Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Inhibitor of DNA Binding Protein (Id)1 and Id2 mRNA Expression on Folliculogenesis in Rat Ovary

랫드 난소에서 난포 발달에 따른 DNA 결합 단백질 억제인자 (Inhibitor of DNA Binding Protein) Id1 and Id2 mRNA 발현

  • 황성수 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 김평희 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 고응규 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 양병철 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 성환후 (농촌진흥청 축산과학원 응용생명공학과) ;
  • 민관식 (한경대학교 생물.환경정보통신전문대학원) ;
  • 윤종택 (한경대학교 동물자원과학과)
  • Published : 2008.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to analyze the expression pattern of inhibitor of DNA binding proteins (Id)1 and Id2 mRNA on folliculogenesis in rat ovary. The ovaries were obtained from 27 days old Sprague-Dawley rat, fixed, dehydrated, and paraffin embedded. For in situ hybridization, anti-sense and sense Idl and Id2 cRNA probes were prepared and applied to the ovarian section. The ovarian sections were coated with NTB-2 emulsion. After that, the slides were developed and counterstained with hematoxylin and eosin staining. In oocytes, the hybridizational signals of Id1 mRNA were strong in primordial and primary follicles, however, there were no signals in that of atretic or preovulatory follicles. The Id2 mRNA signals were also strong in the oocytes of primordial, primary and secondary follicles. Interestingly, the Id2 mRNA was expressed specifically granulosa cells, but nor in oocyte or theca cells in dominant and preovulatory follicles. Based on these results, Id1 and Id2 mRNA was expressed specifically at follicle stages and follicular tissue and might be closely related with follicle development.

Keywords

References

  1. Benezra R, Rafii S and Lyden D. 2001. The Id proteins and angiogenesis. Oncogene 20:8334-41 https://doi.org/10.1038/sj.onc.1205160
  2. Erickson GF and Shimasaki S. 2000. The role of the oocyte in folliculogenesis. Trends Endocrinol. Metab. 11:193-198 https://doi.org/10.1016/S1043-2760(00)00249-6
  3. Fortune JE. 2003. The early stages of follicular development: activation of primordial follicles and growth of preantral follicles. Anim. Reprod. Sci. 78:135-163 https://doi.org/10.1016/S0378-4320(03)00088-5
  4. Gougeon A. 1996. Regulation of ovarian follicular development in primates: facts and hypotheses. Endocr. Rev. 17: 121-155 https://doi.org/10.1210/edrv-17-2-121
  5. Hollnagel A, Oehlmann V, Heymer J, Rüther U and Nordheim A. 1999. Id genes are direct targets of bone morphogenetic protein induction in embryonic stem cells. J. Biol. Chem. 274:19838-19845 https://doi.org/10.1074/jbc.274.28.19838
  6. Hourvitz A, Kuwahara A, Hennebold JD, Tavares AB, Negishi H, Lee TH, Erickson GF and Adashi EY. 2002. The regulated expression of the pregnancy-associated plasma protein- A in the rodent ovary: a proposed role in the development of dominant follicles and of corpora lutea. Endocrinology 143:1833-1844 https://doi.org/10.1210/en.143.5.1833
  7. Howe HL, Wingo PA, Thun MJ, Ries LA, Rosenberg HM, Feigal EG and Edwards BK. 2001. Annual report to the nation on the status of cancer (1973 through 1998), featuring cancers with recent increasing trends. J. Natl. Cancer Inst. 93: 824-842 https://doi.org/10.1093/jnci/93.11.824
  8. Hwang SS, Choi EJ, You SK, Choi YJ, Min KS and Yoon JT. 2006. Development of bovine nuclear transfer embryos using life-span extended donor cells transfected with foreign gene. Asian-Aust. J. Anim. Sci. 19:1574-1579
  9. Im GS, Seo JS, Hwang IS, Kim DH, Kim SW, Yang BC, Yang BS, Lai L and Prather RS. 2006. Development and apoptosis of pre-implantation porcine nuclear transfer embryos activated with different combination of chemicals. Mol. Reprod. Dev. 73:1094-1101 https://doi.org/10.1002/mrd.20455
  10. Johnson AL, Haugen MJ and Woods DC. 2008. Role for inhibitor of differentiation/deoxyribonucleic acid-binding (Id) proteins in granulosa cell differentiation. Endocrinology 149: 3187-3195 https://doi.org/10.1210/en.2007-1659
  11. Knight PG and Glister C. 2006. TGF-beta superfamily members and ovarian follicle development. Reproduction 132: 191-206 https://doi.org/10.1530/rep.1.01074
  12. Lasorella A, Uo T and Iavarone A. 2001. Id proteins at the crossroad of development and cancer. Oncogene 20:8326-8333 https://doi.org/10.1038/sj.onc.1205093
  13. Lee HJ, Hwang SS and Yoon JT. 2007. Effects of bovine somatotropin (bST) administration combined with controlled internal drug release (CIDR) on embryo quality and pregnancy of Hanwoo (Korean native beef cattle) during commercial embryo transfer program. Asian-Aust. J. Anim. Sci. 20:194-199
  14. Massari ME and Murre C. 2000. Helix-loop-helix proteins: regulators of transcription in eucaryotic organisms. Mol. Cell Biol. 20:429-440 https://doi.org/10.1128/MCB.20.2.429-440.2000
  15. Matsui M, Sonntag B, Hwang SS, Byerly T, Hourvitz A, Adashi EY, Shimasaki S and Erickson GF. 2004. Pregnancyassociated plasma protein-a production in rat granulosa cells: stimulation by follicle-stimulating hormone and inhibition by the oocyte-derived bone morphogenetic protein-15. Endocrinology 145:3686-3695 https://doi.org/10.1210/en.2003-1642
  16. Matsumura ME, Lobe DR and McNamara CA. 2002. Contribution of the helix-loop-helix factor Id2 to regulation of vascular smooth muscle cell proliferation. J. Biol. Chem. 277: 7293-7297 https://doi.org/10.1074/jbc.M108986200
  17. McGee EA and Hsueh AJ. 2000. Initial and cyclic recruitment of ovarian follicles. Endocr. Rev. 21(2):200-14 https://doi.org/10.1210/er.21.2.200
  18. Miyazono K and Miyazawa K. 2002. Id: A Target of BMP Signaling. Sci. STKE. 151. pe40
  19. Nakashima K, Takizawa T, Ochiai W, Yanagisawa M, Hisatsune T, Nakafuku M, Miyazono K, Kishimoto T, Kageyama R and Taga T. 2001. BMP2-mediated alteration in the developmental pathway of fetal mouse brain cells from neurogenesis to astrocytogenesis. Proc. Natl. Acad. Sci. U.S.A. 98:5868-5873
  20. Norton JD. 2000. ID helix-loop-helix proteins in cell growth, differentiation and tumorigenesis. J. Cell Sci. 113:3897-3905
  21. Park JK, Lee YK, Lee PY, Chung HJ, Kim SW, Lee HG, Seo MK, Han JH, Park CG, Kim HT, Kim YK, Min KS, Kim JH, Lee HT and Chang WK. 2006. Recombinant human erythropoietin produced in milk of transgenic pigs. J. Biotech. 122:362-371 https://doi.org/10.1016/j.jbiotec.2005.11.021
  22. Shimasaki S, Moore RK, Otsuka F and Erickson GF. 2004. The bone morphogenetic protein system in mammalian reproduction. Endocr. Rev. 25:72-101 https://doi.org/10.1210/er.2003-0007
  23. Son DS, Choe CY, Cho SR, Choi SH, Kim HJ, Lee JI and Kim IH. 2008. Timed artificial insemination or embryo transfer using CIDR, estradiol benzoate, and prostaglandin F2 for the rebreeding of Korean native donor cettle. J. Emb. Trans. 23:81-86
  24. Springhorn JP, Singh K, Kelly RA and Smith TW. 1994. Posttranscriptional regulation of Id1 activity in cardiac muscle. Alternative splicing of novel Id1 transcript permits homodimerization. J. Biol. Chem. 269:5132-5136
  25. Yang BC, Im GS, Kim DH, Yang BS, Oh HJ, Park HS, Seong HH, Kim SW, Ka HH and Lee CK. 2008. Development of vitrified-thawed bovine oocytes after in vitro fertilization and somatic cell nuclear transfer. Anim. Reprod. Sci. 103: 25-37 https://doi.org/10.1016/j.anireprosci.2006.12.009
  26. Yang Y, Wang HC, Sun XH. 2008. Id1 induces apoptosis through inhibition of ROR gammat expression. BMC Immunol. 9: 20 https://doi.org/10.1186/1471-2172-9-20
  27. Yokota Y. 2001. Id and development. Oncogene. 20:8290-8298 https://doi.org/10.1038/sj.onc.1205090
  28. 황성수, 고응규, 임현주, 성환후, 윤종택, 민관식, 2007. 랫드 난소에서 난포발달에 따른 Id3 mRNA의 발현, 한국동물번식학회지 31:181-186