Journal of the Korean Society of Tobacco Science (한국연초학회지)

The Korean Society of Tobacco Science (한국연초학회)
권호 표지

Reduction in Salmonella mutagenicity of mainstream cigarette smoke condensate by cation exchange chromatography

  • Published : 2008.12.25
Download PDF
⟨ Previous Next ⟩

Abstract

Mutagenicity of cigarette smoke is one of the major health concerns related to smoking. Reduction of the components comprising mutagenic activity in cigarette mainstream smoke can be expected to bring about reduced risk of smoking. The purpose of this study is to isolate mutagenic compounds and to investigate the relative contribution to allover mutagenicity of smoke to find clues for the effective elimination of the components. Cigarette smoke condensate (CSC) was obtained from total particulate matter (TPM) of mainstream smoke, and several fractions fractionated from CSC were made by combination of cation exchange chromatograph and reverse-phase chromatography. The mutagenic activity of these fractions was assessed using Salmonella mutagenicity assay with S. typimurium TA98 strain in the presence of metabolic activation system (S-9). The fractions isolated by cation exchange and reverse-phase column showed relatively high mutagenic activity. The basic and hydrophilic fraction 9 showed approximately 33% of mutagenic activity of CSC and its specific activity was 2,459 revertants/mg TPM. These results suggest that hydrophilic cation exchanger and/or other adsorbents possessing similar properties may be used to remove the mutagenic compounds from mainstream smoke.

Keywords

References

  1. DeMarini, D. M. (2004) Genotoxicity of tobacco smoke and tobacco smoke condensate. Mutat. Res. 567: 447-474. https://doi.org/10.1016/j.mrrev.2004.02.001
  2. Dube, M. F. and Green, C. R. (1982) Method of collection of smoke for analytical purpose. Rec. Adv. Sci. 8: 42-102.
  3. Doolittle, D. J., Lee, C. K., Ivett, J. L., Mirsalis, J. C., Riccio, E., Rudd, C. J., Burger,G. T. and Hayes A. W. (1990) Comparative studies on the genotixic activity of smoke condendsate from cigarettes which burn or only heat tobacco. Enviromental and Molecular Mutagenesis 15: 93-105. https://doi.org/10.1002/em.2850150206
  4. Hoffmann, D., Hoffmann, I. and EI-Bayoumy, K. (2001) The less harmful cigarette: a controversial issue. Chem. Res. Toxicol. 14: 767-790. https://doi.org/10.1021/tx000260u
  5. Hutton, J. J. and Hackney, C. (1975) Metabolism of cigarette smoke condensates by human and rat homogenates to form mutagens detectable by Salmonella typhimurium TA1538. Cancer Res. 35: 2461-2468
  6. Kier, L. D., Yamasaki, E. and Ames, B. N.(1974) Detection of mutagenic activity in cigarette smoke condensates. Proc. Nat. Acad. Sci. USA 71: 4159-4163.
  7. Lauterbach, J. H. (2002) Smoke chemistry: A useful predictor of smoke toxicology. Rec. Adv. Tob. Sci. 28: 6-68.
  8. Maron, D. M. and Ames, B. N. (1983): Revised methods for the Salmonella. Mutat. Res. 113: 173-215. https://doi.org/10.1016/0165-1161(83)90010-9
  9. Manabe, S. and Wada, O. (1990) Carcinogenic tryptophan pyrolysis products insmoke conensate and cigarette smoke-polluted indoor air. Environ. Pollute. 64: 121-132. https://doi.org/10.1016/0269-7491(90)90109-P
  10. Rodgman, A. and Green, C. R. (2003) Toxic chemicals in cigarette mainstream smoke-hazard and hoopla. Beitragezur Tabakforschung 20: 481-545.
  11. Sato, S., Seino, Y., Ohka, T., Yahagi, T., Nagao, M., Matsushima, T. and Sugimura, T. (1977) Mutagenicity of smoke condensates from cigarettes, cigars and pipe tobacco. Cancer Lett. 3: 1-8, https://doi.org/10.1016/S0304-3835(77)93662-X
  12. Shin, H. J., Sohn, H. O., Park, C. H., Lee, H. S., Min,Y. K. and Hyun, H. C. (2007) Evaluation of the in vitro biological activity of selected 35 chemicals. J. of the Korean. Soc. of Tobacco Sci. 29: 30-40.
  13. Steele, RH, Payne, V. M., Fulp, C. W., Rees, D. C., Lee, C. K.and Doolittle, D. J. (1995) A comparison of the mutagenicity of mainstream cigarette smoke condensates from a representative sample of the U.S. cigarette market with a Kentucky reference cigarette (1R4F). Mutat. Res. 342: 179-190. https://doi.org/10.1016/0165-1218(95)90027-6
  14. Tewes, F. J., Meisgen, T. J., Veltel, D. J., Roemer, E. and Patskan, G. (2003) Toxicological evaluation of electrically heated cigarette. Part 3: genotoxicity and cytotoxicity of mainstream smoke. J. Appl. Toxicol. 23: 341-348. https://doi.org/10.1002/jat.925