Effects of Extraction Conditions on the Componential Extraction of Brown Seaweed (Undaria pinnatifida)

  • Published : 2008.12.31


In order to improve the market competitiveness of the processed products of Undaria pinnatifida, various extraction conditions of Undaria pinnatifida were examined to determine the optimal conditions for the extraction of its valuable components. The highest level of alginic acid content was detected after 90 min of typical extraction or after 20 min of pressure extraction, after which the levels decreased slightly. The concentrations of reducing sugar and crude protein were also high after 90 min of typical extraction. Both alginic acid and reducing sugar were extracted in greater amounts using pressure extraction. The reducing sugar content was $2.8{\sim}3.2$ times higher using pressure extraction rather than typical extraction. Furthermore, the extraction results were superior with the, pressure extraction method. The appropriate temperature and duration of extraction were found to be $120^{\circ}C$ for 20 min. The use of a single enzyme yielded better results during extraction compared to the use of a mixture of enzymes. 20 min of pressure extraction followed by the addition of 0.1% pectinase and 2 hr of further extraction at $50^{\circ}C$ yielded high contents of alginic acid and reducing sugar from Undaria pinnatifida.


  1. Haug A, Larson B, Smidsrod O. 1966. A study of the constitution of alginic acid by partial acid hydrolysis. Acta Chem Scand 20: 183-190
  2. Hirst EL, Percival E, Wold JK. 1964. The structure of alginic acid. Part Partial hydrolysis of the reduced polysaccharide. J Chem Soc 8: 1493-1499
  3. Ito K, Tsuchiya Y. 1972. The effect of algal polysaccharides on the depressing of plasma cholesterol levels in rat. In Proc. of 7th Int Seaweed Symp. Nishizawa K, ed. Univ. Tokyo Press, Tokyo, Japan. p 558-561
  4. Park YH. 1969. Seasonal variation in the chemical composition of brown algae with special reference to alginic acid. J Korean Fish Soc 2: 71-82
  5. Kim CY, Park YH. 1975. Alginic acid contents in brown alga. Bull Natl Fish Univ (Busan) 15: 27-30
  6. Martin G. 1982. Food hydrocolloids. CRC Press, NY, USA. p 219-228
  7. Nishide E, Kinoshita Y, Anzai H, Uchida N. 1988. Distribution of hot-water extractable material, water-soluble alginate and alkali-soluble alginate in different parts of brown seaweed. Nippon Suisan Gakkaishi 54: 1619-1622
  8. Nishide E, Anzai H, Uchida NA. 1987. Comparative investigation on the water-soluble and the alkali-soluble alginates from various Japanese brown algae. Nippon Suisan Gakkaishi 53: 1215-1219
  9. Fujihara M, Nagumo T. 1989. Determination of the molecular weight of alginates by agrose gel filtration. J Chromatogr 465: 386-389
  10. Noda K, Takada K. 1983. Mannuronic to guluronic acid rations of alginic acid prepared from various brown seaweeds. Bull Jpn Soc Fish 49: 1591-1593
  11. Cho SY, Kang HJ, Joo DS, Lee JS, Kim SM. 1999. A comparative study on physical properties and gel formation abilities of hot-water extractable material, watersoluble alginate and alkali-soluble alginate extracted from Laminaria japonica in East Sea, Korea. J Korean Fish Soc 32: 774-778
  12. Han J, Kang S, Choue R, Kim H, Leem K, Chung S, Kim C, Chung J. 2002. Free radical scavenging effect of Diospyros kaki, Laminaria japonica and Undaria pinntifida. Fitoterapia 73: 710-712
  13. Kim KH, Kim CS. 1982. Studies on the manufacture of Undaria pinntifida Laver and it's physicochemical properties. Korean J Food Sci Technol 14: 336-341
  14. Kim KH, Kim CS. 1983. Studies on the manufacture of Undaria pinntifida Laver and its physicochemical properties (II). Korean J Food Sci Technol 15: 277-281
  15. Kim YD, Kim YM, Kang TS. 1981. Study on versatile utilization of seaweeds. Korea advanced food research institute research report. p 86-88
  16. Seo KB, Choi DY, Yoon SH. 1975. Processing experiment using seaweeds. Korea advanced food research institute research report. p 105
  17. Jo KS, Do JR, Koo JG. 1998. Pretreatment conditions of Porphyra yezoensis, Undaria pinnatifida and Laminaria religiosa for functional algae-tea. J Korean Soc Food Sci Nutr 27: 275-280
  18. Jung JY, Hur SS, Choi YH. 1999. Studies on the efficient extraction process of alginic acid in sea tangle. Food Engineering Progress 3: 90-97
  19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-269
  20. Kim WJ, Choi HS. 1994. Development of combined methods for effective extraction of sea mustard. Korean J Food Sci Technol 26: 44-50
  21. Lee GD, Kim JO, Joo GJ, Kwon JH. 2005. Optimum conditions for the extraction of effective substances from the stem of Opuntia fiscus-indica. Food Sci Biotechnol 14: 190-195
  22. Kim KH, Cheong JJ. 1984. Optimum conditions for extracting alginic acid from Undaria pinnatifida and amino acid composition of its extraction residue. Korean J Food Sci Technol 16: 336-340
  23. Jeong YJ. 2004. Optimization of extraction conditions on physicochemical properties of extracts from stem barks of Kalopanax pictus. Food Sci Biotechnol 13: 733-738
  24. Choi HS, Kim WJ. 1993. Effects of polysaccharide hydrolase on solids yields and viscosity of aqueous extracts of sea mustard. Korean J Food Sci Technol 25: 589-591
  25. Choi HS, Kim JG, Kim WJ. 1992. Effects of HCl, sugar, salt and sequestrants on some quality properties of aquaeous extracts of sea mustard. Korean J Food Sci Technol 24: 387-392

Cited by

  1. Gamma-irradiation Elimination of Brown Color from the Anti-inflammatory Agent Containing an Undaria pinnatifida Extract vol.42, pp.5, 2009,
  2. Quality and Antioxidant Characteristics of Granule Tea Prepared with Sea Tangle (Laminaria japonica) and Sea Mustard (Undaria pinnatifida) Powder as Affected by Extraction Method vol.19, pp.4, 2012,
  3. Effects of Dietary Fiber from Mozuku (Cladosiphon novae-caledoniae kylin) Residue on Antioxidant Activity and Anticancer in HT-29 Human Colon Cancer Cells according to Extraction Condition vol.25, pp.4, 2014,
  4. Quality characteristics of Hijikia fusiforme extracts with different extraction method vol.22, pp.1, 2015,