Results of Embryo Transfer with Hanwoo Embryos Produced In-Vivo or In-Vitro to Holstein Cows as Recipients

체내 또는 체외에서 생산된 한우 수정란을 젖소 수란우에 이식한 결과

  • Kim, Yong-Jun (College of Veterinary Medicine, Chonbuk National University) ;
  • Park, Hoon (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Hae-Lee (Livestock Breeding Station, Jeonbuk Livestock Development & Research Institute) ;
  • Shin, Dong-Su (Livestock Breeding Station, Jeonbuk Livestock Development & Research Institute) ;
  • Jo, Sung-Woo (Livestock Breeding Station, Jeonbuk Livestock Development & Research Institute) ;
  • Kim, Yong-Su (Livestock Breeding Station, Jeonbuk Livestock Development & Research Institute) ;
  • Kim, Sue-Hee (College of Veterinary Medicine, Chonbuk National University)
  • 김용준 (전북대학교 수의과대학) ;
  • 박훈 (전북대학교 수의과대학) ;
  • 이해리 (전라북도 축산위생연구소 종축시험소) ;
  • 신동수 (전라북도 축산위생연구소 종축시험소) ;
  • 조성우 (전라북도 축산위생연구소 종축시험소) ;
  • 김용수 (전라북도 축산위생연구소 종축시험소) ;
  • 김수희 (전북대학교 수의과대학)
  • Published : 2008.09.30

Abstract

This study was performed to investigate the result that in-vivo or in-vitro embryos of Hanwoo cows were transferred to Holstein cows. Seventeen Hanwoo cows were used as donors for production of in-vivo embryos and fresh hanwoo in-vivo embryos were transferred to 1,150 Holsteins. And 2 embryos were transferred to 188 Holstein recipients to produce twin calves. Diagnosis on pregnancy was performed by rectal palpation at $60\sim90$ days after transfer. The pregnancy rate of Holstein recipients was 55.8% after transfer with Hanwoo in-vivo embryos and 38.2% after transfer with Hanwoo in-vitro embryos. The delivery rate of pregnant Holstein recipients was 88.4% after transfer with Hanwoo in-vivo embryos and 75.6% after transfer with Hanwoo in-vitro embryos. The rate of delivery of Holstein recipients transferred with two Hanwoo embryos was 36.2% and the rate of twin production was 25.9%. The rate of twin production by embryo transfer with in-vivo embryos was 30.4%, whereas the fate with in-vitro embryos was 15.6%. The pregnancy rate according to the grade of corpus luteum of Holstein recipients transferred with Hanwoo in-vitro embryos was 41.5 and 36.0% for A and B grade, respectively. The pregnancy rate according to the transfer in site in the uterine lumen of recipients was 40.9 and 32.7% for anterior and middle site, respectively. The pregnancy rate according to day of embryo transfer after estrus of recipients was 45.5, 38.8 and 39.7% for day 6, day 7 and day 8, respectively. There was difference of pregnancy rate according embryo transfer technician ($30.5\sim45.8%$) individual dairy farm ($21.1\sim51.0%$). These results are supposed to indicate that the rate of pregnancy after transfer with Hanwoo embryos to Holstein recipients was similar to that within the same breed, and consequently that this method would be beneficial to enhance the productivity in Hanwoo reproduction.

Keywords

References

  1. Ax RL, Armbrust S, TappanR, Gilbert G, Oyarzo JN, Bellin ME, Selner D, and McCauley TC. 2005. Superovulation and embryo recovery from peripubertal Holstein heifer. Anim. Reprod. Sci. 85:71-80 https://doi.org/10.1016/j.anireprosci.2004.04.034
  2. Block J, Drost M, Monson L, Ruteldge JJ, Rievera RM, Paula-Lopes FF, Ocon OM, Krininger CE, Liu J and Hansen PJ. 2003. Use of insulin-like growth factor-during embryo culture and treatment of recipients with gonadotropin-releasing hormone to increase pregnancy rates following the transfer of in vitro-produced embryos to heat- stressed. lactating cows. J. Anim. Sci. 81:1590-1602
  3. Brackett BG and Oliphant G. 1975. Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12:260-274 https://doi.org/10.1095/biolreprod12.2.260
  4. Fischer-Brown A, Crooks A, Leonard S, Monson R, Northey D and Rutleedge JJ. 2005. Parturition following transfer of embryos produced in two media under two oxygen concentrations. Theriogenology 87:215-228
  5. Greve T, Callesen H, Hyttel R and Assey R. 1995. The effects of exogenous gonadotropins. Theriogenology 46:41-50
  6. Hasler JF. 2001. Factors affecting frozen and fresh embryo transfer pregnancy rates in cattle. Theriogenology 56:1401-1415 https://doi.org/10.1016/S0093-691X(01)00643-4
  7. Isogai T, Nakanoshi T, Sasaki K and Tagami M. 1991. Twin pregnancy and births in cattle induced by FSH and PGF or PGF-analogue treatment. Jpn. J. Anim. Reprod. 37:105-113 https://doi.org/10.1262/jrd1977.37.105
  8. Lazzari G, Wrenzycki C, Herrmann D, Duchi R, Kruiop T, Niemann H and Galli C, 2002. Cellular and molecular deviations in bovine in vitro produced embryos are related to the large offspring syndrome. Biol. Reprod. 67:767-775 https://doi.org/10.1095/biolreprod.102.004481
  9. Misra Sk, Mutha Rao M, Jairaj R, Ranga Reddy NS and Pant HC 1999. Factors affecting pregnancy rate following nonsurgical embryo transfer in buffalo (Bubalis bubalis): A retrospective study. Theriogenology 52:1-10 https://doi.org/10.1016/S0093-691X(99)00105-3
  10. Numabe T, Oikawa T, Kikuchi T and Horiuchi T. 2000. Production efficiency of Japanese Black calves by transfer of produced in vitro. Theriogenology 54:1409-1420 https://doi.org/10.1016/S0093-691X(00)00463-5
  11. Numabe T, Oikawa T, Kikuchi T and Horiuchi T. 2000. Birth weight and birth rate of heavy calves conceived by transfer of in vitro or in vivo produced bovine embryos. Anim. Reprod. Sci. 64:13-20 https://doi.org/10.1016/S0378-4320(00)00190-1
  12. Reevers JJ, Chang CJ, Deavila DM, Johnson HE and Roberts AJ. 1989. Vaccines against endogenous hormones possible future tool in animal production. J. Dairy Sci. 72:3363-3371 https://doi.org/10.3168/jds.S0022-0302(89)79499-6
  13. Schmidt M, Greve T, Avery B, Beckers JF and Hansen HB. 1996. Pregnancies, calves, and calf viability after transfer of in vitro produced bovine embryos. Theriogenology 46: 527-539 https://doi.org/10.1016/0093-691X(96)00174-4
  14. Seidel GE. 1980. Critical review of embryo transfer procedures with cattle. Fert. and Embryo Develop. Plenum Press 323-353
  15. Spell AR, Beal WE, Corah LR and Lanb GC. 2001. Evaluating recipient and embryo factors that affect pregnancy rate of embryo transfer in beef cattle. Theriogenoogy 56:287-297 https://doi.org/10.1016/S0093-691X(01)00563-5
  16. Sreenan JM and Diskin MG. 1989. Effect of unilateral or bilateral twin embryo distribution on twining and embryo survival rate in the cow. J. Reprod. Fertil. 87:657-664 https://doi.org/10.1530/jrf.0.0870657
  17. Suzuki T, Geschi M, Yonai M and Sakaguchi M. 1994. Effects of method of embryo production and transfer on pregnancy rate, embryo survival rate. abortion and calf production in beef cows. Theriogenology 41:309(Abstr) https://doi.org/10.1016/S0093-691X(05)80219-5
  18. van Wagtendonk-de Leeuw AM, Aerts BJG and den Daas JHG. 1998. Abnormal offspring following in vitro production of bovine preimplantation embryos: a field study. Theriogenology 49:883-894 https://doi.org/10.1016/S0093-691X(98)00038-7
  19. 공일근, 2003. 한우 체외 수정란 이식 후 수태율 향상 방안. 한국수정란이식학회지 18(1) 부록:27-37
  20. 김덕임, 서상원, 정재경, 이규승, 서길웅, 박창식, 정영채, 박병권, 2002. 한우에 있어서 체내 수정란의 생산과 이식에 관한 연구; 한우 수정란 이식이 수태율에 미치는 요인, 한국수정란이식학회지 17; 33-44
  21. 김소섭, 최석화, 김재명, 이재협, 김재영, 박흠대. 2003. 한우 유래의 체외 수정란의 이식 후 임신에 관한 연구. 한국수정란이식학회지 18:237-242
  22. 김용권, 김진성. 2000. 한우 체외 수정란의 생산과 이식 후 쌍자생산에 관한 연구. 한국가축번식학회지 24: 97-108
  23. 김용준, 김희천, 서세현, 정구남, 김용수, 이해이, 신동수, 조성우, 김수희, 2005. 한우 및 젖소에서 체외 수정한 생산과 신선 및 동결 수정란 이식 결과. 한국수정란이식학회지 20: 79-87
  24. 김용준, 송재웅, 서세현, 정구남, 김용수, 이해이, 신동수, 조성우, 김수희, 2004. 한우 및 젖소에서 과배란처리를 이용한 체내 수정란 생산과 신선 및 동결 수정한 이식 결과. 한국수정란이식학회지 19:209-218
  25. 김홍률, 김덕임, 박노형, 김창근, 정영채, 윤종택, 전광주, 1998. 한우에서 수정란 이식의 효율 증진에 관한 연구. 한국수정란이식학회지 13:53-67
  26. 박용수, 2004. 한우 체내, 체외 및 복제 수정란 이식된 수란우의 임신과 분만 및 산자의 생존. 한국수정란이식학회지 19:239-244
  27. 박용수, 김소섭, 박흠대, 박현정, 김재명, 2005. 한우 체외 수정란이 이식된 수란우의 임신과 유산에 미치는 수정란측 요인. 한국수정란이식학회지 20:89-95
  28. 임석기, 우재석, 전기준, 장선식, 강수원, 윤상기, 손동수, 1998. 한우에 있어서 PEG네 용해시킨 Folltropin-V의 1회 피하주사에 의한 다배란 유기. 한국수정란이식학회지 13:207-212
  29. 조헌조, 김주현, 성상현, 2000. 한우 체외 수정란이식에 의한 산자 생산. 한국수정란이식학회지 15:225-261
  30. 최선호, 유일선, 손동수, 조상래, 한만희, 김현종, 최창용, 김영근, 2005. 한우의 반복 과배란 처리 및 산차가 수정란 생산에 미치는 영향. 한국수정란이식학회지 20:185-190
  31. 황한섭, 장현용, 김성곤, 김종택, 박춘근, 정희태, 김정익, 양부근, 2004. 한우의 체외성숙. 체외 수정란의 수정란이식에 관한 연구. 한국수정란이식학회지 19:1-10