. of Meridian & Acupoint, Wonkwang University College of Korean Oriental Medicine) ;
  • Song, Jeong-Bang (Dept. of Meridian & Acupoint, Wonkwang University College of Korean Oriental Medicine) ;
  • Kim, Kyung-Sik (Dept. of Meridian & Acupoint, Wonkwang University College of Korean Oriental Medicine) ;
  • Kim, Jae-Hyo (Dept. of Meridian & Acupoint, Wonkwang University College of Korean Oriental Medicine) ;
  • Kwon, Oh-Sang (Dept. of Meridian & Acupoint, Wonkwang University College of Korean Oriental Medicine) ;
  • Sohn, In-Chul (Dept. of Meridian & Acupoint, Wonkwang University College of Korean Oriental Medicine)
  • 송재수 (원광대학교 한의과대학 경혈학교실) ;
  • 양범식 (원광대학교 한의과대학 경혈학교실) ;
  • 김선영 (원광대학교 한의과대학 경혈학교실) ;
  • 홍정아 (원광대학교 한의과대학 경혈학교실) ;
  • 송정방 (원광대학교 한의과대학 경혈학교실) ;
  • 김경식 (원광대학교 한의과대학 경혈학교실) ;
  • 김재효 (원광대학교 한의과대학 경혈학교실) ;
  • 권오상 (원광대학교 한의과대학 경혈학교실) ;
  • 손인철 (원광대학교 한의과대학 경혈학교실)
  • Published : 2007.06.30

Abstract

Objectives : Ulcerative colitis or Crohn's disease has been recognized as Ha-ri (下痢) or Jang-Byok in Korean oriental medicine. A purpose of the present study is to investigate the anti-inflammatory effect of moxi-tar herbal acupuncture at LI4 (HapGok) on 2,4,6-trinitrobenzene sulphonic acid (TNBS) induced colitis in rats and further elucidate the possibility of herbal acupuncture on ulcerative colitis which is chronic inflammatory disease of the gastrointestinal tract. Methods : Sprague-Dawley rats, weighing $170{\sim}190$ g, were subjected to intrarectal injection of either saline (300 ${\mu}l$, 500 ${\mu}l$) for a control or 2,4,6-trinitrobenzene sulphonic acid (TNBS) (300 ${\mu}l$, 500 ${\mu}l$) for a colitis, Moxi-tar (20 mg/ml) were subcutaneously injected to the LI4 just after the secondary injection time of TNBS in rats. To study the effects of Moxi-tar acupuncture in LI4, body weight, RBC count, WBC count, total protein, Paw edema rate, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels were observed. Results: Moxi-tar acupuncture in LI4 on TNBS-induced colitis inhibited the body weight lose rate but not effect RBC, WBC count. In addition, it inhibited the reduction of total protein concentration, paw edema, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels. Conclusions : It is suggested that moxi-tar herbal acupuncture at LI4 helps to recover TNBS-induced colitis and plays an important role for an treatment of the irritable bowel syndrome (IBS).

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