Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of New Calcium Supplementary Food Containing Fermented Product of Bacillus on the Longitudinal Bone Growth in the Adolescent Male Rats

바실러스 발효대사물이 함유된 신규 칼슘보충용식품의 성장기 쥐 장골 성장촉진효과

  • 이재연 ((주)바이오토피아 부설생명과학연구소) ;
  • 박영식 ((주)바이오토피아 부설생명과학연구소) ;
  • 김영훈 (수원대학교 생명공학과) ;
  • 오경환 (수원대학교 생명공학과) ;
  • 황교열 ((주)바이오토피아 부설생명과학연구소) ;
  • 조용석 ((주)바이오토피아 부설생명과학연구소) ;
  • 강경돈 ((주)바이오토피아 부설생명과학연구소) ;
  • 김근 (수원대학교 생명공학과) ;
  • 주동관 ((주)엠에스바이오텍) ;
  • 성수일 ((주)바이오토피아 부설생명과학연구소)
  • Published : 2008.12.31
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Abstract

This study was conducted to investigate the effect of administration of calcium supplementary food containing fermented product of Bacillus subtilis SE4 highly producing poly-$\gamma$-glutamic acid on the growthparameters of adolescent male rats. Four-week old male Sprague-Dawley (SD) rats were fed for 4 weeks and assigned to the following 4 groups: two groups administered orally with new calcium supplementary food (such as 150 mg/kg and 300 mg/kg) containing fermented product of B. subtilis SE4, one group administered with conventional calcium supplementary food product (150 mg/kg) and one saline group as control. Daily weight gain and daily food intake in the two new food product groups were higher than those of conventional food product group and control group. Especially, the content of serum IGF-I in the two new food product groups were significantly higher than those in conventional food product group and in control group (p<0.05). In addition, length and weight of longitudinal bone in the two new food product groups were longer and heavier than those of conventional food product group and control group. Therefore, the addition of fermented food product of B. subtilis SE4 into the conventional calcium supplementary food increased all the parameters examined for the growth of the adolescent male rats.

기존 칼슘보충용식품의 성장촉진효능을 증진시키기 위하여 칼슘흡수 관여 물질인 $\gamma$-PGA를 다량 생산하는 Bacillus subtilis SE4 균주를 선발하였다. 선발된 B. subtilis SE4의 발효대사물을 기존 칼슘보충용식품에 첨가하여 성장기 수컷 흰쥐에 28일간 경구투여 한 결과 발효대사물이 첨가된 신규칼슘보충용식품군에서 혈청 내 IGF-I 함량이 대조군이나 기존칼슘보충용식품군에 비해 유의적으로 높게 나타났다(p<0.05). 일일증체량, 일일사료섭취량, 장골길이, 장골 무게, 장골폭 등의 성장관련 지표에서도 장골의 길이와 일부 부위별 두께에서의 유의적 결과를 포함하여 전반적으로 신규칼슘보충용식품군이 대조군과 기존칼슘보충용식품군에 비해 양호한 성적을 나타내었다. 이러한 결과는 기존칼슘 보충용 식품에 발효대사물을 첨가함으로써 나타난 칼슘의 생체흡수율 증대 및 성장호르몬의 분비활성화 등 여러 성장 관련요인들이 흰쥐 성장에 긍정적으로 작용한 때문으로 생각되어진다.

Keywords

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