Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Comparison of 5 Staining Methods for Somatic Cells in Dairy Goat Milk Samples

젖염소 유즙에 적용한 5가지 체세포 염색 방법의 비교

  • Kim, Young-Chul (College of Veterinary Medicine, Chonnam National University) ;
  • Park, Ha-Yeon (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Youn-Kyung (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Jeong-Chi (Department of clinical pathology, Gwangju health college) ;
  • Su, Guk-Hyun (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Chai-Yong (College of Veterinary Medicine, Chonnam National University)
  • 김영철 (전남대학교 수의과대학) ;
  • 박하연 (전남대학교 수의과대학) ;
  • 이윤경 (전남대학교 수의과대학) ;
  • 이정치 (광주보건대학 임상병리과) ;
  • 서국현 (전남대학교 수의과대학) ;
  • 이채용 (전남대학교 수의과대학)
  • Published : 2008.08.31
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Abstract

This study was performed to investigate the best staining method for the somatic cell classification of dairy goat milk. Dairy goat milk samples, which were collected randomly from a dairy goat farm in Jeollanam-do, South Korea, were stained and analyzed with direct microscopic method, using 5 different staining methods; Wright's stain, Giemsa stain, Diff-quik stain, Newman's stain and Pyronin Y-Methyl Green stain, respectively. Among them, The Newman's staining was found to be the most rapid and effective method, for it required the shortest time for staining and provided the easiest way to classify somatic cells.

Keywords

References

  1. 강성규, 이동배, 이영수. 유기용제의 위해도 평가 및 일부 유기용제의 생물학적 폭로지표. 충남의대잡지 1993; 20: 113-134
  2. 김혜라, 이정치, 정지영, 이윤경, 신성식, 이채용. 젖염소 분방유즙에서 체세포에 대한 연구. 한국수의임상학회지 2007; 24: 177-181
  3. 윤준철, 이정치, 김상기, 박영석, 김종택, 이정길, 이채용. 젖염소 분방유즙에서 분리한 세균 및 항균제 감수성조사. 대한수의학회지 2004; 44: 151-157
  4. 이삼열, 정윤섭. 임상병리검사법. 3판. 서울: 연세대학교출판부. 1987: 102
  5. 함준상, 정석근, 인영민, 김동운, 김용곤, 김현욱. 영동군에 서 생산된 자아넨 산양유의 특성. 한국축산식품학회 23차 학술발표 1999: 195
  6. Deitch AD. A method for the cytophotometric estimation of nucleic acids using methylene blue. J Histochem Cytochem. 1964; 12: 451-461 https://doi.org/10.1177/12.6.451
  7. Dulin AM, Paape MJ, Wergin WP. Differentiation and enumeration of somatic cells in goat milk. J Food Prot 1982; 45: 435-439 https://doi.org/10.4315/0362-028X-45.5.435
  8. Gonzalo C, Martinez JR, Carriedo JA, Primitivo FS. Fossomatic Cell-Counting on ewe milk: comparison with direct microscopy and study of variation factors. J Dairy Sci 2003; 86: 138-145 https://doi.org/10.3168/jds.S0022-0302(03)73593-0
  9. Gurr E. A practical manual of medical and biological staining techniques. 2nd ed. New York: Interscience Publishers. 1956: 387-388
  10. Haenlein GFW. Past, present, and future perspectives of small ruminant dairy research. J Dairy Sci 2001; 84: 2097-2115 https://doi.org/10.3168/jds.S0022-0302(01)74655-3
  11. Horobin RW, Walter1 KJ. Understanding romanowsky staining. Histochem Cell Biol 1987; 86: 331-336 https://doi.org/10.1007/BF00490267
  12. Jenness R. Composition and characteristics of goat milk. J Dairy Sci 1980; 63: 1605-1630 https://doi.org/10.3168/jds.S0022-0302(80)83125-0
  13. Kaufmann BP, Mcdonald M, Gay H. Enzymatic degradation of ribonucleoproteins of chromosomes, nucleoli and cytoplasm. Nature 1948; 162: 814 https://doi.org/10.1038/162814a0
  14. Ljutovac KR, Pirisi A, Cremoux RD and Gonzalo C. Somatic cells of goat and sheep milk: Analytical, sanitary, productive and technological aspects. 2007; 68: 126-144 https://doi.org/10.1016/j.smallrumres.2006.09.012
  15. Maisi P. Analysis of physiological changes in caprine milk with CMT, NAGase and anti-trypsin. Small Rumin Res 1990; 3: 485-492 https://doi.org/10.1016/0921-4488(90)90079-L
  16. Paape MJ, Capuco AV. Cellular defense-mechanisms in the udder and lactation of goats. J Anim Sci 1997; 75: 556-565
  17. Packard VS, Tatini S, Fugua R, Heady J, Gilman C. Direct microscopic methods for bacterial or somatic cells. In: Standard methods for the examination of dairy products, 16th ed. Washington DC: Marshall RT. 1992; 309?325
  18. Sanchez A, Sierra D, Luengo C, Corrales JC, Morales CT, Contreras A, Gonzalo C. Influence of storage and preservation on fossomatic cell count and composition of goat milk. J Dairy Sci 2005; 88: 3095-3100 https://doi.org/10.3168/jds.S0022-0302(05)72991-X
  19. Schalm OW, Caroll ES, Jain NC. Bovine mastitis. 1st ed. Philadelphia: Lea and Fiebiger. 1971: 113-127
  20. Selvi E, Manganelli S, Catenaccio M, Stefano RD, Frati E, Cucini S, Marcolongo R. Diff-Quik staining method for detection and identification of monosodium urate and calcium pyrophosphate crystals in synovial fluid. Ann Rheum Dis 2001; 60: 194-198 https://doi.org/10.1136/ard.60.3.194
  21. Wooding FB, Peaker PM, Linzell JL. Theories of milk secretion: Evidence from the electron microscopic examination of milk. Nature 1970; 226: 762-764 https://doi.org/10.1038/226762a0
  22. Young CC, Breed RS. The newman combined fat extraction, fixing and staining solution for use in the direct microscopic technic for coutig bacteria in milk. Am J Public Health 1929; 19: 99-100 https://doi.org/10.2105/AJPH.19.1.99