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Changes in Solubility of Barley Arabinoxylans during Malting

보리의 제맥과정 중 Arabinoxylan의 용해성 변화

  • Eom, Hye-Seon (Dept. of Food Science and Biotechnology, Kyungwon University) ;
  • Lee, Young-Tack (Dept. of Food Science and Biotechnology, Kyungwon University)
  • 엄혜선 (경원대학교 식품생물공학과) ;
  • 이영택 (경원대학교 식품생물공학과)
  • Published : 2008.12.31

Abstract

Barleys at different malting stages from steeping to 5 day germination were investigated for soluble, insoluble, and total arabinoxylans at three different extraction temperatures of 21, 45 and $65^{\circ}C$. Slight differences in total arabinoxylan levels in barleys were observed during malting stages. During germination, initially insoluble arabinoxylan could be more soluble, thus the solubilized arabinoxylan tended to increase until 4 day germination. The proportion of soluble arabinoxylan in germinating barleys was increased from ambient temp ($21^{\circ}C$) to 45 or $65^{\circ}C$. Two barley malt samples were selected at two different stages of germination, well-modified malt germinated for 96 hr and poorly-modified malt for 60 hr, and mashed isothermally at 45, 55, 65, or $75^{\circ}C$ for 2 hr. Increasing temperature over 45 to $75^{\circ}C$ slightly decreased the amount of arabinoxylan solubilized in wort. Arabinoxylan content of wort from well-modified malt was not significantly different from poorly- modified malt at all mashing temperatures.

보리를 침맥한 후 5일 동안 발아하는 제맥과정 중에 arabinoxylan 함량의 변화를 측정하였다. 제맥과정 중 초기에 불용성인 보리의 arabinoxylan은 발아과정 중에 가용화됨에 따라 수용성 arabinoxylan의 함량이 약간 증가하는 추세를 보여주었다. 발아중인 보리의 수용성 arabinoxylan 부분은 $21^{\circ}C$ 추출온도에 비해 $45^{\circ}C$의 추출온도에서 보다 높은 함량 으로 나타났다. 발아시간에 따라 적절히 변형된 맥아(96시간 발아)와 덜 변형된 맥아(60시간 발아)를 선발하여 변형정도 에 따른 두 가지 맥아에 대하여 당화온도별로($45{\sim}75^{\circ}C$) 당 화 후 맥즙의 arabinoxylan 함량을 분석하였다. 당화온도를 $45^{\circ}C$에서 $75^{\circ}C$를 증가시킴에 따라 맥즙에 용출되어져 나오는 arabinoxylan의 함량은 약간 감소하는 경향을 보였다. 본 실험의 당화조건에서 적절히 변형된 맥아로부터 얻은 맥즙과 덜 변형된 맥아로부터 얻은 맥즙 간의 arabinoxylan 함량에서 큰 차이를 나타내지 않았다.

Keywords

References

  1. Fincher GB. 1975. Morphology and chemical composition of barley endosperm cell walls. J Inst Brew 81: 116-122 https://doi.org/10.1002/j.2050-0416.1975.tb03672.x
  2. Lethonen M, Aikasalo R. 1987. Pentosans in barley varieties. Cereal Chem 64: 133-134
  3. Henry RJ. 1986. Genetic and environmental variation in the pentosan and ${\beta}$-glucan contents of barley and their relation to malting quality. J Cereal Sci 4: 269-277 https://doi.org/10.1016/S0733-5210(86)80029-7
  4. Henry RJ. 1988. The carbohydrates of barley grains-a preview. J Inst Brew 94: 71-78 https://doi.org/10.1002/j.2050-0416.1988.tb04560.x
  5. Bamforth CW. 1994. ${\beta}$-Glucan and ${\beta}$-glucanases in malting and brewing: practical aspects. Brew Dig 69: 12-21
  6. Banik M, Li CD, Langridge P, Fincher GB. 1997. Structure, hormonal regulation, and chromosomal location of genes encoding barley (1→4)-${beta}$-xylan endohydrolases. Mol Gen Genet 253: 599-608 https://doi.org/10.1007/s004380050362
  7. Voragen AGJ, Schols HA, Marius J, Rombouts FM, Angelino SAGF. 1987. Non-starch polysaccharides from barley: structural features and breakdown during malting. J Inst Brew 93: 202-208 https://doi.org/10.1002/j.2050-0416.1987.tb04499.x
  8. Bamforth CW. 1982. Barley $\beta$-glucans: their role in malting and brewing. Brew Dig 3: 22-35
  9. Coote N, Kirsop BH. 1976. A haze consisting largely of pentosan. J Inst Brew 82: 34 https://doi.org/10.1002/j.2050-0416.1976.tb03718.x
  10. Edney MJ, LaBerge DE, Langrell DE. 1998. Relationships among the $\beta$-glucan contents of barley, malt, malt congress extract, and beer. J Am Soc Brew Chem 56: 164-168 https://doi.org/10.1094/ASBCJ-56-0164
  11. Han JY, Schwarz P. 1996. Arabinoxylan composition in barley, malt, and beer. J Am Soc Brew Chem 54: 216-220 https://doi.org/10.1094/ASBCJ-54-0216
  12. Li Y, Lu J, Gu G, Shi Z, Mao Z. 2005. Studies on water-extractable arabinoxylans during malting and brewing. Food Chem 93: 33-38 https://doi.org/10.1016/j.foodchem.2004.08.040
  13. American Society of Brewing Chemists. 1992. Methods of Analysis. 8th ed. Malt-4. The ASBC, St. Paul, MN
  14. European Brewery Convention. 1998. Analytica-EBC. 5th ed. Fachverlag Hans Carl, Nurnberg, Germany
  15. Blakeney AB, Harris PJ, Henry RJ, Stone BA. 1983. A simple and rapid preparation of alditol acetates for monosaccharide analysis. Carbohyd Res 113: 291-299 https://doi.org/10.1016/0008-6215(83)88244-5
  16. Douglas SG. 1981. A rapid method for the determination of pentosans in wheat flour. Food Chem 7: 139-145 https://doi.org/10.1016/0308-8146(81)90059-5
  17. Bamforth CW, Kanauchi M. 2001. A simple model for the cell wall of the starchy endosperm in barley. J Ins Brew 107: 235-240 https://doi.org/10.1002/j.2050-0416.2001.tb00095.x
  18. Kuntz RJ, Bamforth CW. 2007. Time course for the development of enzymes in barley. J Inst Brew 113: 196-205 https://doi.org/10.1002/j.2050-0416.2007.tb00276.x
  19. Aman P, Graham H. 1987. Analysis of total and insoluble mixed-linked ($1{\to}3$),($1{\to}4$)-$\beta$-D-glucans in barley and oats. J Agric Food Chem 35: 704-709 https://doi.org/10.1021/jf00077a016
  20. Izydorczyk MS, Macri LJ, MacGregor AW. 1998. Structural and physicochemical properties of barley non-starch polysaccharides II. Alkali-extractable B-glucans and arabinoxylans. Carbohydr Poly 35: 249-258 https://doi.org/10.1016/S0144-8617(97)00136-7
  21. Lee YT. 2008. Effects of malt modification on ${\beta}$-glucan solubility and beer viscosity. Korean J Food Sci Technol 40: 360-363
  22. Wang J, Zhang G, Chen J, Wu F. 2004. The changes of ${\beta}$-glucan content and ${\beta}$-glucanase activity in barley before and after malting and their relationships to malt qualities. Food Chem 86: 223-228 https://doi.org/10.1016/j.foodchem.2003.08.020

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