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Diversity of PthA Gene of Xanthomonas Strains Causing Citrus Bacterial Canker and its Relationship with Virulence

  • Lee, Seung-Don (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Jung-Hee (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Dong-Hee (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Yong-Hoon (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA)
  • 발행 : 2008.09.30

초록

Several pathotypes have been recognized in citrus bacterial canker, which causing serious damage in citrus cultivation area. To control the disease, it is important to understand the pathological diversity and reason of difference in virulence of the causal pathogen. We analyzed 124 strains of Xanthomonas causing citrus bacterial canker by southern hybridization with an internal 3.4-kb BamHI fragment from pthA gene. Assuming each band represented an intact gene, each strain of Xanthomonas was estimated to have approximately 1 to 4 copies of pthA gene. X. a. pv. citri A type had more than 3 copies of pthA gene, and the number of pthA gene in X. a. pv. citri $A^*,\;A^w$, and X. a. pv. aurantifolii B, C were different from 1 to 3 according to the strains. When the pthA gene profile was classified into 13 groups according to the number and size of hybridization bands, most of the A types belong to the 3A group, and 4A and 4B type was dominant when they had 4 bands. However, there was no general pattern of difference between the virulence and pthA gene group in this test.

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참고문헌

  1. Bonas, U., Conrads-Strauch, J. and Balbo, I. 1993. Resistance in tomato to Xanthomonas campestris pv. vesicatoria is determined by alleles of the pepper-specific avirulence gene avrBs3. Mol. Gen. Genet. 238:261-269
  2. da Silva, A. C. R., Ferro, J. A. and Reinach, F. C. et al. 2002. Comparison of the genomes of two Xanthomonas pathogens with differing host specificities. Nature 417:459-463. 2002 https://doi.org/10.1038/417459a
  3. Flor, H. H. 1955. Host-parasite interaction in flax rust - its genetics and other implications. Phytopathology 45:680-685
  4. Fujikawa, T., Ishihara, H., Leach, J. E. and Tsuyumu, S. 2006. Suppression of defense response in plants by the avrBs3/pthA gene family of Xanthomonas spp. Mol. Plant Microbe. Interact. 19:342-349 https://doi.org/10.1094/MPMI-19-0342
  5. Graham, J. H., Gottwald, T. R., Cubero, J. and Achor, D. S. 2004. Xanthomonas axonopodis pv. citri: factors affecting successful eradication of citrus canker. Mol. Plant. Pathol. 5:1-15 https://doi.org/10.1046/j.1364-3703.2004.00197.x
  6. Herbers, K., Conrads-Strauch, J. and Bonas, U. 1992. Race-specificity of plant resistance to bacterial spot disease determined by repetitive motifs in a bacterial avirulence protein. Nature 356:172-174 https://doi.org/10.1038/356172a0
  7. Kingsley, M. T., Gabriel, D. W., Marlow, G. C. and Roberts, P. 1993. The opsX Locus of Xanthomonas campestris affects host range and biosynthesis of lipopolysaccharide and extracellular polysaccharide. J. Bacteriol. 175:5839-5850 https://doi.org/10.1128/jb.175.18.5839-5850.1993
  8. Lee, Y. H., Lee, S., Lee, D. H., Ji, S. H., Chang, H. Y., Heu, S., Hyun, J. W., Ra D.-S. and Park, E. W. 2008. Differentiation of citrus bacterial canker strains in Korea by host range, rep-PCR fingerprinting and 16S rDNA analysis. Eur. J. Plant Pathol. 121:97-102 https://doi.org/10.1007/s10658-007-9242-5
  9. Schornack, S., Minsavage, G. V., Stall, R. E., Jones, J. B. and Lahaye, T. 2008. Characterization of AvrHah1, a novel AvrBs3-like effector from Xanthomonas gardneri with virulence and avirulence activity. New Phytol. 179:546-556 https://doi.org/10.1111/j.1469-8137.2008.02487.x
  10. Swarup, S., De Feyter, R., Brlansky, R. H. and Gabriel, D. W. 1991. A pathogenicity locus from Xanthomonas citri enables strains from several pathovars of X. campestris to elicit canker like lesions on citrus. Phytopathology 81:802-809 https://doi.org/10.1094/Phyto-81-802
  11. Swarup, S., Yang, Y., Kingsley, M. T. and Gabriel, D. W. 1992. A Xanthomonas citri pathogenicity gene, pthA, pleiotropically encodes gratuitous avirulence on nonhosts. Mol. Plant-Microbe Interact. 5:204-213 https://doi.org/10.1094/MPMI-5-204
  12. Wu, X. M., Li, Y. R., Zou, L. F. and Chen, G. Y. 2007. Gene-forgene relationships between rice and diverse avrBs3/pthA avirulence genes in Xanthomonas oryzae pv. oryzae. Plant Pathology 56:26-34
  13. Yang, Y., De Feyter, R. and Gabriel, D. W. 1994. Host-specific symptoms and increased release of Xanthomonas citri and X. campestris pv. malvacearum from leaves are determined by the 102-bp tandem repeats of pthA and avrb6, respectively. Mol. Plant-Microbe Interact. 7:345-355 https://doi.org/10.1094/MPMI-7-0345
  14. Yang, Y. and Gabriel, D. W. 1995. Intragenic recombination of a single plant pathogen gene provides a mechanism for the evolution of new host specificities. J. Bacteriol. 177:4963-4968 https://doi.org/10.1128/jb.177.17.4963-4968.1995

피인용 문헌

  1. tale-Based Genetic Diversity of Chinese Isolates of the Citrus Canker Pathogen Xanthomonas citri subsp. citri vol.97, pp.9, 2013, https://doi.org/10.1094/PDIS-12-12-1201-RE
  2. Genetic structure analysis of strains causing citrus canker in Iran reveals the presence of two different lineages ofXanthomonas citripv.citripathotype A* vol.64, pp.4, 2015, https://doi.org/10.1111/ppa.12324