Environmental Engineering Research

  • 제13권4호
  • /
  • Pages.165-168
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  • 2008
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  • 1226-1025(pISSN)
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  • 2005-968X(eISSN)
대한환경공학회 (Korean Society of Environmental Engineers)
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Measuring Phosphatase Activity in Peatland Soils: Recent Methodological Advances

  • Freeman, Chris (School of Biological Sciences, University of Wales) ;
  • Jang, In-Young (School of Civil and Environmental Engineering, Yonsei University) ;
  • Zho, Kyoung-Duk (School of Public Health, Seoul National University) ;
  • Kang, Ho-Jeong (School of Civil and Environmental Engineering, Yonsei University)
  • 발행 : 2008.12.28
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초록

Measurements of phosphatase activity in peatlands are made difficult by the low levels of activity and the characteristically high concentrations of dissolved organic matter within the sediments. These materials may cause high background absorbances in colorimetric assays, and quenching interference in fluorimetric assays. This review describes the development of a new approach which allows such problems to be overcome by using HPLC to separate the interferences from the products of enzymic hydrolysis. This approach is applicable to various environmental samples such as peat, wetland sediment, and sludge which may contain a large amount of interfering organic matters.

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참고문헌

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피인용 문헌

  1. Innovative methods in soil phosphorus research: A review vol.178, pp.1, 2015, https://doi.org/10.1002/jpln.201400327